High‐resolution analyses of cell fusion dynamics in a biochip

Mottet, Guillaume; Pioufle, Bruno Le; Mir, Lluis M.

doi:10.1002/elps.201200112

Cited by 3 publications

(2 citation statements)

References 48 publications

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“…Electric field is often used as a mean to handle, treat, or monitor living cells . AC electrokinetic phenomena such as dielectrophoresis, traveling wave dielectrophoresis and electrorotation became largely investigated in lab‐on‐chip and microfluidic devices .…”

Section: Introductionmentioning

confidence: 99%

Monitoring the permeabilization of a single cell in a microfluidic device, through the estimation of its dielectric properties based on combined dielectrophoresis and electrorotation in situ experiments

2015

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The electric field is commonly used in microdevices to handle, treat, or monitor living cells for various biological or biomedical applications (cells electrofusion, gene electrotransfer, drugs injection, cell sorting, …). Dielectrophoresis (DEP) forces, using stationary waves (conventional DEP) or traveling waves, are widely used for the cell handling or sorting. Electrorotation, which is induced by a rotating electrical field, is used for the determination of cell dielectric parameters. The application of pulsed electric field (PEF) results in the cell membrane permeabilization that might allow the transfer of various molecules in the cytoplasm. In this paper, we propose a method to monitor in situ the level of electropermeabilization induced by PEF application on a single cell, by combining the dielectrophoresis force and the electrorotation torque within a microfluidic device. The method was experimented on two different cell lines (human leukemic T-cell lymphoblast and murine melanoma cell): a single cell is captured by dielectrophoresis while its dielectric properties (both permittivity and conductivity of cytoplasm and membrane) are estimated thanks to a rotating electric field, which is applied simultaneously. The permeabilization effect of PEF, applied to the single cell trapped in such conditions in the biodevice, could be monitored by the estimation of its dielectric properties before and after pulse application.

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Section: Introductionmentioning

confidence: 99%

Monitoring the permeabilization of a single cell in a microfluidic device, through the estimation of its dielectric properties based on combined dielectrophoresis and electrorotation in situ experiments

2015

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“…16,[20][21][22][23][24] The last two methods are very efficient for cell pairing and electrofusion but are both coupled with mechanical handling (fluidic and mechanic in the case of Ref. 9, electric and mechanic in the case of Refs.…”

Section: Introductionmentioning

confidence: 99%

Microarray of non-connected gold pads used as high density electric traps for parallelized pairing and fusion of cells

et al. 2013

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Cell fusion consists of inducing the formation of a hybridoma cell containing the genetic properties of the progenitor cells. Such an operation is usually performed chemically or electrically. The latter method, named electrofusion, is considered as having a strong potential, due to its efficiency and non-toxicity, but deserves further investigations prior to being applicable for key applications like antibody production and cancer immunotherapy. Indeed, to envision such applications, a high amount of hybrid cells is needed. In this context, we present in this paper a device for massive cell pairing and electrofusion, using a microarray of nonconnected conductive pads. The electrofusion chamber--or channel--exposes cells to an inhomogeneous electric field, caused by the pads array, enabling the trapping and pairing of cells with dielectrophoresis (DEP) forces prior to electrofusion. Compared to a mechanical trapping, such electric trapping is fully reversible (on/ off handling). The DEP force is contactless and thus eases the release of the produced hybridoma. Moreover, the absence of wire connections on the pads permits the high density trapping and electrofusion of cells. In this paper, the electric field mapping, the effect of metallic pads thickness, and the transmembrane potential of cells are studied based on a numerical model to optimize the device. Electric calculations and experiments were conducted to evaluate the trapping force. The structure was finally validated for cell pairing and electrofusion of arrays of cells. We believe that our approach of fully electric trapping with a simple structure is a promising method for massive production of electrofused hybridoma. V C 2013 AIP Publishing LLC. [http://dx

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Analysis of pulsed electric field effects on cellular tissue with Cole–Cole model: Monitoring permeabilization under inhomogeneous electrical field with bioimpedance parameter variations

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Pioufle

2015

Innovative Food Science & Emerging Technologies

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High‐resolution analyses of cell fusion dynamics in a biochip

Cited by 3 publications

References 48 publications

Monitoring the permeabilization of a single cell in a microfluidic device, through the estimation of its dielectric properties based on combined dielectrophoresis and electrorotation in situ experiments

Monitoring the permeabilization of a single cell in a microfluidic device, through the estimation of its dielectric properties based on combined dielectrophoresis and electrorotation in situ experiments

Microarray of non-connected gold pads used as high density electric traps for parallelized pairing and fusion of cells

Analysis of pulsed electric field effects on cellular tissue with Cole–Cole model: Monitoring permeabilization under inhomogeneous electrical field with bioimpedance parameter variations

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