High rate of aneuploidy in luteinized granulosa cells obtained from follicular fluid in women who underwent controlled ovarian hyperstimulation

Kaleli, Semih; Demirel, Gülderen Yanikkaya; Erel, C. Tamer; Şentürk, Levent M.; Topçuoğlu, Ata; İrez, Tülay

doi:10.1016/j.fertnstert.2005.02.040

Cited by 19 publications

(15 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In humans, increasing use of gonadotropins is associated with poor pregnancy rates after IVF (14). Although the exact site of detriment attributable to excess gonadotropins is unclear, adverse influences on the granulosa cells of the developing follicle as well as the oocyte are suggested (22,23). Our data further corroborate an adverse association between excessive gonadotropin exposure during the IVF cycle and the cycle's outcome.…”

Section: Discussionsupporting

confidence: 74%

“…In the absence of this information, we can only hypothesize regarding the mechanisms influenced by excess gonadotropins, such as the developing gamete, the embryo, the endometrium, or the metabolic milieu. Indeed, studies have implicated each of these mechanisms (22)(23)(24).…”

Section: Discussionmentioning

confidence: 99%

“…An increased risk for babies with trisomy 21 is reported in association with maternal FSH levels (6)(7)(8), raising the question whether high circulating levels of gonadotropins plays a causative role. Although in vitro evidence of direct toxic effects of FSH on the mitotic spindle assembly and on chromosomal segregation have been described in both oocytes (22) and granulosa cells (23), these associations are far from substantiated.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Less is more: increased gonadotropin use for ovarian stimulation adversely influences clinical pregnancy and live birth after in vitro fertilization

Pal¹,

Jindal²,

Witt³

et al. 2008

Fertility and Sterility

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 74%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Less is more: increased gonadotropin use for ovarian stimulation adversely influences clinical pregnancy and live birth after in vitro fertilization

Pal¹,

Jindal²,

Witt³

et al. 2008

Fertility and Sterility

View full text Add to dashboard Cite

show abstract

“…Moreover, they demonstrated expression of FSHR in transplantated GCs and thus their functionality. However, use of luteinized GCs as therapeutic cells is questioned since high rate of aneuploidy in GCs was reported in women who underwent in vitro fertilization [22,33]. This limitation could be overcome with isolating and transplanting less differentiated subpopulation of GCs which has not yet undergone luteinization.…”

Section: Resultsmentioning

confidence: 99%

Plasticity of granulosa cells: on the crossroad of stemness and transdifferentiation potential

Dzafic

Štimpfel

Virant‐Klun

2013

J Assist Reprod Genet

View full text Add to dashboard Cite

The ovarian follicle represents the basic functional unit of the ovary and consists of an oocyte, which is surrounded by granulosa cells (GCs). GCs play an important role in the growth and development of the follicle. They are subject to increased attention since it has recently been shown that the subpopulation of GCs within the growing follicle possesses exceptionally plasticity showing stem cell characteristics. In assisted reproduction programs, oocytes are retrieved from patients together with GCs, which are currently discarded daily, but could be an interesting subject to be researched and potentially used in regenerative medicine in the future. Isolated GCs expressed stem cell markers such as OCT-4, NANOG and SOX-2, showed high telomerase activity, and were in vitro differentiated into other cell types, otherwise not present within ovarian follicles. Recently another phenomenon demonstrated in GCs is transdifferentiation, which could explain many ovarian pathological conditions. Possible applications in regenerative medicine are also given.

show abstract

“…When considering the rates of aneuploid granulosa cells found in growing follicles, it is striking that dominant follicles have been shown to have fewer aneuploid granulosa cells [39]–[41]. As high estradiol production is also a feature of the dominant follicle, we hypothesized that estradiol would counter the induction of aberrant mitotic figures by RAP.…”

Section: Resultsmentioning

confidence: 99%

mTOR Controls Ovarian Follicle Growth by Regulating Granulosa Cell Proliferation

Yaba

Kasiman

et al. 2011

PLoS ONE

View full text Add to dashboard Cite

We have shown that inhibition of mTOR in granulosa cells and ovarian follicles results in compromised granulosa proliferation and reduced follicle growth. Further analysis here using spontaneously immortalized rat granulosa cells has revealed that mTOR pathway activity is enhanced during M-phase of the cell cycle. mTOR specific phosphorylation of p70S6 kinase and 4E-BP, and expression of Raptor are all enhanced during M-phase. The predominant effect of mTOR inhibition by the specific inhibitor Rapamycin (RAP) was a dose-responsive arrest in the G1 cell cycle stage. The fraction of granulosa cells that continued to divide in the presence of RAP exhibited a dose-dependent increase in aberrant mitotic figures known as anaphase bridges. Strikingly, estradiol consistently decreased the incidence of aberrant mitotic figures. In mice treated with RAP, the mitotic index was reduced compared to controls, and a similar increase in aberrant mitotic events was noted. RAP injected during a superovulation regime resulted in a dose-dependent reduction in the numbers of eggs ovulated. Implications for the real-time regulation of follicle growth and dominance, including the consequences of increased numbers of aneuploid granulosa cells, are discussed.

show abstract

High rate of aneuploidy in luteinized granulosa cells obtained from follicular fluid in women who underwent controlled ovarian hyperstimulation

Cited by 19 publications

References 8 publications

Less is more: increased gonadotropin use for ovarian stimulation adversely influences clinical pregnancy and live birth after in vitro fertilization

Less is more: increased gonadotropin use for ovarian stimulation adversely influences clinical pregnancy and live birth after in vitro fertilization

Plasticity of granulosa cells: on the crossroad of stemness and transdifferentiation potential

mTOR Controls Ovarian Follicle Growth by Regulating Granulosa Cell Proliferation

Contact Info

Product

Resources

About