High Quality RNA Retrieved from Samples Obtained by Using LMPC (Laser Microdissection and Pressure Catapulting) Technology

Burgemeister, Renate; Gangnus, Rainer; Haar, B. G. A. ter; Schütze, Karin; Sauer, Ulrich

doi:10.1078/0344-0338-00442

Cited by 38 publications

(20 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Given that molecular analysis requires a high degree of tumor cells purity, we employed the newly described technique of laser capture microdissection 21 under microscopic visualization to select a specific cell population and avoid a possible contamination from adjacent oral mucosa. In two of our cases, a weak amplified band was found.…”

Section: Discussionmentioning

confidence: 99%

Does HPV play a role in the etiopathogenesis of ameloblastoma? An immunohistochemical, in situ hybridization and polymerase chain reaction study of 18 cases using laser capture microdissection

et al. 2005

View full text Add to dashboard Cite

Ameloblastomas are epithelial tumors of odontogenic origin, biologically characterized by local recurrence. Among different etiologic factors, HPV infection has been recently postulated to be somehow involved in ameloblastoma etiopathogenesis. To address this issue, we studied 18 ameloblastomas by means of immunohistochemistry, in situ hybridization (conventional and amplified), polymerase chain reaction and nested-polymerase chain reaction analyses using laser capture microdissection in order to detect the occurrence of HPV in this setting. No evidence of HPV infection was detected by morphological examination, immunohistochemistry, in situ hybridization and conventional polymerase chain reaction, while nestedpolymerase chain reaction showed a weak positive band in two cases. However, the subsequent restriction enzyme analysis carried out from the nested-polymerase chain reaction amplification products of these two samples excluded the presence of HPV subtypes 16,18, 31, 33, 35, 52, and 58. The search for HPV 6 and 11 in the same specimens was also negative. In conclusion, our data do not support an etiopathogenetic evidence for HPV in ameloblastoma.

show abstract

Section: Discussionmentioning

confidence: 99%

Does HPV play a role in the etiopathogenesis of ameloblastoma? An immunohistochemical, in situ hybridization and polymerase chain reaction study of 18 cases using laser capture microdissection

et al. 2005

View full text Add to dashboard Cite

show abstract

“…Fresh-frozen tissue samples from 56 PDAC patients were microdissected to exclude stromal tissue and highly enrich neoplastic cells (Burgemeister et al, 2003). Surgical pancreatic resection specimens were immediately placed on ice and subsequently snapfrozen, then stored at À801C.…”

Section: Microdissection and Qrt -Pcr Analysismentioning

confidence: 99%

The stem cell-associated Hiwi gene in human adenocarcinoma of the pancreas: expression and risk of tumour-related death

et al. 2008

View full text Add to dashboard Cite

Piwi proteins and their interaction with piRNAs have rapidly emerged as important contributors to gene regulation, indicating their crucial function in germline and stem cell development. However, data on the Hiwi 1 (Hiwi) gene, one of the four human Piwi homologues, are still scarce. Therefore, we investigated the Hiwi mRNA expression in microdissected PDAC tissues from patients with ductal adenocarcinoma of the pancreas (PDAC) by quantitative real-time PCR and the protein expression by immunohistochemistry. Elevated levels of Hiwi mRNA transcripts were measured in 40 out of 56 tissues and a positive immunostaining of Hiwi was detected in tumours of 21 out of 78 patients. There was no general impact of elevated Hiwi mRNA transcript levels or protein expression on survival, as tested by multivariate Cox regression and Kaplan -Meier analysis. However, men showed a significantly increased risk for tumour-related death in case of down-or upregulated expression of Hiwi mRNA (relative risk (RR) ¼ 2.78; P ¼ 0.034). In summary, we report the first analysis of Hiwi expression in PDAC and its impact on prognosis. We suggest that alterations in mRNA expression of Hiwi can increase the risk of tumour-related death in male PDAC patients.

show abstract

“…To overcome these limitations, laser capture microdissection and microdissection coupled with laser pressure catapulting (LMPC) have been developed. Combinations with large-scale gene expression analysis have been frequently used for mammalian systems (Emmert-Buck et al, 1996;Luo et al, 1999;Burgemeister et al, 2003). In plants, such applications have been used to monitor gene expression in maize (Zea mays) epidermis, vascular tissues, roots, and shoot apical meristem (Nakazano et al, 2003;Woll et al, 2005;Ohtsu et al, 2007) and Arabidopsis (Arabidopsis thaliana) embryo cells (Casson et al, 2005;Spencer et al, 2006) and siliques (Cai and Lashbrook, 2006).…”

mentioning

confidence: 99%

Different Hormonal Regulation of Cellular Differentiation and Function in Nucellar Projection and Endosperm Transfer Cells: A Microdissection-Based Transcriptome Study of Young Barley Grains

et al. 2008

View full text Add to dashboard Cite

Nucellar projection (NP) and endosperm transfer cells (ETC) are essential tissues in growing barley (Hordeum vulgare) grains, responsible for nutrient transfer from maternal to filial tissues, endosperm/embryo nutrition, and grain development. A laser microdissection pressure catapulting-based transcriptome analysis was established to study NP and ETC separately using a barley 12K macroarray. A major challenge was to isolate high-quality mRNA from preembedded, fixed tissue while maintaining tissue integrity. We show that probes generated from fixed and embedded tissue sections represent largely the transcriptome (.70%) of nonchemically treated and nonamplified references. In NP, the top-down gradient of cellular differentiation is reflected by the expression of C3HC4-type ubiquitin ligases and different histone genes, cell wall biosynthesis and expansin/extensin genes, as well as genes involved in programmed cell death-related proteolysis coupled to nitrogen remobilization, indicating distinct areas simultaneously undergoing mitosis, cell elongation, and disintegration. Activated gene expression related to gibberellin synthesis and function suggests a regulatory role for gibberellins in establishment of the differentiation gradient. Upregulation of plasmalemma-intrinsic protein and tonoplast-intrinsic protein genes indicates involvement in nutrient transfer and/or unloading. In ETC, AP2/EREBP-like transcription factors and ethylene functions are transcriptionally activated, a response possibly coupled to activated defense mechanisms. Transcriptional activation of nucleotide sugar metabolism may be attributed to ascorbate synthesis and/or cell wall biosynthesis. These processes are potentially controlled by trehalose-6-P synthase/phosphatase, as suggested by expression of their respective genes. Up-regulation of amino acid permeases in ETC indicates important roles in active nutrient uptake from the apoplastic space into the endosperm.

show abstract

High Quality RNA Retrieved from Samples Obtained by Using LMPC (Laser Microdissection and Pressure Catapulting) Technology

Cited by 38 publications

References 3 publications

Does HPV play a role in the etiopathogenesis of ameloblastoma? An immunohistochemical, in situ hybridization and polymerase chain reaction study of 18 cases using laser capture microdissection

Does HPV play a role in the etiopathogenesis of ameloblastoma? An immunohistochemical, in situ hybridization and polymerase chain reaction study of 18 cases using laser capture microdissection

The stem cell-associated Hiwi gene in human adenocarcinoma of the pancreas: expression and risk of tumour-related death

Different Hormonal Regulation of Cellular Differentiation and Function in Nucellar Projection and Endosperm Transfer Cells: A Microdissection-Based Transcriptome Study of Young Barley Grains

Contact Info

Product

Resources

About