High resistance of fluoroquinolone and macrolide reported in avian pathogenic Escherichia coli isolates from the humid subtropical regions of Pakistan

Jalil, Amna; Masood, Saima; Ain, Quratul; Andleeb, Saadia; Dudley, Edward G.; Adnan, Fazal

doi:10.1016/j.jgar.2023.01.009

Cited by 2 publications

(1 citation statement)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the effectiveness and necessity of this approach remain subjects of debate within the medical community (8). discussion by specifically investigating the impact of prophylactic antibiotics on the reduction of hospital stay in ischemic stroke patients (10,11).…”

Section: Introductionmentioning

confidence: 99%

Impact of Prophylactic Antibiotics in Ischemic Stroke Patients on Reduction of Hospital Stay.

Mehroon Razzaq,

Khan,

Hussain

et al. 2023

JHRR

View full text Add to dashboard Cite

Background: Ischemic stroke is a leading cause of morbidity and mortality, with post-stroke infections significantly impacting patient outcomes. The use of prophylactic antibiotics to prevent these infections and reduce hospital stay durations has been a subject of debate in the medical community. Objective: This study aims to evaluate the impact of prophylactic antibiotic administration on the length of hospital stays in ischemic stroke patients. Methods: Conducted at the Combined Military Hospital, Peshawar, this randomized control trial involved 115 patients, divided into two groups. Group 1 (55 patients) received prophylactic ceftriaxone, while Group 2 (60 patients) did not receive antibiotics. Inclusion criteria were a Glasgow Coma Scale (GCS) score 13 and above, with no pre-existing conditions necessitating antibiotics or complications from the stroke. The primary outcome was the duration of hospital stay. Results: Group 1 had an average hospital stay of 6.65 days (SD: 2.06 days), while Group 2 had an average of 6.81 days (SD: 1.98 days). The P value of 0.677 indicated no statistically significant difference in hospital stay durations between the groups. Both groups had similar age and GCS score distributions. Conclusion: The administration of prophylactic antibiotics to ischemic stroke patients did not significantly reduce hospital stay durations. Given the risks associated with antibiotic resistance and side effects, a patient-specific approach to prophylactic antibiotic use is recommended. Future research should focus on identifying specific patient subgroups that may benefit from prophylactic antibiotics and optimal timing for their administration.

show abstract

Section: Introductionmentioning

confidence: 99%

Impact of Prophylactic Antibiotics in Ischemic Stroke Patients on Reduction of Hospital Stay.

Mehroon Razzaq,

Khan,

Hussain

et al. 2023

JHRR

View full text Add to dashboard Cite

show abstract

Potential of ZnO nanoparticles for multi-drug resistant Escherichia coli having CRISPR-Cas from poultry market in Lahore

Shabbir,

Shamim,

Tahir

et al. 2024

BMC Microbiol

View full text Add to dashboard Cite

Background and objectives Apart from known factors such as irrational use of antibiotics and horizontal gene transfer, it is now reported that clustered regularly interspaced short palindromic repeats (CRISPR) are also associated with increased antimicrobial resistance. Hence, it is critical to explore alternatives to antibiotics to control economic losses. Therefore, the present study aimed to determine not only the association of CRISPR-Cas system with antibiotic resistance but also the potential of Zinc Oxide nanoparticles (ZnO-NPs) for avian pathogenic Escherichia coli (APEC) isolated from poultry market Lahore. Materials and methods Samples ( n = 100) were collected from live bird markets of Lahore, and isolates were confirmed as Escherichia coli ( E. coli ) using the Remel One fast kit, and APEC was identified using PCR. The antibiotic resistance pattern in APEC was determined using the minimum inhibitory concentration (MIC), followed by genotypic confirmation of antibiotic-resistant genes using the PCR. The CRISPR-Cas system was also identified in multidrug-resistant (MDR) isolates, and its association with antibiotics was determined using qRT-PCR. The potential of ZnO-NPs was evaluated for multidrug-resistant (MDR) isolates by MIC. Results All isolates of APEC were resistant to nalidixic acid, whereas 95% were resistant to chloramphenicol and 89% were resistant to streptomycin. Nineteen MDR APEC were found in the present study and the CRISPR-Cas system was detected in all of these MDR isolates. In addition, an increased expression of CRISPR-related genes was observed in the standard strain and MDR isolates of APEC. ZnO-NPs inhibited the growth of resistant isolates. Conclusions The findings showed the presence of the CRISPR-Cas system in MDR strains of APEC, along with the potential of ZnO-NPs for a possible solution to proceed. This highlights the importance of regulating antimicrobial resistance in poultry to reduce potential health consequences.

show abstract

High resistance of fluoroquinolone and macrolide reported in avian pathogenic Escherichia coli isolates from the humid subtropical regions of Pakistan

Cited by 2 publications

References 61 publications

Impact of Prophylactic Antibiotics in Ischemic Stroke Patients on Reduction of Hospital Stay.

Impact of Prophylactic Antibiotics in Ischemic Stroke Patients on Reduction of Hospital Stay.

Potential of ZnO nanoparticles for multi-drug resistant Escherichia coli having CRISPR-Cas from poultry market in Lahore

Contact Info

Product

Resources

About