High-resolution fluorescence-guided transcranial ultrasound mapping in the live mouse brain

Estrada, Héctor; Robin, Justine; Özbek, Ali; Chen, Zhenyue; Marowsky, Anne; Zhou, Quanyu; Beck, Daniel; Roy, Beau le; Arand, Michael; Shoham, Shy; Razansky, Daniel

doi:10.1126/sciadv.abi5464

Cited by 18 publications

(26 citation statements)

References 50 publications

(76 reference statements)

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“…Technical advancements, such as the development of less invasive approaches, are particularly important for implementing such investigations. For example, extracranial delivery of optogenetic (Chen et al, 2021) and ultrasonic stimulations (Estrada et al, 2021) as well as wireless recording capabilities (Shin et al, 2022) will make observation and manipulation of cell type specific populations during freely moving task engagement less cumbersome.…”

Section: Discussionmentioning

confidence: 99%

Parvalbumin-Positive Interneurons Regulate Cortical Sensory Plasticity in Adulthood and Development Through Shared Mechanisms

Rupert

Shea

2022

Front. Neural Circuits

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Parvalbumin-positive neurons are the largest class of GABAergic, inhibitory neurons in the central nervous system. In the cortex, these fast-spiking cells provide feedforward and feedback synaptic inhibition onto a diverse set of cell types, including pyramidal cells, other inhibitory interneurons, and themselves. Cortical inhibitory networks broadly, and cortical parvalbumin-expressing interneurons (cPVins) specifically, are crucial for regulating sensory plasticity during both development and adulthood. Here we review the functional properties of cPVins that enable plasticity in the cortex of adult mammals and the influence of cPVins on sensory activity at four spatiotemporal scales. First, cPVins regulate developmental critical periods and adult plasticity through molecular and structural interactions with the extracellular matrix. Second, they activate in precise sequence following feedforward excitation to enforce strict temporal limits in response to the presentation of sensory stimuli. Third, they implement gain control to normalize sensory inputs and compress the dynamic range of output. Fourth, they synchronize broad network activity patterns in response to behavioral events and state changes. Much of the evidence for the contribution of cPVins to plasticity comes from classic models that rely on sensory deprivation methods to probe experience-dependent changes in the brain. We support investigating naturally occurring, adaptive cortical plasticity to study cPVin circuits in an ethologically relevant framework, and discuss recent insights from our work on maternal experience-induced auditory cortical plasticity.

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Section: Discussionmentioning

confidence: 99%

Parvalbumin-Positive Interneurons Regulate Cortical Sensory Plasticity in Adulthood and Development Through Shared Mechanisms

Rupert

Shea

2022

Front. Neural Circuits

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“…28 The simulations were calibrated using hydrophone measurements performed under full water immersion in a water tank. 23,25 Simulations of the spatio-temporal heat deposition dynamics induced by FUS are modeled using the bioheat model 29 as implemented by Soneson. 30 The input to the model corresponds to the spatial US field distribution using the same parameters used in the experiments with the thermal constants adapted from the literature.…”

Section: Simulationsmentioning

confidence: 99%

“…22 Recently, we integrated high-resolution FUS delivery and simultaneous widefield fluorescence imaging to achieve and characterize highly precise FUS targeting in a living mouse brain. 23 However, our initial observations were dominated by the thermal dynamics of fluoro-thermal tags (FTT) or propagating spreading depolarizations, and no localized neural activity has been isolated. Here, we further advance the ability of cortex-wide fluorescence imaging to observe responses to precisely steered localized FUS stimuli by characterizing the activation dynamics and developing a method to separate thermal fluorescence quenching and actual neural responses.…”

Section: Introductionmentioning

confidence: 99%

Model-based correction of rapid thermal confounds in fluorescence neuroimaging of targeted perturbation

Davoudi,

Estrada,

Özbek

et al. 2024

Neurophoton.

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An array of techniques for targeted neuromodulation is emerging, with high potential in brain research and therapy. Calcium imaging or other forms of functional fluorescence imaging are central solutions for monitoring cortical neural responses to targeted neuromodulation, but often are confounded by thermal effects that are inter-mixed with neural responses.Aim: Here, we develop and demonstrate a method for effectively suppressing fluorescent thermal transients from calcium responses.Approach: We use high precision phased-array 3 MHz focused ultrasound delivery integrated with fiberscope-based widefield fluorescence to monitor cortex-wide calcium changes. Our approach for detecting the neural activation first takes advantage of the high inter-hemispheric correlation of resting state Ca 2þ dynamics and then removes the ultrasound-induced thermal effect by subtracting its simulated spatio-temporal signature from the processed profile. Results:The focused 350 μm-sized ultrasound stimulus triggered rapid localized activation events dominated by transient thermal responses produced by ultrasound. By employing bioheat equation to model the ultrasound heat deposition, we can recover putative neural responses to ultrasound. Conclusions:The developed method for canceling transient thermal fluorescence quenching could also find applications with optical stimulation techniques to monitor thermal effects and disentangle them from neural responses. This approach may help deepen our understanding of the mechanisms and macroscopic effects of ultrasound neuromodulation, further paving the way for tailoring the stimulation regimes toward specific applications.

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“…Despite its important advantages, OAT often provides insufficient soft-tissue contrast, complicating anatomical interpretation of the data. Due to the inherent hybrid optical and ultrasound nature of OAT, multi-modality efforts have thus far been focused on hybridization with ultrasound 24 – 26 , fluorescence imaging 18 , 27 – 29 , or optical coherence tomography (OCT) 30 – 32 . Registration of OAT images with stand-alone computerized tomography (CT), single-photon emission computerized tomography (SPECT), and MRI has also been reported 4 , 33 , 34 .…”

Section: Introductionmentioning

confidence: 99%

Hybrid magnetic resonance and optoacoustic tomography (MROT) for preclinical neuroimaging

et al. 2022

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Multi-modal imaging is essential for advancing our understanding of brain function and unraveling pathophysiological processes underlying neurological and psychiatric disorders. Magnetic resonance (MR) and optoacoustic (OA) imaging have been shown to provide highly complementary contrasts and capabilities for preclinical neuroimaging. True integration between these modalities can thus offer unprecedented capabilities for studying the rodent brain in action. We report on a hybrid magnetic resonance and optoacoustic tomography (MROT) system for concurrent noninvasive structural and functional imaging of the mouse brain. Volumetric OA tomography was designed as an insert into a high-field MR scanner by integrating a customized MR-compatible spherical transducer array, an illumination module, and a dedicated radiofrequency coil. A tailored data processing pipeline has been developed to mitigate signal crosstalk and accurately register image volumes acquired with T1-weighted, angiography, and blood oxygenation level-dependent (BOLD) sequences onto the corresponding vascular and oxygenation data recorded with the OA modality. We demonstrate the concurrent acquisition of dual-mode anatomical and angiographic brain images with the scanner, as well as real-time functional readings of multiple hemodynamic parameters from animals subjected to oxygenation stress. Our approach combines the functional and molecular imaging advantages of OA with the superb soft-tissue contrast of MR, further providing an excellent platform for cross-validation of functional readings by the two modalities.

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High-resolution fluorescence-guided transcranial ultrasound mapping in the live mouse brain

Abstract: Precision targeting and monitoring of preclinical focused ultrasound enabled by fluorescence-guided transcranial ultrasound.

Cited by 18 publications

References 50 publications

Parvalbumin-Positive Interneurons Regulate Cortical Sensory Plasticity in Adulthood and Development Through Shared Mechanisms

Parvalbumin-Positive Interneurons Regulate Cortical Sensory Plasticity in Adulthood and Development Through Shared Mechanisms

Model-based correction of rapid thermal confounds in fluorescence neuroimaging of targeted perturbation

Hybrid magnetic resonance and optoacoustic tomography (MROT) for preclinical neuroimaging

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