High relatedness of bioinformatic data and realistic experimental works on the potentials of Fasciola hepatica and F. gigantica cathepsin L1 as a diagnostic and vaccine antigen

Fereig, Ragab M.; Metwally, Samy; El-Alfy, El-Sayed M.; Abdelbaky, Hanan H.; Shanab, Obeid; Omar, Mosaab A.; Alsayeqh, Abdullah F.

doi:10.3389/fpubh.2022.1054502

Cited by 3 publications

(1 citation statement)

References 82 publications

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“…hepatica Cat L protein using the sera of F. hepatica infected or vaccinated cattle in two independent experiments were identified, and these results were confirmed by other studies (35)(36)(37). The study also demonstrated that vaccinated animals with Cat L showed fluke burden reduction with eliciting antibodies (8).…”

Section: Discussionsupporting

confidence: 75%

Can Cathepsin L Epitope Be an Effective Vaccine Candidate Against Fasciola gigantica via Bioinformatics?

Homayouni,

Shaddel,

Ebrahimi

et al. 2023

Ann Mil Health Sci Res

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Background: Fasciolosis is a global parasitic disease caused by liver trematodes such as Fasciola hepatica, affecting various mammals, including sheep, goats, cattle, pigs, and humans worldwide. Cathepsin L (Cat L) found in the life stages of F. gigantica is associated with the pathology of the fluke. Given the detrimental impact of fasciolosis on animal health and production, along with reports of drug resistance and concerns regarding drug residues in animal products and the environment, there has been an increasing emphasis on developing a potent epitope-based vaccine to combat this infection. Objectives: The present study aimed at bioinformatically analyzing the characteristics of Cat L of F. gigantica newly encysted juvenile (FgNEJ) to identify potential epitopes for constructing an effective vaccine against the infection. Methods: The Cat L of FgNEJ was analyzed using several reliable bioinformatics tools. Results: Analysis of the second structure of the Cat L amino acid sequence demonstrated that the alpha-helix, random coil, and extended strand ratios were 77 (23.62%), 164 (50.31%), and 85 (26.07%), respectively. Analysis of B-cell, T-cell, and MHC epitopes predicted on the sequence suggested Cat L of FgNEJ as an ideal target for vaccine design. Conclusions: The current study introduces significant information on Cat L of FgNEJ, as well as some promising epitopes that can be used as strong candidates in studies involved in developing a vaccine against fasciolosis.

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Section: Discussionsupporting

confidence: 75%

Can Cathepsin L Epitope Be an Effective Vaccine Candidate Against Fasciola gigantica via Bioinformatics?

Homayouni,

Shaddel,

Ebrahimi

et al. 2023

Ann Mil Health Sci Res

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Construction and mouse antibody response evaluation of juvenile stage-specific chimeric protein from Fasciola gigantica

Cheukamud,

Chansap,

Rattanasroi

et al. 2024

Veterinary Parasitology

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Developing Multi-epitope Antigen Construct from Immunodominant Proteins for Serological Diagnosis of Chlamydia trachomatis: An In Silico Approach

Ghalamfarsa,

Savardashtaki,

Irajie

et al. 2023

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Background: Chlamydiasis is a widespread bacterial infection in the world. Serological tests are expensive, and in addition, intrinsic antigens can cause cross-reactions and make the diagnosis process difficult. Multi-epitope protein antigens are novel and potential diagnostic markers that have the capability of more accurate and cheaper diagnosis. Therefore, in this study, the main goal is to design a new protein vaccine, including multiple epitopes of B cells with dominant immunity from three proteins named MOMP, ompA and Pgp3D from C. trachomatis Methods: The amino acid sequences were obtained from the UniProt database. The areas with the highest antigenicity were identified using the EMBOSS server. Linear B cell epitopes were determined using BCPRED, ABCpred, and Bepipred servers. Epitopes with the highest antigenicity were connected using the EAAAK linker. Results: Two epitopes from MOMP, two from ompA, and one from Pgp3D were selected. These epitopes were connected to each other with the EAAAK linker. Three residues (0.592), 16 residues (0.76), 36 residues (0.578), and 37 residues (0.734) were obtained from the prediction of the spatial structure of the B cell multiple epitopes designed with ElliPro. Model 1 of RaptorX was selected as the best structure. In this model, the ERRAT quality, ProSA-web z-score, and Verify3D were 83.1169, - 5.17 and 84.62% with PASS score, respectively. Moreover, the Ramachandran plot showed that 86.093% of the amino acid residues were located in the favored region. To achieve the highest level of protein expression, the designed multi-epitope reverse-translated with the Genscript server and was cloned in E. coli. The highest level of expression was achieved, and a CAI score of 0.91 was reported. The gene GC content was 51.98%, and the contribution of low-frequency codons was 0%. Conclusion: The results confirmed that the designed construct could identify C. trachomatis with high sensitivity and specificity in serum samples of patients with chlamydiasis. However, further experimental studies are needed for final confirmation.

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High relatedness of bioinformatic data and realistic experimental works on the potentials of Fasciola hepatica and F. gigantica cathepsin L1 as a diagnostic and vaccine antigen

Cited by 3 publications

References 82 publications

Can Cathepsin L Epitope Be an Effective Vaccine Candidate Against Fasciola gigantica via Bioinformatics?

Can Cathepsin L Epitope Be an Effective Vaccine Candidate Against Fasciola gigantica via Bioinformatics?

Construction and mouse antibody response evaluation of juvenile stage-specific chimeric protein from Fasciola gigantica

Developing Multi-epitope Antigen Construct from Immunodominant Proteins for Serological Diagnosis of Chlamydia trachomatis: An In Silico Approach

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