Recent studies have demonstrated that positron emission tomography (PET) is a valuable tool for in-situ characterization of fluid transport in porous and fractured geologic media at the laboratory scale. While PET imaging is routinely used for clinical cancer diagnosis and preclinical medical researchand therefore imaging facilities are available at most research instituteswidespread adoption for applications in water resources and subsurface energy resources engineering have been limited by real and perceived challenges of working with this technique. In this study we discuss and address these challenges, and provide detailed analysis highlighting how positron emission tomography can complement and improve laboratory characterization of different subsurface fluid transport problems. The physics of PET are reviewed to provide a fundamental understanding of the sources of noise, resolution limits, and safety considerations. We then layout the methodology required to perform laboratory experiments imaged with PET, including a new protocol for radioactivity dosing optimization for imaging in geologic materials. Signal-to-noise and sensitivity analysis comparisons between PET and clinical X-ray computed tomography are performed to highlight how PET data can complement more traditional characterization methods, particularly for solute transport problems. Finally, prior work is critically reviewed and discussed to provide a better understanding of the strengths and weakness of PET and how to best utilize PET-derived data for future studies. in many geologic materials can limit the application of MRI imaging in ge-35 ologic porous and fractured media (Reeves and Chudek, 2001; Werth et al., 36 2010; Nestle et al., 2003). Emission tomography includes two main imaging 37 techniques, positron emission tomography (PET) and single-photon emis-38 sion computed tomography (SPECT), that rely on photon emission for in 39 situ imaging. Positron emission tomography is the most common these emis-40 sion tomography methods and will be the focus of this study. PET relies on 41 the emission and detection of photons from positron-emitting radiotracers 42 using cylindrical arrays of scintillation crystals. Tomographic reconstruction 43 methods are used to obtain three-dimensional images of radiotracer distri-44 bution in the porous material as a function of time. Similarly to X-ray CT 45 and MRI, PET has been developed for medical purposes, but is increasingly 46 being used for applications in engineering and Earth science (Ferno et al., 47 2015b; Pini et al., 2016; Brattekas and Seright, 2017; Zahasky et al., 2018; 48 Kulenkamp↵ et al., 2018). 49 The distinct physics that underlie these imaging techniques determine 50 their strengths and weaknesses for various applications in water resources 51 and subsurface energy resources engineering. While X-ray computed tomog-52 raphy has become ubiquitous in geoscience experimental applications (e.g. 53 porosity distribution measurements shown in Figure 1), PET imaging has 54 had limited utilizat...