High-quality draft genome sequences of five anaerobic oral bacteria and description of Peptoanaerobacter stomatis gen. nov., sp. nov., a new member of the family Peptostreptococcaceae

Sizova, Maria V.; Chilaka, Amanda C; Earl, Ashlee M.; Doerfert, Sebastian N.; Muller, Paul; Torralba, Manolito; McCorrison, Jamison; Durkin, A. Scott; Nelson, Karen E.; Epstein, Slava S.

doi:10.1186/s40793-015-0027-8

Cited by 22 publications

(25 citation statements)

References 39 publications

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“…Species such as P. stomatis that are detected with higher frequency in periodontal disease sites than in healthy sites are emerging as periodontal pathogens (10,12,14).…”

Section: Discussionmentioning

confidence: 99%

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Peptoanaerobacter stomatis Primes Human Neutrophils and Induces Granule Exocytosis

et al. 2017

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Peptoanaerobacter stomatis is a newly appreciated taxon associated with periodontal diseases; however, little is known about the organism's pathogenic potential or its interaction with the host immune response. Neutrophils are the most abundant innate immune cell present in the gingival tissue and function to constrain the oral microbial challenge. However, some periodontal pathogens have developed strategies to evade phagocytosis and killing by neutrophils. Therefore, to begin to understand the role of P. stomatis in periodontitis, we studied its interactions with human neutrophils. Our data showed that after 30 min of incubation, neutrophils failed to engulf P. stomatis efficiently; however, when P. stomatis was internalized, it was promptly eradicated. P. stomatis challenge induced a robust intracellular respiratory burst; however, this response did not contribute to bacterial killing. Minimal superoxide release was observed by direct bacterial challenge; however, P. stomatis significantly increased N-formyl-methionyl-leucyl phenylalanine (fMLF)-stimulated superoxide release to an extent similar to that of cells primed with tumor necrosis factor alpha (TNF-␣). When neutrophils were challenged with P. stomatis, 52% of the bacterium-containing phagosomes were enriched for the specific granule marker lactoferrin and 82% with the azurophil granule marker elastase. P. stomatis challenge stimulated exocytosis of the four neutrophil granule subtypes. Moreover, P. stomatis susceptibility to extracellular killing could be attributed to the exocytosis of antimicrobial components present in neutrophil granules. Priming neutrophils for an enhanced respiratory burst together with promoting granule content release could contribute to the chronic inflammation and tissue destruction that characterize periodontal diseases.

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“…Species such as P. stomatis that are detected with higher frequency in periodontal disease sites than in healthy sites are emerging as periodontal pathogens (10,12,14).…”

Section: Discussionmentioning

confidence: 99%

“…P. stomatis is a Gram-positive obligate anaerobic rod with round ends, with a diameter of 0.5 to 0.7 m and a length of 1.0 to 2.3 m, often forming chains (12), and highly motile due to peritrichous flagella (14). In addition to periodontitis, P. stomatis is associated with dentoalveolar abscesses and endodontic infections (14).…”

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confidence: 99%

Peptoanaerobacter stomatis Primes Human Neutrophils and Induces Granule Exocytosis

et al. 2017

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“…Peptoanaerobacter stomatis is a newly appreciated pathogen that has been recognized to be present in oral biofilms in patients with periodontal diseases (Kumar et al, 2005;Murphy & Frick, 2013;Sizova et al, 2015). It was proposed by Sizova et al (2015) as a novel genus and species within the family of Peptostreptococcaceae, and its bacterial strains ACC19a, CM2, CM5, and OBRC8 represent the first known cultivable members of the human oral taxon…”

Section: Peptoanaerobacter Stomatis and Periodontal Diseasesmentioning

confidence: 99%

“…It was proposed by Sizova et al (2015) as a novel genus and species within the family of Peptostreptococcaceae, and its bacterial strains ACC19a, CM2, CM5, and OBRC8 represent the first known cultivable members of the human oral taxon…”

Section: Peptoanaerobacter Stomatis and Periodontal Diseasesmentioning

confidence: 99%

“…The P. stomatis genome is 2,541,543-bp long and contains 2,277 proteincoding and 54 RNA genes; it has a 30.37 mol % G + C content (Sizova et al, 2015). Based on strains isolated from the human oral cavity, P. stomatis cells can be described as Gram-positive obligate anaerobic rods, about 1.2 -2.5 µm long and 0.4 -0.8 µm wide, often occurring in pairs or short chains (Sizova et al, 2015).…”

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Peptoanaerobacter stomatis interactions with neutrophils.

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Suppuration as diagnostic criterium of peri‐implantitis

Monje

Vera

Muñoz-Sanz

et al. 2020

Journal of Periodontology

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Background Suppuration (SUP) as a diagnostic parameter for monitoring dental implants is not yet well understood. The retrospective clinical and radiographic study was therefore performed to investigate the patient, implant, and site characteristics among individuals exhibiting SUP. Methods Demographic characteristics and clinical parameters were recorded. Radiographic features were analyzed using cone‐beam computed tomography. Peri‐implantitis was defined based on the consensus report of Workgroup 4 of the 2017 World Workshop on the Classification of Periodontal and Peri‐Implant Diseases and Conditions: probing depth (PD) ≥6 mm, presence of bleeding and/or SUP on gentle probing, and radiographic marginal bone loss (MBL) ≥3 mm. SUP was graded according to profuseness (dot versus line/drop) and time after probing (≥15 seconds versus <15 seconds after probing versus spontaneous). Simple binary logistic regression models were estimated using generalized estimation equations to explain the probability of SUP based on demographic, clinical, and radiographic variables. Results A total of 111 eligible patients (nimplants = 501) were assessed. Of them, 57 (nimplants = 334) were diagnosed with peri‐implantitis according to the established case definition, and of these individuals, 31 (nimplants = 96) presented SUP. Therefore, the prevalence of SUP was 27.92% in the total sample size and 54.38% in peri‐implantitis patients. Overall, 28.74% implants displayed SUP within patients with peri‐implantitis. SUP was more frequently found at buccal sites (51%) and proved less prevalent at mesio‐lingual sites (16.7%). Defect morphology (OR = 6.59; P = 0.004), PD (OR = 1.63; P = 0.024), and MBL (OR = 1.35; P = 0.010) were significantly associated with the presence of SUP. Likewise, defect morphology (P = 0.02), PD (P = 0.003), and MBL (P = 0.01) were significantly correlated with the grade of SUP. Conclusion The presence and grade of SUP are associated with peri‐implant bone loss, probing depth, and defect morphology in patients with peri‐implantitis.

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High-quality draft genome sequences of five anaerobic oral bacteria and description of Peptoanaerobacter stomatis gen. nov., sp. nov., a new member of the family Peptostreptococcaceae

Cited by 22 publications

References 39 publications

Peptoanaerobacter stomatis Primes Human Neutrophils and Induces Granule Exocytosis

Peptoanaerobacter stomatis Primes Human Neutrophils and Induces Granule Exocytosis

Peptoanaerobacter stomatis interactions with neutrophils.

Suppuration as diagnostic criterium of peri‐implantitis

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