High resolution G-banding patterns of Syrian hamster chromosomes

Li, Shaoying; Pathak, Sen; Hsü, T. C.

doi:10.1159/000131775

Cited by 29 publications

(14 citation statements)

References 3 publications

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“…Chromosomes were analyzed by the quinacrine fluorescence staining method (8). Nomenclature of Syrian hamster chromosomes was according to Li et al (26). A total of 50 metaphases were examined for chromosome number, sex chromosomes, and marker chromosomes.…”

mentioning

confidence: 99%

Neoplastic transformation of normal and carcinogen-induced preneoplastic Syrian hamster embryo cells by the v-src oncogene.

Gilmer¹,

Annab²,

Oshimura³

et al. 1985

Mol. Cell. Biol.

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The ability of cloned Rous sarcoma virus (RSV) DNA encoding the v-src oncogene to neoplasticafly transform normal, diploid Syrian hamster embryo (SHE) cells was examined. Transfection of RSV DNA into early passage SHE cells resulted in a low but significant number of tumors when treated cells were injected into nude mice. Tumors formed with a low frequency (two tumors out of ten sites injected) and only after a long latency period (14 weeks). In contrast to the normal SHE cells, several different carcinogen-induced preneoplastic immortal SHE cell lines were highly susceptible to transformation by the v-src oncogene to the neoplastic phenotype. Tumors formed with high efficiency and a short latency period (<3 weeks). Further studies were performed to determine the basis for the inefficient transformation of the normal SHE cells. NeoR clones isolated after cotransfection of SHE cells with pSV2-neo and RSV DNAs were neither morphologically altered nor immortal and did not contain detectable levels of the v-src gene product. These results suggest that neoplastic transformation by v-src DNA in the normal cells is initially suppressed. However, cells from a v-src-induced tumor expressed v-src RNA, and antibody to v-src protein precipitated from the tumor cells a 60,000-molecular-weight protein which displayed protein kinase activity. Karyotypic analyses confirmed that the tumor was derived from Syrian hamster cells and suggested that it was clonal in nature. These results indicate that the v-src oncogene was primarily responsible for neoplastic transformation of SHE cells. In contrast to the results with the v-src oncogene, our previous studies showed that v-Ha-ras oncogene alone is unable to induce neoplastic transformation of SHE cells. Furthermore, the v-myc oncogene was able to complement v-Ha-ras to neoplastically transform SHE cells, while cotransfection with v-src plus v-myc did not increase the incidence of tumors.To understand the significance of oncogenes in neoplastic development, it is necessary to define the role of specific oncogenes in different steps of the multistep process of neoplastic progression. One experimental approach to this problem is the transfection of normal or preneoplastic cells with specific oncogenes or combinations of oncogenes to determine whether neoplastic transformation can be induced by this method. For example, Land et al. (23) To determine the universality of results obtained with a given system, it is important to compare the effects of transfection of oncogenes into different target cells. We have been studying the neoplastic progression of Syrian hamster embryo (SHE) cells in culture. This system has a number of advantages for studying the role of oncogenes in a multistep process of carcinogenesis. The normal cells have a stable karyotype and show a lower frequency of spontaneous transformation than do mouse, rat, or Chinese hamster fibroblasts (4). However, a variety of chemical carcinogens can induce morphologically transformed cells which can be isolated and cloned. The...

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mentioning

confidence: 99%

Neoplastic transformation of normal and carcinogen-induced preneoplastic Syrian hamster embryo cells by the v-src oncogene.

Gilmer¹,

Annab²,

Oshimura³

et al. 1985

Mol. Cell. Biol.

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“…G-banded chromosome patterns of the Syrian hamster (Mesocricetus auratus) have been described by several groups of investigators [Popescu and DiPaolo, 1972;Pavia et al, 1977;Pathak et al, 1981;Li et al, 1982]. Using confocal microscopy and a combination of DAPI-banding and FISH on the same metaphase spreads of the Syrian hamster, we mapped ITRs with strong FISH signals to the pericentromeric regions of chromosomes 2, 4, 14, 20 and X and specified the banding structure of these regions in comparison with that reported by Li and coworkers after G-banding [Li et al, 1982] ( figs.…”

Section: Resultsmentioning

confidence: 98%

“…The differences of relative intensity of the bands are shown by using 4 grey scale levels as accepted in modern cytogenetics [ISCN, 2009]. The nomenclature of individual bands corresponds to that of G-banded Syrian hamster chromosomes [Li et al, 1982]. The ideograms filled with dashed lines presented by these authors were modified in our work to halftone ideograms with 4 grey scale levels.…”

Section: Creating Ideograms Of Inversed Dapi-banded Chromosomesmentioning

confidence: 99%

High-Resolution Mapping of Interstitial Telomeric Repeats in Syrian Hamster Metaphase Chromosomes

Demin

Pleskach²,

Svetlova³

et al. 2010

Cytogenet Genome Res

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Karyotype analysis of the Syrian hamster (Mesocricetus auratus) was performed after DAPI-banding of metaphase chromosomes obtained from cultivated skin fibroblasts of a newborn animal. Fluorescence in situ hybridization with telomeric FITC-conjugated peptide nucleic acid probe was applied to map interstitial blocks of (TTAGGG)_n repeats. Strong fluorescence in situ hybridization signals corresponded to interstitial telomeric repeats in pericentromeric chromatin bands of chromosomes 2, 4, 14, 20, and X. High-resolution DAPI-banding allowed specifying the arrangement of bands in the pericentromeric regions of these chromosomes.

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“…They were stained with propidium iodide and observed using excitation at a wavelength of 450-490 nm (Nikon filter set B-2A) and near 365 nm (UV-2A). The ideograms of the Syrian and Chinese hamster chromosomes were derived from Li et al (1982) and Ray and Mohandas (1976), respectively.…”

Section: Physical Mapping Of the Ku70 And Ku80 Genes By Direct R-bandmentioning

confidence: 99%

Expression and chromosome location of hamster <i>Ku70</i> and <i>Ku80</i>

Koike¹,

Kuroiwa

Koike³

et al. 2001

Cytogenet Genome Res

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Ku proteins play an important role in DNA double-strand break (DSB) repair, chromosome maintenance, and growth regulation. To understand the fundamental characteristics of Ku proteins, we examined the electrophoretic mobility and expression of hamster Ku70 and Ku80 and determined the chromosome locations of their genes. The electrophoretic mobility of hamster Ku proteins are different from that of human Ku proteins. No significant changes in the quantity of Ku proteins were observed in CHO-K1 cells treated with 10 Gy of ionizing radiation, suggesting that both proteins are expressed constitutively in amounts adequate to repair DNA DSBs. The chromosome locations of the Ku genes were determined by direct R-banding fluorescence in situ hybridization. The Ku70 gene was localized to Syrian hamster chromosome 4qa4.1→ qa4.2 and Chinese hamster chromosome 2p3.1, and the Ku80 gene was localized to Syrian hamster chromosome 4qb5→ qb6.1 and Chinese hamster chromosome 2p3.5→p3.6. These results provide clues to the biological functions of Ku, as well as useful information for constructing comparative chromosome maps between hamsters and other mammalian species, including human, mouse, and rat.

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High resolution G-banding patterns of Syrian hamster chromosomes

Cited by 29 publications

References 3 publications

Neoplastic transformation of normal and carcinogen-induced preneoplastic Syrian hamster embryo cells by the v-src oncogene.

Neoplastic transformation of normal and carcinogen-induced preneoplastic Syrian hamster embryo cells by the v-src oncogene.

High-Resolution Mapping of Interstitial Telomeric Repeats in Syrian Hamster Metaphase Chromosomes

Expression and chromosome location of hamster <i>Ku70</i> and <i>Ku80</i>

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