High-rate continuous production of lactic acid byLactobacillus rhamnosus in a two-stage membrane cell-recycle bioreactor

Kwon, Soyoung; Yoo, Ik‐Keun; Lee, Woo Gi; Chang, Ho Nam; Chang, Yong Keun

doi:10.1002/1097-0290(20010405)73:1<25::aid-bit1033>3.0.co;2-n

Cited by 115 publications

(49 citation statements)

References 35 publications

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“…N. Chang pursued his single stage continuous HCDC further to improve product titer by adding an extra HCDC unit. Kwon et al [13] operated 2-stage-continuous (SC)-HCDC for lactic acid production and Oh et al [14][15][16] developed depth filter perfusion system for animal cell entrapment and carried out 2SC-HCDC for monoclonal antibody production. The above 2SC-HCDC yielded very encouraging results of increased product titer with maintaining nearly a productivity of 1SC-HCDC.…”

Section: Multi-stage-continuous (Msc)-hcdcsupporting

confidence: 79%

Ho Nam Chang Special Issue: Life of a great biochemical engineer and his life-time contribution to high cell density culture

Lee

2009

Bioprocess Biosyst Eng

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Section: Multi-stage-continuous (Msc)-hcdcsupporting

confidence: 79%

Ho Nam Chang Special Issue: Life of a great biochemical engineer and his life-time contribution to high cell density culture

Lee

2009

Bioprocess Biosyst Eng

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“…Several possibilities exist for evaluating microbial growth rate, but the traditional Monod model was used in this work. (27,39). The results for L. plantarum BPT197 obtained in this study were slightly below the reported values, presumably due to differences in the cultivation conditions.…”

Section: Discussionmentioning

confidence: 99%

Metabolic Engineering of Lactobacillus plantarum for Production of l -Ribulose

Helanto¹,

Kiviharju²,

Leisola³

et al. 2007

Appl Environ Microbiol

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L-Ribulose is a rare and expensive sugar that can be used as a precursor for the production of other rare sugars of high market value such as L-ribose. In this work we describe a production process for L-ribulose using L-arabinose, a common component of polymers of lignocellulosic materials, as the starting material. A ribulokinase-deficient mutant of the heterofermentative lactic acid bacterium Lactobacillus plantarum NCIMB8826 was constructed. Expression of araA, which encodes the critical enzyme L-arabinose isomerase, was repressed by high glucose concentrations in batch cultivations. A fed-batch cultivation strategy was therefore used to maximize L-arabinose isomerase production during growth. Resting cells of the ribulokinasedeficient mutant were used for the production of L-ribulose. The isomerization of L-arabinose to L-ribulose was very unfavorable for L-ribulose formation. However, high L-ribulose yields were obtained by complexing the produced L-ribulose with borate. The process for L-ribulose production in borate buffer by resting cells was optimized using central composite designs. The experiment design suggested that the process has an optimal operation point around an L-arabinose concentration of 100 g liter ؊1 , a borate concentration of 500 mM, and a temperature of 48°C, where the statistical software predicted an initial L-ribulose production rate of 29.1 g liter ؊1 h ؊1 , a best-achievable process productivity of 14.8 g liter ؊1 h ؊1 , and a conversion of L-arabinose to L-ribulose of 0.70 mol mol ؊1 .Despite being a common metabolic intermediate in different organisms, L-ribulose is scarce in nature. This ketopentose has therefore been classified as a rare sugar (3). The markets and interest for rare and unnatural sugars have been growing, especially in the sweetener and pharmaceutical industries (40). Microbial and enzymatic reactions are very suitable for converting D sugars to various L sugars (3).Biotechnological L-ribulose production has been mainly investigated by using ribitol as the raw material. Most efficient L-ribulose production has been obtained by incubating ribitol in the presence of resting cells of acetic acid bacteria (4,14,29). The enzyme catalyzing the oxidation of ribitol to L-ribulose in these bacteria has been shown to be a membrane-bound NAD(P)-independent oxygenase (2). However, the volumes of production of ribitol are small, and it is expensive in pure form.Unlike other L sugars, L-arabinose is fairly abundant in nature. It is a common component of polymers of lignocellulosic materials (20). For example, sugar beet pulp, which is a byproduct of the sugar industry, has been reported to contain considerable amounts of L-arabinose (20%, wt/wt, of the deproteinated mass). L-Arabinose can easily be isolated from the pulp by acid or enzymatic hydrolysis, filtration, and acetone fractionation (41).The L form of ribulose is an intermediate in the pathway for L-arabinose utilization in many bacteria. The L-arabinose taken up by the cells is first isomerized to L-ribulose, which...

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“…Most studies within production of lactic acid have focused on the use of pure substrates such as glucose (9,12) or lactose (5) for the production of lactic acid. The use of natural substrates like starch (4,10,11,18) and cellulose (2,6,16) is economically unfavorable because they are very expensive and also require pretreatment in order to release fermentable sugars.…”

mentioning

confidence: 99%

Utilization of Molasses Sugar for Lactic Acid Production by Lactobacillus delbrueckii subsp. delbrueckii Mutant Uc-3 in Batch Fermentation

Dumbrepatil

Adsul

Chaudhari

et al. 2008

Appl Environ Microbiol

150

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Efficient lactic acid production from cane sugar molasses by Lactobacillus delbrueckii mutant Uc-3 in batch fermentation process is demonstrated. Lactic acid fermentation using molasses was not significantly affected by yeast extract concentrations. The final lactic acid concentration increased with increases of molasses sugar concentrations up to 190 g/liter. The maximum lactic acid concentration of 166 g/liter was obtained at a molasses sugar concentration of 190 g/liter with a productivity of 4.15 g/liter/h. Such a high concentration of lactic acid with high productivity from molasses has not been reported previously, and hence mutant Uc-3 could be a potential candidate for economical production of lactic acid from molasses at a commercial scale.

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High-rate continuous production of lactic acid byLactobacillus rhamnosus in a two-stage membrane cell-recycle bioreactor

Cited by 115 publications

References 35 publications

Ho Nam Chang Special Issue: Life of a great biochemical engineer and his life-time contribution to high cell density culture

Ho Nam Chang Special Issue: Life of a great biochemical engineer and his life-time contribution to high cell density culture

Metabolic Engineering of Lactobacillus plantarum for Production of l -Ribulose

Utilization of Molasses Sugar for Lactic Acid Production by Lactobacillus delbrueckii subsp. delbrueckii Mutant Uc-3 in Batch Fermentation

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