High-Quality Draft Genome Sequence of Fusarium oxysporum f. sp.
            <i>cubense</i>
            Strain 160527, a Causal Agent of Panama Disease

Asai, Shuji; Ayukawa, Yu; Gan, Pamela; Masuda, Sachiko; Komatsu, Ken; Shirasu, Ken; Arie, Tsutomu

doi:10.1128/mra.00654-19

Cited by 19 publications

(14 citation statements)

References 15 publications

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“…militaris [48] and Cor. Cicadae [49]), and three Fusarium (F. solani (https://www.ncbi.nlm.nih.gov/genome/?term=Fusarium+solani), F. oxysporum [50], and F. graminearum [51]). A total of 8957 ortholog families (groups), comprising 10,983 proteins, were produced, as well as 166 orphans that showed no homology with any other proteins in the dataset.…”

Section: Comparative and Evolutionary Analysismentioning

confidence: 99%

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Genomic Features of Cladobotryum dendroides, Which Causes Cobweb Disease in Edible Mushrooms, and Identification of Genes Related to Pathogenicity and Mycoparasitism

Liu

Peng

et al. 2020

Pathogens

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Cladobotryum dendroides, which causes cobweb disease in edible mushrooms, is one of the major fungal pathogens. Our previous studies focused on the genetic and morphological characterization of this fungus, as well as its pathogenicity and the identification of appropriate fungicides. However, little is known about the genome characters, pathogenic genes, and molecular pathogenic mechanisms of C. dendroides. Herein, we reported a high-quality de novo genomic sequence of C. dendroides and compared it with closely-related fungi. The assembled C. dendroides genome was 36.69 Mb, consisting of eight contigs, with an N50 of 4.76 Mb. This genome was similar in size to that of C. protrusum, and shared highly conserved syntenic blocks and a few inversions with C. protrusum. Phylogenetic analysis revealed that, within the Hypocreaceae, Cladobotryum was closer to Mycogone than to Trichoderma, which is consistent with phenotypic evidence. A significant number of the predicted expanded gene families were strongly associated with pathogenicity, virulence, and adaptation. Our findings will be instrumental for the understanding of fungi–fungi interactions, and for exploring efficient management strategies to control cobweb disease.

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Section: Comparative and Evolutionary Analysismentioning

confidence: 99%

“…The homology approach was based on representative reference genomes, including those of Cor. militaris [48], T. reesei [26], C. protrusum [17], F. oxysporum [50], and F. graminearum [51]. De novo predictions were performed using Augustus [59] and GlimmerHMM [60].…”

Section: Gene Prediction and Genome Annotationmentioning

confidence: 99%

Genomic Features of Cladobotryum dendroides, Which Causes Cobweb Disease in Edible Mushrooms, and Identification of Genes Related to Pathogenicity and Mycoparasitism

Liu

Peng

et al. 2020

Pathogens

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“…Among the six hits obtained, the race 1 isolate, Fusarium oxysporum f. sp. cubense strain 160527 gave 100% query cover with a 99.91% identity 29 . Ninety five percent identity was seen when Pxy sequence was aligned with race 1 (Fusarium oxysporum f. sp.…”

Section: Resultsmentioning

confidence: 99%

Functional characterization of an inducible bidirectional promoter from Fusarium oxysporum f. sp. cubense

Dash

Gurdaswani

D’Souza

et al. 2020

Sci Rep

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Bidirectional promoters (BDPs) are regulatory DNA sequences (~1000 bp long) intervening two genes arranged on opposite strands with their 5′ ends in close proximity. these genes are mostly co-expressed; but, instances of anti-correlation and independent transcription have been observed. In fungal systems, BDPs have shown to provide an improved genetic circuit by assembling and regulating transcription of different genes of a common metabolic pathway. We have identified an intergenic region (1063 bp) from the genome of Fusarium oxysporum f. sp. cubense (Foc), a banana root pathogen. this intergenic region regulates the expression of a gene pair required for the breakdown of hemicellulose. For characterization, it was cloned into pCSN44 vector backbone between two reporter genes, namely β-glucuronidase (GUS) and enhanced green fluorescent protein (EGFP). The newly formed vector was transformed into Foc and tested for its bidirectional expression activity. Using histochemical staining and fluorescence microscopy, the kinetics for both, GUS and EGFP expression were tested under different growth conditions respectively. The activity was differentially regulated by inducers such as xylan, arabinogalactan and pectin. This is the first report on the isolation of the intergenic region with inducible bidirectional promoter activity from Fusarium. characterization of such BDPs will find applications in genetic engineering, metabolic engineering and synthetic biology using fungal systems.

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“…cubense) genome, suggesting that it has become easy to to understand the essence of chromosomes. 32) I predict that such innovation in research techniques will further accelerate Fusarium studies and reveal biologically significant findings.…”

Section: Discussionmentioning

confidence: 99%

<i>Fusarium</i> diseases of cultivated plants, control, diagnosis, and molecular and genetic studies

Arie

2019

J. Pestic. Sci.

Self Cite

101

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Fusarium diseases are significant hindrances to food plant production and are very difficult to control, especially soilborne diseases caused by F. oxysporum. First I outline the Fusarium diseases and introduce examples of the recent outbreak of Fusarium diseases in Japan. Then I summarize my studies on (1) the control of Fusarium diseases by biological agents and by inducing resistance to diseases in plants, (2) the specific detection of forms and races in F. oxysporum using immunological measures and molecular measures based on phylogeny and pathogenicity-determining genes, and (3) molecular and genetic studies on Fusarium diseases, including evolutionary, genetic, and genomic analyses of the emergence and divergence of forms and races in F. oxysporum.

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High-Quality Draft Genome Sequence of Fusarium oxysporum f. sp. cubense Strain 160527, a Causal Agent of Panama Disease

Cited by 19 publications

References 15 publications

Genomic Features of Cladobotryum dendroides, Which Causes Cobweb Disease in Edible Mushrooms, and Identification of Genes Related to Pathogenicity and Mycoparasitism

Genomic Features of Cladobotryum dendroides, Which Causes Cobweb Disease in Edible Mushrooms, and Identification of Genes Related to Pathogenicity and Mycoparasitism

Functional characterization of an inducible bidirectional promoter from Fusarium oxysporum f. sp. cubense

<i>Fusarium</i> diseases of cultivated plants, control, diagnosis, and molecular and genetic studies

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