High purity microfluidic sorting and in situ inactivation of circulating tumor cells based on multifunctional magnetic composites

Xu, Hongwei; Dong, Biao; Xu, Shihan; Xu, Sai; Sun, Xueke; Sun, Jiao; Yang, Yudan; Xu, Lin; Bai, Xue; Zhang, Shuang; Yin, Ze

doi:10.1016/j.biomaterials.2017.05.035

Cited by 33 publications

(32 citation statements)

References 40 publications

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“…In fact, the capability of capturing and isolating biological entities at the microscale level, combining LOC and MNP technologies, creates the potential for cost effective, portable, and disposable point‐of‐care systems to monitor and detect various diseases. In particular, the detection and isolation of circulating tumor cells (CTCs) play a key role in the diagnosis and prognosis of cancer, while the high capture efficiency and purity of CTCs are difficult to reach simultaneously among the various conventional isolation methods due to the small throughput, incapability to release captured cells, and dependence on expensive instrumentation for enrichment or subsequent characterization . In an attempt to take a step forward to overcome the aforementioned limitation, an inverted microchip integrating silicon nanowires (SiNW) and multifunctional magnetic nanocomposites based on anti‐EpCAM‐Fe 3 O 4 @C6/Ce6@silane (anti‐epithelial cell adhesion molecule (EpCAM)) was designed to enhanced capture efficiency and purity of CTCs .…”

Section: Representative Biomedical Applicationsmentioning

confidence: 99%

“…In particular, the detection and isolation of circulating tumor cells (CTCs) play a key role in the diagnosis and prognosis of cancer, while the high capture efficiency and purity of CTCs are difficult to reach simultaneously among the various conventional isolation methods due to the small throughput, incapability to release captured cells, and dependence on expensive instrumentation for enrichment or subsequent characterization . In an attempt to take a step forward to overcome the aforementioned limitation, an inverted microchip integrating silicon nanowires (SiNW) and multifunctional magnetic nanocomposites based on anti‐EpCAM‐Fe 3 O 4 @C6/Ce6@silane (anti‐epithelial cell adhesion molecule (EpCAM)) was designed to enhanced capture efficiency and purity of CTCs . The system allows real‐time detection and photodynamic therapy of CTCs and showed improved capture purity of CTCs to 90% and CTCs' capture efficiencies of 90.3% and 82% in culture medium and blood samples, respectively.…”

Section: Representative Biomedical Applicationsmentioning

confidence: 99%

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Advances in Magnetic Nanoparticles for Biomedical Applications

Cardoso

Francesko

Ribeiro

et al. 2017

Adv Healthcare Materials

491

311

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Magnetic nanoparticles (NPs) are emerging as an important class of biomedical functional nanomaterials in areas such as hyperthermia, drug release, tissue engineering, theranostic, and lab-on-a-chip, due to their exclusive chemical and physical properties. Although some works can be found reviewing the main application of magnetic NPs in the area of biomedical engineering, recent and intense progress on magnetic nanoparticle research, from synthesis to surface functionalization strategies, demands for a work that includes, summarizes, and debates current directions and ongoing advancements in this research field. Thus, the present work addresses the structure, synthesis, properties, and the incorporation of magnetic NPs in nanocomposites, highlighting the most relevant effects of the synthesis on the magnetic and structural properties of the magnetic NPs and how these effects limit their utilization in the biomedical area. Furthermore, this review next focuses on the application of magnetic NPs on the biomedical field. Finally, a discussion of the main challenges and an outlook of the future developments in the use of magnetic NPs for advanced biomedical applications are critically provided.

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Section: Representative Biomedical Applicationsmentioning

confidence: 99%

Section: Representative Biomedical Applicationsmentioning

confidence: 99%

Advances in Magnetic Nanoparticles for Biomedical Applications

Cardoso

Francesko

Ribeiro

et al. 2017

Adv Healthcare Materials

491

311

View full text Add to dashboard Cite

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“…Shen and Park described a microfluidic magnetic separation system that can separate subpopulations of macrophage Raw 264.7 cells depending on their absorption capacity [163]. Xu et al developed a microfluidic setup for high purity isolation of CTCs from the whole blood [244]. They demonstrated effective immunomagnetic labeling of MCF-7 human breast cancer, SGC 7901 human gastric cancer, Hela human cervical cancer, and PC3 human prostate cancer cell lines with magnetic composite particles along with isolation from RBCs and leucocytes.…”

Section: Magnetic Cell Separationmentioning

confidence: 99%

Detection of Rare Objects by Flow Cytometry: Imaging, Cell Sorting, and Deep Learning Approaches

Voronin

Kozlova

Verkhovskii

et al. 2020

IJMS

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Flow cytometry nowadays is among the main working instruments in modern biology paving the way for clinics to provide early, quick, and reliable diagnostics of many blood-related diseases. The major problem for clinical applications is the detection of rare pathogenic objects in patient blood. These objects can be circulating tumor cells, very rare during the early stages of cancer development, various microorganisms and parasites in the blood during acute blood infections. All of these rare diagnostic objects can be detected and identified very rapidly to save a patient’s life. This review outlines the main techniques of visualization of rare objects in the blood flow, methods for extraction of such objects from the blood flow for further investigations and new approaches to identify the objects automatically with the modern deep learning methods.

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“…The development of affinity-based techniques results in the improvement of the efficiency of CTCs separation from other components of blood and creation of the opportunity for cells segregation based on the abundance of surface markers expression [117, 130, 131]. This progress is possible among others through magnetic affinity-selection achieved by magnetic ranking cytometry (MagRC) [111, 132] and employment of magnetic items such as beads [17, 131, 133] and nanoparticles [17, 117, 130, 134, 135] coated with antibodies [131, 134], aptamers [17, 130] and peptides [121]. Employment of magnetic items permits the purification achievement by target cells gathering via a magnetic field [17], or by application of a magnetic sorter [131].…”

Section: Circulating Tumor Cellsmentioning

confidence: 99%

“…The enhancement of capture efficacy can also be obtained by Fc-domain EpCAM antibody modification [120]. The capture yield can be increased by antibodies’ immobilization at various nanostructures such as nanopillars [15], nanofibers [136], nanorods [137] or nanowires [135]. They were reported to be composed or covered with gold nanoparticles [138], graphene oxide [122], or TiO 2 [137] as they were accounted to augment the cells’ immobilization on various surfaces and enhance the cells’ capture.…”

Section: Circulating Tumor Cellsmentioning

confidence: 99%

Microfluidics for studying metastatic patterns of lung cancer

et al. 2019

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The incidence of lung cancer continues to rise worldwide. Because the aggressive metastasis of lung cancer cells is the major drawback of successful therapies, the crucial challenge of modern nanomedicine is to develop diagnostic tools to map the molecular mechanisms of metastasis in lung cancer patients. In recent years, microfluidic platforms have been given much attention as tools for novel point-of-care diagnostic, an important aspect being the reconstruction of the body organs and tissues mimicking the in vivo conditions in one simple microdevice. Herein, we present the first comprehensive overview of the microfluidic systems used as innovative tools in the studies of lung cancer metastasis including single cancer cell analysis, endothelial transmigration, distant niches migration and finally neoangiogenesis. The application of the microfluidic systems to study the intercellular crosstalk between lung cancer cells and surrounding tumor microenvironment and the connection with multiple molecular signals coming from the external cellular matrix are discussed. We also focus on recent breakthrough technologies regarding lab-on-chip devices that serve as tools for detecting circulating lung cancer cells. The superiority of microfluidic systems over traditional in vitro cell-based assays with regard to modern nanosafety studies and new cancer drug design and discovery is also addressed. Finally, the current progress and future challenges regarding printable and paper-based microfluidic devices for personalized nanomedicine are summarized. Electronic supplementary material The online version of this article (10.1186/s12951-019-0492-0) contains supplementary material, which is available to authorized users.

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High purity microfluidic sorting and in situ inactivation of circulating tumor cells based on multifunctional magnetic composites

Cited by 33 publications

References 40 publications

Advances in Magnetic Nanoparticles for Biomedical Applications

Advances in Magnetic Nanoparticles for Biomedical Applications

Detection of Rare Objects by Flow Cytometry: Imaging, Cell Sorting, and Deep Learning Approaches

Microfluidics for studying metastatic patterns of lung cancer

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