High Resolution Fiber-Fluorescence In Situ Hybridization

Ye, Christine J.; Heng, Henry H.Q.

doi:10.1007/978-1-4939-6703-2_14

Cited by 8 publications

(1 citation statement)

References 41 publications

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“…The Fiber-FISH is often used for determining size-gapping problems, locating delections, resolving chromatin breakpoints linked to diseases, estimating gene copy number variations, orientations, genes length, etc. [ 86 , 87 ].…”

Section: Discussionmentioning

confidence: 99%

State of the art on the physical mapping of the Y-chromosome in the Bovidae and comparison with other species — A review

et al. 2022

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The next generation sequencing (NGS) has significantly contributed to clarify the genome structure of many species of zootechnical interest. However, to date, some portions of the genome, especially those linked to a heterogametic nature such as the Y chromosome, are difficult to assemble and many gaps are still present. It is well known that the fluorescence in situ hybridization (FISH) is an excellent tool for identifying genes unequivocably mapped on chromosomes. Therefore, FISH can contribute to the localization of unplaced genome sequences, as well as to correct assembly errors generated by comparative bioinformatics. To this end, it is necessary to have starting points; therefore, in this study, we reviewed the physically mapped genes on the Y chromosome of cattle, buffalo, sheep, goats, pigs, horses and alpacas. A total of 208 loci were currently mapped by FISH. 89 were located in the malespecific region of the Y chromosome (MSY) and 119 were identified in the pseudoautosomal region (PAR). The loci reported in MSY and PAR were respectively: 18 and 25 in BTA, 5 and 7 in BBU, 5 and 24 in OAR, 5 and 19 in CHI, 10 and 16 in SSC, 46 and 18 in ECA. While in VPA only 10 loci are reported in the PAR region.The correct knowledge and assembly of all genome sequences, including those of genes mapped on the Y chromosome, will help to elucidate their biological processes, as well as to discover and exploit potentially epistasis effects useful for selection breeding programs.

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Section: Discussionmentioning

confidence: 99%

State of the art on the physical mapping of the Y-chromosome in the Bovidae and comparison with other species — A review

et al. 2022

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The Importance of Monitoring Non-clonal Chromosome Aberrations (NCCAs) in Cancer Research

Heng,

Thanedar,

Heng

2024

Methods in Molecular Biology

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Experimental Induction of Genome Chaos

Heng

2018

Methods in Molecular Biology

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Genome chaos, or karyotype chaos, represents a powerful survival strategy for somatic cells under high levels of stress/selection. Since the genome context, not the gene content, encodes the genomic blueprint of the cell, stress-induced rapid and massive reorganization of genome topology functions as a very important mechanism for genome (karyotype) evolution. In recent years, the phenomenon of genome chaos has been confirmed by various sequencing efforts, and many different terms have been coined to describe different subtypes of the chaotic genome including "chromothripsis," "chromoplexy," and "structural mutations." To advance this exciting field, we need an effective experimental system to induce and characterize the karyotype reorganization process. In this chapter, an experimental protocol to induce chaotic genomes is described, following a brief discussion of the mechanism and implication of genome chaos in cancer evolution.

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High Resolution Fiber-Fluorescence In Situ Hybridization

Cited by 8 publications

References 41 publications

State of the art on the physical mapping of the Y-chromosome in the Bovidae and comparison with other species — A review

State of the art on the physical mapping of the Y-chromosome in the Bovidae and comparison with other species — A review

The Importance of Monitoring Non-clonal Chromosome Aberrations (NCCAs) in Cancer Research

Experimental Induction of Genome Chaos

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