High resolution DNA melting markers for identification of H1-linked resistance to potato cyst nematode

Meiyalaghan, Sathiyamoorthy; Paget, M. F.; Thompson, Shirley; Thomson, Susan; Baldwin, Samantha; Anderson, John A.; Genet, R. A.; Lewthwaite, Stephen

doi:10.1007/s11032-018-0832-z

Cited by 7 publications

(3 citation statements)

References 34 publications

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“…Genetic analysis has been reported to be an efficient tool for screening for PCN resistance in a number of studies (Asano et al 2021, Biryukova et al 2008, Milczarek et al 2011, 2014, Schultz et al 2012, Zoteyeva et al 2020. PCR-based methods are less time-consuming than phenotypic tests, require less plant material, are easily repeatable and also more informative with allelic dosage (Meiyalaghan et al 2018). We chose to analyse the markers for gene H1, which provides near-absolute resistance against G. rostochiensis and is widely implemented in commercial potato cultivars (Finkers-Tomczak et al 2011, Toxopeus andHuijsman 1953).…”

Section: Suitability Of Genetic Markers For G Rostochiensis Resistanc...mentioning

confidence: 99%

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Studies of potato resistance to <i>Globodera rostochiensis</i> revealed novel alleles for 57R marker

Ivanova-Pozdejeva,

Jakobson,

Ilves

et al. 2023

Breed. Sci.

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Globodera rostochiensis resistance has been an important trait in potato (Solanum tuberosum) breeding for decades. Our aim was to complement phenotypic testing with genetic marker analysis. We analysed the results of G. rostochiensis resistance greenhouse testing in 4601 tubers of 2918 breeding clones from 11 years. Applicability of H1 gene markers TG689 and 57R was compared. We implemented the latter with the positive predictive value of 99.1% and negative predictive value of 60.0% into the breeding scheme. The 57R marker alleles of 22 Estonian cultivars and 470 breeding clones were determined. Two unique 57R alleles, 57R-887 and 57R-1155, were found in Estonian cultivar 'Anti'. The 887 bp allele has two deletions (14 bp and 490 bp) accompanied by several other indels and SNPs within the 57R marker region. The 1155 bp allele has three deletions (7 bp, 20 bp and 210 bp) accompanied by several other indels and SNPs within the same region. Partial resistance to G. rostochiensis in 'Anti' suggests that the newly described alleles could affect the H1-mediated resistance directly or indirectly.

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Section: Suitability Of Genetic Markers For G Rostochiensis Resistanc...mentioning

confidence: 99%

“…This gene provides near-absolute resistance to pathotypes Ro1 and Ro4 (Bakker et al 2004). PCR-based markers that have been developed for H1 detection include TG689, TG689_1P, 57R, N146, N195 and CP113 (Meiyalaghan et al 2018, Ramakrishnan et al 2015, Schultz et al 2012.…”

Section: Introductionmentioning

confidence: 99%

Studies of potato resistance to <i>Globodera rostochiensis</i> revealed novel alleles for 57R marker

Ivanova-Pozdejeva,

Jakobson,

Ilves

et al. 2023

Breed. Sci.

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“…For example, at a field-based germplasm collection in New Zealand genomic DNA was extracted and screened for the presence of TG689 and 57R haplotypes linked to the H1 gene. These haplotypes act as potential predictors for the resistance against a pathotype of potato cyst nematode (Globodera rostochiensis), an economically important pest [31]. Similarly, banana germplasm was screened for the resistance against the Fusarium oxysporum f. sp.…”

Section: Advantage Of Field Collections: Characterization and Evaluationmentioning

confidence: 99%

Challenges and Prospects for the Conservation of Crop Genetic Resources in Field Genebanks, in In Vitro Collections and/or in Liquid Nitrogen

Panis

Nagel

houwe

2020

Plants

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The conservation of crop genetic resources, including their wild relatives, is of utmost importance for the future of mankind. Most crops produce orthodox seeds and can, therefore, be stored in seed genebanks. However, this is not an option for crops and species that produce recalcitrant (non-storable) seeds such as cacao, coffee and avocado, for crops that do not produce seeds at all; therefore, they are inevitably vegetatively propagated such as bananas, or crops that are predominantly clonally propagated as their seeds are not true to type, such as potato, cassava and many fruit trees. Field, in vitro and cryopreserved collections provide an alternative in such cases. In this paper, an overview is given on how to manage and setup a field, in vitro and cryopreserved collections, as well as advantages and associated problems taking into account the practical, financial and safety issues in the long-term. In addition, the need for identification of unique accessions and elimination of duplicates is discussed. The different conservation methods are illustrated with practical examples and experiences from national and international genebanks. Finally, the importance of establishing safe and long-term conservation methods and associated backup possibilities is highlighted in the frame of the global COVID-19 pandemic.

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On the Value of Wild Solanum Species for Improved Crop Disease Resistance: Resistances to Nematodes and Viruses

Bradeen

2021

Compendium of Plant Genomes

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High resolution DNA melting markers for identification of H1-linked resistance to potato cyst nematode

Cited by 7 publications

References 34 publications

Studies of potato resistance to <i>Globodera rostochiensis</i> revealed novel alleles for 57R marker

Studies of potato resistance to <i>Globodera rostochiensis</i> revealed novel alleles for 57R marker

Challenges and Prospects for the Conservation of Crop Genetic Resources in Field Genebanks, in In Vitro Collections and/or in Liquid Nitrogen

On the Value of Wild Solanum Species for Improved Crop Disease Resistance: Resistances to Nematodes and Viruses

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