High-quality assembly of Dermatophagoides pteronyssinus genome and transcriptome reveals a wide range of novel allergens

Liu, Xiaoyu; Yang, Kevin Yi; Wang, Mingqiang; Kwok, Jamie Sui-Lam; Zeng, Xi; Yang, Zhiyuan; Xiao, Xiaojun; Lau, Carol; Li, Ying; Huang, Zhiming; Ba, Jin-ge; Yim, Aldrin Kay-Yuen; Ouyang, Chunyan; Ngai, Sai-Ming; Chan, Ting‐Fung; Leung, Elaine Lai‐Han; Liu, Liang; Liu, Zhigang; Tsui, Stephen Kwok‐Wing

doi:10.1016/j.jaci.2017.11.038

Cited by 39 publications

(63 citation statements)

References 38 publications

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“…The most highly expressed gene in the cluster showed very similar levels of expression in both larvae and tritonymphs and shares significant homology with a novel gene of unknown function from the HDM D. farinae [48]. The protein encoded by this gene was termed Dermatophagoides farinae most abundant protein 2 (DFP2) and as yet remains uncharacterised functionally [48]. Two further highly expressed genes in this cluster encode P. ovis homologues of an ADP/ATP translocase (psovi43g01550) and a chaperonin containing TCP1 complex protein (CCT1) (psovi283g00230).…”

Section: Genes Enriched In P Ovis Larvae (L) -Clustermentioning

confidence: 96%

“…The cluster of larvae-enriched genes represents one of the smallest single-stage clusters with only 112 genes. The most highly expressed gene in the cluster showed very similar levels of expression in both larvae and tritonymphs and shares significant homology with a novel gene of unknown function from the HDM D. farinae [48]. The protein encoded by this gene was termed Dermatophagoides farinae most abundant protein 2 (DFP2) and as yet remains uncharacterised functionally [48].…”

Section: Genes Enriched In P Ovis Larvae (L) -Clustermentioning

confidence: 99%

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A genomic analysis and transcriptomic atlas of gene expression inPsoroptes ovisreveals feeding- and stage-specific patterns of allergen expression

Burgess

Marr

Bartley

et al. 2019

Preprint

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Psoroptic mange, caused by infestation with the ectoparasitic mite, Psoroptes ovis, is highly contagious, resulting in intense pruritus and represents a major welfare and economic concern for the livestock industry Worldwide. Control relies on injectable endectocides and organophosphate dips, but concerns over residues, environmental contamination, and the development of resistance threaten the sustainability of this approach, highlighting interest in alternative control methods. However, development of vaccines and identification of chemotherapeutic targets is hampered by the lack of P. ovis transcriptomic and genomic resources. Building on the recent publication of the P. ovis draft genome, here we present a genomic analysis and transcriptomic atlas of gene expression in P. ovis revealing feeding-and stage-specific patterns of gene expression, including novel multigene families and allergens. Network-based clustering revealed 14 gene clusters demonstrating either single-or multi-stage specific gene expression patterns, with 3,075 female-specific, 890 male-specific and 112, 217 and 526 transcripts showing larval, protonymph and tritonymph specific-expression, respectively. Detailed analysis of P. ovis allergens revealed stage-specific patterns of allergen gene expression, many of which were also enriched in "fed" mites and tritonymphs, highlighting an important feeding-related allergenicity in this developmental stage. Pair-wise analysis of differential expression between life-cycle stages identified patterns of sex-biased gene expression and also identified novel P. ovis multigene families including known allergens and novel genes with high levels of stage-specific expression. The genomic and transcriptomic atlas described here represents a unique resource for the acarid-research community, whilst the OrcAE platform makes this freely available, facilitating further community-led curation of the draft P. ovis genome.3

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Section: Genes Enriched In P Ovis Larvae (L) -Clustermentioning

confidence: 96%

Section: Genes Enriched In P Ovis Larvae (L) -Clustermentioning

confidence: 99%

A genomic analysis and transcriptomic atlas of gene expression inPsoroptes ovisreveals feeding- and stage-specific patterns of allergen expression

Burgess

Marr

Bartley

et al. 2019

Preprint

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“…In our previous studies, we have reported the genomes and transcriptomes of DPT. 7 In this study, we identified and assigned the putative functions of miRNAs in DPT. We also conducted a comparative study with the miRNA in other arthropods to determine whether our identified miRNAs display critical and specific functions in HDM.…”

Section: Identification Functional Annotation and Stability Analysimentioning

confidence: 99%

“…The genome of DPT has been reported previously. 7 The high-quality raw reads (SRA accession: PRJNA577524) were mapped to the genome ( Figure S2). Other types of small noncoding RNAs were removed.…”

Section: Identification Functional Annotation and Stability Analysimentioning

confidence: 99%

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Identification, functional annotation, and stability analysis of miRNA in Dermatophagoides pteronyssinus

Yang

Wan

Liu

et al. 2019

Allergy

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To the Editor, House dust mite (HDM) is one of the major allergen sources worldwide which causes up to 85% of respiratory allergic diseases. 1 This species was regarded as the most prevalent source of indoor allergens in the United States and Europe. HDM is a popular name of the taxonomic genus Dermatophagoides that include several species, such as Dermatophagoides pteronyssinus (DPT) and Dermatophagoides farinae (DFA). The sensitization of HDM is strongly associated with asthma. 2 MicroRNAs (miRNAs) are small RNAs that play an important role in post-transcriptional regulation of different biological processes, such as cell differentiation and cell death. There is emerging evidence showing a critical role of miRNAs in the regulation of inflammation and allergic airway diseases. 3 Previous studies showed that

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Indoor Airborne Insect Allergens

Kausar,

Anwar,

Najm

et al. 2024

Airborne Biocontaminants and Their Impact on Human Health

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High-quality assembly of Dermatophagoides pteronyssinus genome and transcriptome reveals a wide range of novel allergens

Cited by 39 publications

References 38 publications

A genomic analysis and transcriptomic atlas of gene expression inPsoroptes ovisreveals feeding- and stage-specific patterns of allergen expression

A genomic analysis and transcriptomic atlas of gene expression inPsoroptes ovisreveals feeding- and stage-specific patterns of allergen expression

Identification, functional annotation, and stability analysis of miRNA in Dermatophagoides pteronyssinus

Indoor Airborne Insect Allergens

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