High resolution and fast chemical imaging using widefield optical photothermal infrared microscope
Subham Adak,
Anooj Thayyil Raveendran,
Jürgen Popp
et al.
Abstract:Label-free infrared (IR) imaging reveals chemical bonds in specimens, but its limitations include a spatial resolution of approximately 5 µm. The advent of optical photothermal infrared (OPTIR) microscopy extends molecular fingerprint imaging by one order of magnitude near 500 nm. However, traditional scanning OPTIR faces a challenge of imaging speed. To improve speed and throughput, we employ pulsed visible probe light for widefield detection of transient photothermal responses induced by mid-infrared pulses.… Show more
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