High regenerative capacity of the liver and irreversible injury of male reproductive system in carbon tetrachloride-induced liver fibrosis rat model

Bubnov, Rostyslav; Drahulian, M. V.; Buchek, P. V.; Gulko, T. P.

doi:10.1007/s13167-017-0115-5

Cited by 22 publications

(13 citation statements)

References 111 publications

(163 reference statements)

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“…b,e P < .05 compared with the DM group. DM: diabetes mellitus; BB: Berberine; TUNEL: terminal deoxynucleotidyl transferase dUTP nick-end labeling Besides, Bubnov RV has proved that reproductive function is largely mutually associated with metabolic health, in particular with liver function,53 and there is evidence that BB can help the liver recover from the antioxidant imbalance, inflammation and apoptosis caused by hyperglycemia, and correct the imbalance in liver metabolic function 54. Therefore, considering the potential protective effect of BB on spermatogenesis of DM rats, we compared the reproductive functions of DM rats in the Berberine treatment and the non-treatment groups in the experiment.…”

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confidence: 99%

Protective effect of Berberine on reproductive function and spermatogenesis in diabetic rats via inhibition of ROS/JAK2/NFκB pathway

et al. 2020

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Background: Diabetes mellitus (DM) induces impairment of male reproductive system and is considered as a key factor that could partially provide an explanation for male infertility. Thus, understanding the mechanism underlying DM-induced infertility will aid in the identification of novel therapeutic stratagems. Objectives: To delineate the role of ROS/JAK2/NFκB pathway in DM-induced low reproductive function and impaired spermatogenesis. Additionally, to investigate the protective effect of monomeric Berberine (BB) that inhibits ROS/JAK2/NFκB pathway, in the pathogenesis of DM-induced infertility.Methods: 12-week-old male Sprague-Dawley rats were divided into four groups: control group, DM group, control plus BB group, and DM plus BB group. Streptozotocin was used to induce DM. After treating the rats with BB for 4 weeks, fertility tests were conducted to investigate the reproductive function, and testis weight along with sperm motility was assessed through microscope. Oxidative stress was evaluated by DHE staining. TUNEL staining was utilized to detect the state of apoptosis. Cell experiments were carried out to define the role of BB in vitro. Immunohistochemistry, immunofluorescence, and Western blotting were employed to measure the protein expression. Results:Our results indicate that the reproductive function of DM rats was low, accompanied by decreased testis weight and sperm motility in addition to the impairment of the seminiferous tubules. However, there was a significant improvement in the reproductive function parameters in the BB-treated DM rats. Subsequently, our data revealed that DM rats produce an increased level of ROS in the testis, which activates JAK2 further activating the NFκB pathway, leading to increased apoptosis and impaired cells in the testicles. However, BB could attenuate the ROS production and abrogate activation of JAK2/NFκB pathway, thus inhibiting the apoptosis in the testicular cells of DM rats.Conclusion: ROS/JAK2/NFκB pathway is involved in the DM-induced low reproductive function and impaired spermatogenesis. BB can play a protective role in

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confidence: 99%

Protective effect of Berberine on reproductive function and spermatogenesis in diabetic rats via inhibition of ROS/JAK2/NFκB pathway

et al. 2020

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“…Additionally, the RR was calculated to estimate the extent of liver regeneration in other experimental models. 21,22 We observed a higher RR 7 days after HSLT in the HMP than SCS group. The cell cycle status is closely associated with the cellular function: rapid cell cycling is necessary for the expansion of a progenitor population, slow cell cycling is required for self-renewal of primitive stem cells, and withdrawal from the cell cycle is needed for various functions of terminally differentiated cells.…”

Section: Discussionmentioning

confidence: 60%

Graft protection of the liver by hypothermic machine perfusion involves recovery of graft regeneration in rats

Jia

Xie

et al. 2019

J Int Med Res

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Objective This study was performed to evaluate the impact and underlying mechanisms of hypothermic machine perfusion (HMP) on half-size liver graft regeneration. Methods Forty rats were randomly assigned to five groups: two in vitro groups (static cold storage [SCS] and HMP) and three in vivo groups (orthotopic liver transplantation, SCS, and HMP). Perfusates and plasma samples were collected for analysis of hepatic enzymes. Liver tissue was obtained for evaluation of histology, immunohistochemistry (Ki67 and proliferating cell nuclear antigen [PCNA]), and the regeneration rate. Cell cycle genes were analyzed by quantitative real-time polymerase chain reaction, and cyclin D1 and cyclin E1 were semiquantified by western blot. Results HMP improved histopathological outcomes and decreased hepatic enzyme release. The expression of Ki67 and PCNA demonstrated a greater proliferation activity in the HMP than SCS group, and the expression of almost all cell cycle genes was elevated following HMP. Western blot results showed higher protein levels of cyclin D1 and cyclin E1 in the HMP than SCS group. Conclusions Our findings suggest for the first time that half-size liver graft protection by HMP involves recovery of graft regeneration.

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“…In experimental models in male rats with hepatic fibrosis induced by CCl 4 , associations with testicular degeneration, hydrocele, fibrosis, scarring, calcifications, hypogonadism, and cryptorchidism have been observed. Then, changes in testicular function, impaired fertility, decreased pregnancy rate, and impaired development of the gonad have been related to liver fibrosis [228]. In aromatase-deficient mice, unable to produce estrogens and suffering from deterioration of the hepatocellular fatty acid β-oxidation, hepatic steatosis develops spontaneously.…”

Section: Preclinical and Clinical Studies On The Effects Of Sex Homentioning

confidence: 99%

Beneficial and Deleterious Effects of Female Sex Hormones, Oral Contraceptives, and Phytoestrogens by Immunomodulation on the Liver

Soria-Jasso

Cariño-Cortés

Muñoz-Pérez

et al. 2019

IJMS

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The liver is considered the laboratory of the human body because of its many metabolic processes. It accomplishes diverse activities as a mixed gland and is in continuous cross-talk with the endocrine system. Not only do hormones from the gastrointestinal tract that participate in digestion regulate the liver functions, but the sex hormones also exert a strong influence on this sexually dimorphic organ, via their receptors expressed in liver, in both health and disease. Besides, the liver modifies the actions of sex hormones through their metabolism and transport proteins. Given the anatomical position and physiological importance of liver, this organ is evidenced as an immune vigilante that mediates the systemic immune response, and, in turn, the immune system regulates the hepatic functions. Such feedback is performed by cytokines. Pro-inflammatory and anti-inflammatory cytokines are strongly involved in hepatic homeostasis and in pathological states; indeed, female sex hormones, oral contraceptives, and phytoestrogens have immunomodulatory effects in the liver and the whole organism. To analyze the complex and interesting beneficial or deleterious effects of these drugs by their immunomodulatory actions in the liver can provide the basis for either their pharmacological use in therapeutic treatments or to avoid their intake in some diseases.

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High regenerative capacity of the liver and irreversible injury of male reproductive system in carbon tetrachloride-induced liver fibrosis rat model

Cited by 22 publications

References 111 publications

Protective effect of Berberine on reproductive function and spermatogenesis in diabetic rats via inhibition of ROS/JAK2/NFκB pathway

Protective effect of Berberine on reproductive function and spermatogenesis in diabetic rats via inhibition of ROS/JAK2/NFκB pathway

Graft protection of the liver by hypothermic machine perfusion involves recovery of graft regeneration in rats

Beneficial and Deleterious Effects of Female Sex Hormones, Oral Contraceptives, and Phytoestrogens by Immunomodulation on the Liver

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