High-resolution hyperpolarized in vivo metabolic 13C spectroscopy at low magnetic field (48.7 mT) following murine tail-vein injection

Coffey, Aaron M.; Feldman, Matthew; Shchepin, Roman V.; Barskiy, Danila A.; Truong, Milton L.; Pham, Wellington; Chekmenev, Eduard Y.

doi:10.1016/j.jmr.2017.06.009

Cited by 27 publications

(37 citation statements)

References 79 publications

(73 reference statements)

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“…Clinically relevant tracers : The hitherto limited pool of agents amenable to p H 2 -HP was recently significantly extended by SABRE [ 42 ] and PHIP-SAH [ 24 ]. By now, molecules for receptor imaging [ 43 , 44 ], succinate [ 18 , 19 , 45 ], lactate [ 11 – 13 , 16 ], acetate [ 24 ] were polarized, and first metabolic studies were performed with PHIP-SAH-polarized pyruvate [ 11 , 24 , 46 ].…”

Section: Discussionmentioning

confidence: 99%

In vivo 13C-MRI using SAMBADENA

et al. 2018

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Magnetic Resonance Imaging (MRI) is a powerful imaging tool but suffers from a low sensitivity that severely limits its use for detecting metabolism in vivo. Hyperpolarization (HP) methods have demonstrated MRI signal enhancement by several orders of magnitude, enabling the detection of metabolism with a sensitivity that was hitherto inaccessible. While it holds great promise, HP is typically relatively slow (hours), expensive (million $, €) and requires a dedicated device (“polarizer”). Recently, we introduced a new method that creates HP tracers without an external polarizer but within the MR-system itself based on parahydrogen induced polarization (PHIP): Synthesis Amid the Magnet Bore Allows Dramatically Enhanced Nuclear Alignment (SAMBADENA). To date, this method is the simplest and least cost-intensive method for hyperpolarized 13C-MRI. HP of P13C > 20% was demonstrated for 5mM tracer solutions previously. Here, we present a setup and procedure that enabled the first in vivo application of SAMBADENA: Within seconds, a hyperpolarized angiography tracer was produced and injected into an adult mouse. Subsequently, fast 13C-MRI was acquired which exhibited the vena cava, aorta and femoral arteries of the rodent. This first SAMBADENA in vivo 13C-angiography demonstrates the potential of the method as a fast, simple, low-cost alternative to produce HP-tracers to unlock the vast but hidden powers of MRI.

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Section: Discussionmentioning

confidence: 99%

In vivo 13C-MRI using SAMBADENA

et al. 2018

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“…Unsaturated PHIP precursors were mixed with the Rh-based hydrogenation catalyst [54], and pH was then adjusted depending on the specific precursor under investigation ( e.g . by addition of HCl or phosphate buffer solutions [33, 67]). By holding valves v s1 , v s3 , v s5 closed, and actuating valves v s2 and v s6 (see Figure 1), the polarizer was flushed with N 2 (g) to clean and dry device fluid paths.…”

Section: Methodsmentioning

confidence: 99%

A pulse programmable parahydrogen polarizer using a tunable electromagnet and dual channel NMR spectrometer

Coffey

Shchepin

Feng

et al. 2017

Journal of Magnetic Resonance

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Applications of parahydrogen induced polarization (PHIP) often warrant conversion of the chemically-synthesized singlet-state spin order into net heteronuclear magnetization. In order to obtain optimal yields from the overall hyperpolarization process, catalytic hydrogenation must be tightly synchronized to subsequent radiofrequency (RF) transformations of spin order. Commercial NMR consoles are designed to synchronize applied waves on multiple channels and consequently are well-suited as controllers for these types of hyperpolarization experiments that require tight coordination of RF and non-RF events. Described here is a PHIP instrument interfaced to a portable NMR console operating with a static field electromagnet in the milliTesla regime. In addition to providing comprehensive control over chemistry and RF events, this setup condenses the PHIP protocol into a pulse-program that in turn can be readily shared in the manner of traditional pulse sequences. In this device, a TTL multiplexer was constructed to convert spectrometer TTL outputs into 24 VDC signals. These signals then activated solenoid valves to control chemical shuttling and reactivity in PHIP experiments. Consolidating these steps in a pulse-programming environment speeded calibration and improved quality assurance by enabling the B0/B1 fields to be tuned based on the direct acquisition of thermally polarized and hyperpolarized NMR signals. Performance was tested on the parahydrogen addition product of 2-hydroxyethyl propionate-1-13C-d3, where the 13C polarization was estimated to be P13C = 20 ± 2.5 % corresponding to 13C signal enhancement approximately 25 million-fold at 9.1 mT or approximately 77,000-fold 13C enhancement at 3 T with respect to thermally induced polarization at room temperature.

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“…The experiments were performed on both in vitro acetate phantoms and rat torso in vivo data. The linewidths obtained at this field were compared with those at 4.7T and found an improvement by a factor of 12 at the lower field …”

Section: Current State Of the Artmentioning

confidence: 99%

“…The linewidths obtained at this field were compared with those at 4.7T and found an improvement by a factor of 12 at the lower field. 64 Low Field (LF) MRI (0.2-0.5T) The increase in magnetic field strength compared with ULF and VLF MRI provides for higher SNR, and a compromise on the economics of purchase, siting, and service. Also, the SNR gain typically expedites the path to clinical evaluation of e70…”

Section: Ulf Mri (<10 Mt)mentioning

confidence: 99%

Accessible magnetic resonance imaging: A review

Geethanath

Vaughan

2019

Magnetic Resonance Imaging

137

139

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The role of MRI in diagnostics, prognostics, and discoveries in basic sciences has been well established. However, access to this life‐saving technology is largely restricted to countries in upper‐middle to high‐income groups. In this article, we collate recent global MR scanner density data and group them into six geographical regions based on the WHO classification. We then analyze these data with respect to demographic factors such as population size, life expectancy, the percentage of internet users, and World Bank income grouping. We map these demographic factors to five dimensions or characteristics of accessible MRI, adapting definitions from the healthcare literature. With this background, the study then reviews recent demonstrations of accessible MRI categorized based on main magnetic field strength. We describe demonstrated examples for each of these categories, ranging from ultralow‐field to ultrahigh‐field MRI. Lastly, we review MR methods and associated developments impacting accessible MRI such as increasing/augmenting MR awareness and local expertise, incorporating hardware‐cognizant methods, rapid quantitative imaging, and leveraging innovations from adjacent fields. Level of Evidence: 5 Technical Efficacy Stage: 6 J. Magn. Reson. Imaging 2019.

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High-resolution hyperpolarized in vivo metabolic 13C spectroscopy at low magnetic field (48.7 mT) following murine tail-vein injection

Cited by 27 publications

References 79 publications

In vivo 13C-MRI using SAMBADENA

In vivo 13C-MRI using SAMBADENA

A pulse programmable parahydrogen polarizer using a tunable electromagnet and dual channel NMR spectrometer

Accessible magnetic resonance imaging: A review

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