High-Quality Draft Genome Sequences of Two
            <i>Xanthomonas</i>
            Pathotype Strains Infecting Aroid Plants

Robène, Isabelle; Bolot, Stéphanie; Pruvost, Olivier; Arlat, Matthieu; Noël, Laurent; Carrère, Sébastien; Martin, Jacques; Koebnik, Ralf; Gagnevin, Lionel

doi:10.1128/genomea.00902-16

Cited by 3 publications

(4 citation statements)

References 11 publications

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“…oryzicola BLS256 Oryza sativa Philippines, 1984 1 4,831,739 64.05 AAQN01000001 Bogdanove et al, 2011 [ 83 ] axdieff695 X. phaseoli pv. dieffenbachiae LMG695 Anthurium andreanum Brazil, 1965 1 5,037,357 64.88 GCA_001564415.1 Robène et al, 2016 [ 96 ] axmaniC151 X. phaseoli pv. manihotis CIO151 Manihot sp.…”

Section: Resultsmentioning

confidence: 99%

“…CFO16 Iran, 2006 1 5,085,978 64.89 GCA_900234445 this study axsyng9055 X. phaseoli pv. syngonii (NF1) LMG9055 Syngonium podophyllum na 6 5,010,905 64.82 GCA_001640215.1 Robène et al, 2016 [ 96 ] saccha4393 X. sacchari NCPPB4393 Musa sp. Tanzania, 2007 470 4,955,099 69.03 AGDB00000000 Studholme et al, 2011 [ 98 ] cereal2541 X. translucens pv.…”

Section: Resultsmentioning

confidence: 99%

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Horizontal gene transfer plays a major role in the pathological convergence of Xanthomonas lineages on common bean

et al. 2018

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BackgroundHost specialization is a hallmark of numerous plant pathogens including bacteria, fungi, oomycetes and viruses. Yet, the molecular and evolutionary bases of host specificity are poorly understood. In some cases, pathological convergence is observed for individuals belonging to distant phylogenetic clades. This is the case for Xanthomonas strains responsible for common bacterial blight of bean, spread across four genetic lineages. All the strains from these four lineages converged for pathogenicity on common bean, implying possible gene convergences and/or sharing of a common arsenal of genes conferring the ability to infect common bean.ResultsTo search for genes involved in common bean specificity, we used a combination of whole-genome analyses without a priori, including a genome scan based on k-mer search. Analysis of 72 genomes from a collection of Xanthomonas pathovars unveiled 115 genes bearing DNA sequences specific to strains responsible for common bacterial blight, including 20 genes located on a plasmid. Of these 115 genes, 88 were involved in successive events of horizontal gene transfers among the four genetic lineages, and 44 contained nonsynonymous polymorphisms unique to the causal agents of common bacterial blight.ConclusionsOur study revealed that host specificity of common bacterial blight agents is associated with a combination of horizontal transfers of genes, and highlights the role of plasmids in these horizontal transfers.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-4975-4) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Horizontal gene transfer plays a major role in the pathological convergence of Xanthomonas lineages on common bean

et al. 2018

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“…The other group, with the exception of X. axonopodis pv. dieffenbachiae LMG 695 (Xad LMG695), a pathogen of monocot Ariod plants (Robène et al, 2016), consists of dicot plant parasites, including X. axonopodis pv. phaseoli (XapCFBP6982, pathogen of bean (Gent et al, 2005), X. perforans (Xp91-118, pathogen of Solanacearum plant (Itako et al, 2015), X. euvesicatoria LMG930 (Xe LMG930, pathogen of pepper Vancheva et al, 2018), X. campestris pv.…”

Section: Resultsmentioning

confidence: 99%

Mutagenesis of PhaR, a Regulator Gene of Polyhydroxyalkanoate Biosynthesis of Xanthomonas oryzae pv. oryzae Caused Pleiotropic Phenotype Changes

Long

Song

et al. 2018

Front. Microbiol.

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Polyhydroxyalkanoates (PHAs) are intracellular carbon and energy storage materials produced in various microorganisms under nutrient-limited conditions. PhaR is a regulatory protein involved in PHA synthesis. Xanthomonas oryzae pv. oryzae (Xoo) is one of the most important bacterial pathogens in rice and has PHA biosynthesis genes in its genome, but the biological function of phaR in Xoo is unknown. In this study, we investigated the effects of the mutagenesis of phaR gene in Xoo strain PXO99A. Compared to the wildtype, the PhaR gene knock-out mutant PXO99ΔphaR was hypermotile and showed decreased growth rates in both rich and limited nutrient media. PXO99ΔphaR also showed almost 75% decrease in extracellular polysaccharide (EPS) production. When inoculated in rice leaves by leaf-clipping method, PXO99ΔphaR displayed reduced virulence in terms of lesion length and bacterial multiplication compared with the wildtype strain. PXO99ΔphaR also showed enhanced hypersensitive response (HR) induction in the leaves of non-host Nicotiana benthamiana with elevated hpa1 gene expression. Introduction of a cosmid containing the phaR coding sequence restored the phenotypes of the mutant to those of the wildtype strain. These results suggest that PhaR gene is an important gene that affects multiple bacterial characteristics, including EPS production, growth rate, defense response induced harpin production and motility, related to its virulence in plant.

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“…The strain isolated from Syngonium (Xps) had more T3E genes than the strain isolated from Anthurium (Xpd), which was also observed by Robène et al . () for LMG 695 from Anthurium and LMG 9055 from Syngonium . Moreover, the XopJ5 , XopAF and XopAJ genes, which were only present in Xe, had low GC content ( XopJ5 ( AvrXccB ) 59%, XopAF ( AvrXv3 ) 53%, XopAJ ( AvrRxo1 ) 50%) compared to an average genome GC content of 65%.…”

Section: Resultsmentioning

confidence: 99%

Pathogenicity and virulence gene content of Xanthomonas strains infecting Araceae, formerly known as Xanthomonas axonopodis pv. dieffenbachiae

Constantin¹,

Haegeman²,

Vaerenbergh³

et al. 2017

Plant Pathology

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Bacterial leaf blight of aroids is caused by a heterogeneous group of xanthomonads listed as Xanthomonas axonopodis pv. dieffenbachiae (Xad) on the EPPO A2 quarantine list. Recently, Xad strains were shown not to belong to X. axonopodis but to the species X. citri, X. phaseoli and X. euvesicatoria. Here, to verify the pathovar designation, 11 representative strains were tested for pathogenicity on six aroid genera. They had overlapping host ranges and only the strain isolated from Syngonium showed host specificity. The X. citri strains, isolated from various hosts, showed dissimilarity in virulence to the tested aroid genera. The X. phaseoli strains, isolated from Anthurium and Syngonium, were generally more virulent and, additionally, induced systemic infections. The X. euvesicatoria strains, isolated from Philodendron, were scored as not pathogenic on the tested aroids. Four representative strains were genome sequenced and showed a variable virulence-associated gene content. Pathogenicity to aroids was correlated with the presence of three specific T3 effector genes and with a T6SS gene sequence. Together, the phylogenetic and pathogenic differentiation among Xad strains justifies the installation of three pathovar epithets for the pathogens on aroids: X. phaseoli pv. dieffenbachiae comb. nov. for the strains isolated from Anthurium; X. phaseoli pv. syngonii comb. nov. for the strain isolated from Syngonium; and X. citri pv. aracearum comb. nov. for the strains isolated from Aglaonema, Xanthosoma and Dieffenbachia. It is proposed that phytosanitary regulations for xanthomonads on aroids are restricted to these three pathovars.

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High-Quality Draft Genome Sequences of Two Xanthomonas Pathotype Strains Infecting Aroid Plants

Cited by 3 publications

References 11 publications

Horizontal gene transfer plays a major role in the pathological convergence of Xanthomonas lineages on common bean

Horizontal gene transfer plays a major role in the pathological convergence of Xanthomonas lineages on common bean

Mutagenesis of PhaR, a Regulator Gene of Polyhydroxyalkanoate Biosynthesis of Xanthomonas oryzae pv. oryzae Caused Pleiotropic Phenotype Changes

Pathogenicity and virulence gene content of Xanthomonas strains infecting Araceae, formerly known as Xanthomonas axonopodis pv. dieffenbachiae

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