High-Quality Genomic DNA from Human Whole Blood and Mononuclear Cells

Malferrari, Giulia; Monferini, E.; Blasio, Pasquale De; Diaferia, Giuseppe R.; Saltini, Giuliana; Vecchio, Emanuela Del; Rossi-Bernardi, L.; Biunno, Ida

doi:10.2144/02336bm09

Cited by 35 publications

(23 citation statements)

References 9 publications

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“…The total soil DNA was diluted tenfold and added to the reaction mixture in an amount of 1 μl, or about 1-10 ng. The reaction products were resolved by agar gel electrophoresis, and the amplified fragment was cut out of the gel and extracted as described [25]. The resulting aliquot (100-200 ng) was treated with HaeIII endonuclease, precipitated with ethanol, dis solved in the SLS reagent supplemented with a DNA size standard kit (Beckman Coulter), and resolved by capillary electrophoresis with fluorescent detection in a CEQ8000 sequencer.…”

Section: Objects and Methodsmentioning

confidence: 99%

Analysis of the structure of microbial community in soils with different degrees of salinization using T-RFLP and real-time PCR techniques

et al. 2012

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Molecular methods were used to study variation in the taxonomic structure of bacterial, archaeal, and fungal communities in soil samples taken along a salinity gradient from a solonchak in the vicinity of Lake Akkol' (Shingirlau, Kazakhstan). Soils from arable fields located 195 km from the solonchak served as the control. Total DNA was isolated from every sample and analyzed by T RFLP and real time PCR. Saliniza tion was found to be the main ecological factor determining the structure of soil microbial community in the study region. The values of Simpson's index characterizing the diversity of this community proved to be sim ilar in all the samples, which, however, significantly differed in the taxonomic composition of microorgan isms. A significantly increased content of archaea was revealed in the sample with the highest salinity. The results of this study show that the structure of soil microbial community reflects specific features of a given soil and can be used as an indicator of its ecological state.

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Section: Objects and Methodsmentioning

confidence: 99%

Analysis of the structure of microbial community in soils with different degrees of salinization using T-RFLP and real-time PCR techniques

et al. 2012

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“…Genomic DNA was isolated semi-automatically from peripheral blood cells by the use of a previously described protocol adapted to a robotic liquid handling platform (Multiprobe II, Perkin Elmer) [16]. A TaqMan 'assay-ondemand' (No.…”

Section: Genotypingmentioning

confidence: 99%

Platelet reactivity and clopidogrel resistance are associated with the H2 haplotype of the P2Y12-ADP receptor gene

Staritz¹,

Kurz²,

Stoll³

et al. 2009

International Journal of Cardiology

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“…DNA extraction from the paraffin-embedded cores required initial deparaffinization and rehydration by incubating in decreasing concentrations of alcohol and final digestion with Proteinase K. The silica-based procedure 16 was used to extract the DNA from two deparaffinized cores. Briefly, after rehydration the cores were incubated in 3 M guanidine thiocyanate, 20.0 mM EDTA pH 8, 10.0 mM TrisHCl pH 6.8, 10 mg/mL DTT, 40 mg/mL Triton X-100, and 40 mg buffered silica matrix for 3 min at room temperature, and then centrifuged at 135 g for 60 sec at room temperature.…”

Section: Nucleic Acid Extractionmentioning

confidence: 99%

Evaluation of Cross-Sample Contamination in Tissue Microarrays by Polymerase Chain Reaction

D'Urso

Spada

Tramonte

et al. 2015

Biopreservation and Biobanking

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In the past decade, the popularity and power of Tissue Microarray (TMA) technology has increased since it provides a method to detect diagnostic and prognostic markers in an array of clinical tissue specimens collected for translational research. TMAs allow for rapid and cost-effective analysis of hundreds of molecular markers at the nucleic acid and protein levels. This technology is particularly useful in the realization of the Human Protein Atlas Project, since it aims to create a reference database of non-redundant human proteins. In this context, it is important to assure the lack of cross-sample contamination due to the repeated use of the same needle in consecutive coring. Here we show that carry-over contamination from one tissue core to another does not occur, reinforcing the accuracy of the TMA technology in the simultaneous testing of multiple bio-samples.

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High-Quality Genomic DNA from Human Whole Blood and Mononuclear Cells

Abstract: Etest and a microbroth dilution system (Sceptor) to a reference agar dilution method for susceptibility testing of Bilophila wadsworthia.

Cited by 35 publications

References 9 publications

Analysis of the structure of microbial community in soils with different degrees of salinization using T-RFLP and real-time PCR techniques

Analysis of the structure of microbial community in soils with different degrees of salinization using T-RFLP and real-time PCR techniques

Platelet reactivity and clopidogrel resistance are associated with the H2 haplotype of the P2Y12-ADP receptor gene

Evaluation of Cross-Sample Contamination in Tissue Microarrays by Polymerase Chain Reaction

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