High-resolution beta imaging

Barthe, Nicole; Maitrejean, S.; Carvou, Nicolas; Cardona, Ana

doi:10.1016/b978-0-12-814395-7.00009-x

Cited by 7 publications

(8 citation statements)

References 111 publications

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“…As a digital autoradiography device, we used the Micro Imager (Biospace Lab S.A., Paris, France). Exposures were 20 min each and the counts were accumulated in list mode format (which records the time for each particle interaction in addition to its coordinates) allowing to test the quality of images with arbitrarily shorter exposure times.…”

Section: Methodsmentioning

confidence: 99%

Technical Note: Scintillation well counters and particle counting digital autoradiography devices can be used to detect activities associated with genomic profiling adequacy of biopsy specimens obtained after a low activity ¹⁸F‐FDG injection

et al. 2018

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Section: Methodsmentioning

confidence: 99%

Technical Note: Scintillation well counters and particle counting digital autoradiography devices can be used to detect activities associated with genomic profiling adequacy of biopsy specimens obtained after a low activity ¹⁸F‐FDG injection

et al. 2018

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“…Autoradiography is another technique that has been used to observe changes in brain cell receptor expression or architecture [146][147][148]. Here, a radioactive tracer is injected into the sample prior to brain isolation and exposure of a radiation sensitive imager plate [149]. This technique provides good data on the regional localization of the target of a given radioactive tracer.…”

Section: Metabolic Imaging Of the Brainmentioning

confidence: 99%

“…Depending on the tracer, inferences can then be made on changes in metabolism. This technique has been applied to analyze changes in PD, AD, and TBI [146][147][148][149]. This technique is particularly useful to determine drug or ligand occupancy of specific-receptors and is therefore a gold-standard technique in pharmacological studies evaluating novel drugs, and their targets [150,151].…”

Section: Metabolic Imaging Of the Brainmentioning

confidence: 99%

Altered substrate metabolism in neurodegenerative disease: new insights from metabolic imaging

Cleland

Al-Juboori

Dobrinskikh

et al. 2021

J Neuroinflammation

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Neurodegenerative diseases (NDs), such as Alzheimer’s disease (AD), Parkinson’s disease (PD) and multiple sclerosis (MS), are relatively common and devastating neurological disorders. For example, there are 6 million individuals living with AD in the United States, a number that is projected to grow to 14 million by the year 2030. Importantly, AD, PD and MS are all characterized by the lack of a true disease-modifying therapy that is able to reverse or halt disease progression. In addition, the existing standard of care for most NDs only addresses the symptoms of the disease. Therefore, alternative strategies that target mechanisms underlying the neuropathogenesis of disease are much needed. Recent studies have indicated that metabolic alterations in neurons and glia are commonly observed in AD, PD and MS and lead to changes in cell function that can either precede or protect against disease onset and progression. Specifically, single-cell RNAseq studies have shown that AD progression is tightly linked to the metabolic phenotype of microglia, the key immune effector cells of the brain. However, these analyses involve removing cells from their native environment and performing measurements in vitro, influencing metabolic status. Therefore, technical approaches that can accurately assess cell-specific metabolism in situ have the potential to be transformative to our understanding of the mechanisms driving AD. Here, we review our current understanding of metabolism in both neurons and glia during homeostasis and disease. We also evaluate recent advances in metabolic imaging, and discuss how emerging modalities, such as fluorescence lifetime imaging microscopy (FLIM) have the potential to determine how metabolic perturbations may drive the progression of NDs. Finally, we propose that the temporal, regional, and cell-specific characterization of brain metabolism afforded by FLIM will be a critical first step in the rational design of metabolism-focused interventions that delay or even prevent NDs.

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“…The radiation from radioisotopes interacts with the photographic emulsion in contact with the sample, thus providing an image of the distribution of the radioactivity in the sample. The word “autoradiography” comes from the fact that the sample is not placed between the source of radiation and the detector, as it is in radiography, but itself contains the source of radiation [ 14 ].…”

Section: Labeling Of Replicated Dna By Isotopically Labeled Nucleomentioning

confidence: 99%

DNA Replication: From Radioisotopes to Click Chemistry

Ligasová

Koberna

2018

Molecules

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The replication of nuclear and mitochondrial DNA are basic processes assuring the doubling of the genetic information of eukaryotic cells. In research of the basic principles of DNA replication, and also in the studies focused on the cell cycle, an important role is played by artificially-prepared nucleoside and nucleotide analogues that serve as markers of newly synthesized DNA. These analogues are incorporated into the DNA during DNA replication, and are subsequently visualized. Several methods are used for their detection, including the highly popular click chemistry. This review aims to provide the readers with basic information about the various possibilities of the detection of replication activity using nucleoside and nucleotide analogues, and to show the strengths and weaknesses of those different detection systems, including click chemistry for microscopic studies.

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High-resolution beta imaging

Cited by 7 publications

References 111 publications

Technical Note: Scintillation well counters and particle counting digital autoradiography devices can be used to detect activities associated with genomic profiling adequacy of biopsy specimens obtained after a low activity ¹⁸F‐FDG injection

Technical Note: Scintillation well counters and particle counting digital autoradiography devices can be used to detect activities associated with genomic profiling adequacy of biopsy specimens obtained after a low activity ¹⁸F‐FDG injection

Altered substrate metabolism in neurodegenerative disease: new insights from metabolic imaging

DNA Replication: From Radioisotopes to Click Chemistry

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High-resolution beta imaging

Cited by 7 publications

References 111 publications

Technical Note: Scintillation well counters and particle counting digital autoradiography devices can be used to detect activities associated with genomic profiling adequacy of biopsy specimens obtained after a low activity 18F‐FDG injection

Technical Note: Scintillation well counters and particle counting digital autoradiography devices can be used to detect activities associated with genomic profiling adequacy of biopsy specimens obtained after a low activity 18F‐FDG injection

Altered substrate metabolism in neurodegenerative disease: new insights from metabolic imaging

DNA Replication: From Radioisotopes to Click Chemistry

Contact Info

Product

Resources

About

Technical Note: Scintillation well counters and particle counting digital autoradiography devices can be used to detect activities associated with genomic profiling adequacy of biopsy specimens obtained after a low activity ¹⁸F‐FDG injection

Technical Note: Scintillation well counters and particle counting digital autoradiography devices can be used to detect activities associated with genomic profiling adequacy of biopsy specimens obtained after a low activity ¹⁸F‐FDG injection