High-purity magnesium interference screws promote fibrocartilaginous entheses regeneration in the anterior cruciate ligament reconstruction rabbit model via accumulation of BMP-2 and VEGF

Peng, Chong; Han, Pei; Zhao, Changli; Zhang, Shaoxiang; Wu, Hao; Ni, Jiahua; Hou, Peng; Zhang, Yuanzhuang; Liu, Jingyi; Xu, Huibi; Liu, Shen; Zhang, Xiaonong; Zheng, Yufeng; Chai, Yimin

doi:10.1016/j.biomaterials.2015.12.005

Cited by 134 publications

(125 citation statements)

References 42 publications

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“…Bone scaffolds themselves stimulate the osteogenic differentiation of hBMSCs by activating the bone related gene/protein expression . ALP is an early marker for osteoblastic differentiation, RUNX2 is an essential factor for bone and hypertophic cartilage formation, and BMP‐2 is critical in tendon‐bone junction development and fibrocartilage entheses formation . These proteins support the formation of bone‐like nodules, which are the basis for further matrix mineralization .…”

Section: Resultsmentioning

confidence: 99%

“…ALP is an early marker for osteoblastic differentiation, RUNX2 is an essential factor for bone and hypertophic cartilage formation, and BMP‐2 is critical in tendon‐bone junction development and fibrocartilage entheses formation . These proteins support the formation of bone‐like nodules, which are the basis for further matrix mineralization . Figure (A) indicates that Sr 2+ ions can be released from the Ag‐MgSrFe/CS composite scaffold, and arrive at approximately 0.47 mg/L after soaking in SBF for 96 h. The released Sr 2+ ions from the MgSrFe/CS and Ag‐MgSrFe/CS composite scaffolds contribute to high activity of the early osteogenic protein ALP, great secretion of the ECM mineralization, and high expression levels of osteogenic‐related gene RUNX2 and BMP‐2 (Figure ).…”

Section: Resultsmentioning

confidence: 99%

“…51,52 These proteins support the formation of bone-like nodules, which are the basis for further matrix mineralization. 51,52 Figure 4 related gene RUNX2 and BMP-2 ( Figure 6). In contrast, the hBMSCs on the MgFe/CS and Ag-MgFe/CS composite scaffolds without Sr element exhibit low ALP activity, small ECM mineralization and low expression levels of RUNX2 and BMP-2 ( Figure 6).…”

Section: Osteoinductivity Of Ag-loaded Ldh/cs Composite Scaffoldmentioning

confidence: 99%

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Ag‐loaded MgSrFe‐layered double hydroxide/chitosan composite scaffold with enhanced osteogenic and antibacterial property for bone engineering tissue

Cao

Chen

et al. 2017

J Biomed Mater Res

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Bone tissue engineering scaffolds for the reconstruction of large bone defects should simultaneously promote osteogenic differentiation and avoid postoperative infection. Herein, we develop, for the first time, Ag-loaded MgSrFe-layered double hydroxide/chitosan (Ag-MgSrFe/CS) composite scaffold. This scaffold exhibits three-dimensional interconnected macroporous structure with a pore size of 100-300 μm. The layered double hydroxide nanoplates in the Ag-MgSrFe/CS show lateral sizes of 200-400 nm and thicknesses of ∼50 nm, and the Ag nanoparticles with particle sizes of ∼20 nm are uniformly dispersed on the scaffold surfaces. Human bone marrow-derived mesenchymal stem cells (hBMSCs) present good adhesion, spreading, and proliferation on the Ag-MgSrFe/CS composite scaffold, suggesting that the Ag and Sr elements in the composite scaffold have no toxicity to hBMSCs. When compared with MgFe/CS composite scaffold, the Ag-MgSrFe/CS composite scaffold has better osteogenic property. The released Sr ions from the composite scaffold enhance the alkaline phosphatase activity of hBMSCs, promote the extracellular matrix mineralization, and increase the expression levels of osteogenic-related RUNX2 and BMP-2. Moreover, the Ag-MgSrFe/CS composite scaffold possesses good antibacterial property because the Ag nanoparticles in the composite scaffold effectively prevent biofilm formation against S. aureus. Hence, the Ag-MgSrFe/CS composite scaffold with excellent osteoinductivity and antibacterial property has a great potential for bone tissue engineering. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 863-873, 2018.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Osteoinductivity Of Ag-loaded Ldh/cs Composite Scaffoldmentioning

confidence: 99%

See 1 more Smart Citation

Ag‐loaded MgSrFe‐layered double hydroxide/chitosan composite scaffold with enhanced osteogenic and antibacterial property for bone engineering tissue

Cao

Chen

et al. 2017

J Biomed Mater Res

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“…One of the normal functions of VEGF, a signaling protein produced by cells that stimulates vasculogenesis and angiogenesis, is to create new blood vessels after injury. Presumably, an increase in VEGF expression in the Achilles tendon induced by the action of a drug would be very beneficial to endogenous healing of the tendon and thus would improve the recovery effect . The putative source of endogenous progenitor cells for tendon repair is likely activated by heparin, which induces cell migration into the wound area, a function that was identified recently …”

Section: Discussionmentioning

confidence: 99%

Electrospun Heparin‐Loaded Core–Shell Nanofiber Sutures for Achilles Tendon Regeneration In Vivo

Zhou

Jing

et al. 2018

Macromolecular Bioscience

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Achilles tendon reconstruction surgery is the primary clinical method for repairing acute Achilles tendon ruptures. However, the efficacy of the postoperative healing process and the recovery of physiological function are inadequate. This study examines the healing mechanism of ruptured rat Achilles tendons seamed with heparin-loaded core-shell fiber sutures fabricated via near-field electrospinning. High-heparin-concentration sutures (PPH3.0) perform better than the low-heparin-concentration sutures and commercial sutures (CSs). The PPH3.0 suture recruits fewer inflammatory cells and shows good histocompatibility in peritoneal implantation experiments. Staining of the Achilles tendon rupture repair zone demonstrates that a high heparin concentration in sutures reduces immune-inflammatory responses. Immunohistochemical analysis reveals that the transforming growth factor-β staining scores of the PPH3.0 sutures are not significantly different from those of the corresponding control group but are significantly different from those of the CSs and non-heparin-loaded-suture groups. According to vascular endothelial growth factor (VEGF) analysis, the concentration of VEGF in the group treated with the PPH3.0 suture increases by 37.5% compared with that in its control group. No significant difference in tension strength is observed between the PPH3.0 group and healthy Achilles tendons. These findings illustrate that this novel method effectively treats Achilles tendon rupture and promotes healing and regeneration.

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“…Most recently, Cheng et al . reported that the use of Mg fixators may favor the formation of fibrocartilage at the intra-articular aperture in rabbits after ACL reconstruction25. Cheng et al .…”

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confidence: 99%

Biodegradable Magnesium Screws Accelerate Fibrous Tissue Mineralization at the Tendon-Bone Insertion in Anterior Cruciate Ligament Reconstruction Model of Rabbit

Wang

et al. 2017

Sci Rep

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The incorporation of tendon graft into bone tunnel is one of the most challenging clinical issues in anterior cruciate ligament (ACL) reconstruction. As a biodegradable metal, Mg has appropriate mechanical strength and osteoinductive effects, thus may be a promising alternative to commercialized products used for graft fixation. Therefore, it was hypothesized that Mg based interference screws would promote tendon graft-bone junction healing when compared to Ti screws. Herein, we compared the effects of Mg and Ti screws on tendon graft healing in rabbits with ACL reconstruction via histological, HR-pQCT and mechanical analysis. The histological results indicated that Mg screws significantly improved the graft healing quality via promoting mineralization at the tendon graft enthesis. Besides, Mg screws significantly promoted bone formation in the peri-screw region at the early healing stage. Importantly, Mg screws exhibited excellent corrosion resistance and the degradation of Mg screws did not induce bone tunnel widening. In tensile testing, there were no significant differences in the load to failure, stress, stiffness and absorption energy between Mg and Ti groups due to the failure mode at the midsubstance. Our findings demonstrate that Mg screws can promote tendon graft healing after ACL reconstruction, implying a potential alternative to Ti screws for clinical applications.

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High-purity magnesium interference screws promote fibrocartilaginous entheses regeneration in the anterior cruciate ligament reconstruction rabbit model via accumulation of BMP-2 and VEGF

Cited by 134 publications

References 42 publications

Ag‐loaded MgSrFe‐layered double hydroxide/chitosan composite scaffold with enhanced osteogenic and antibacterial property for bone engineering tissue

Ag‐loaded MgSrFe‐layered double hydroxide/chitosan composite scaffold with enhanced osteogenic and antibacterial property for bone engineering tissue

Electrospun Heparin‐Loaded Core–Shell Nanofiber Sutures for Achilles Tendon Regeneration In Vivo

Biodegradable Magnesium Screws Accelerate Fibrous Tissue Mineralization at the Tendon-Bone Insertion in Anterior Cruciate Ligament Reconstruction Model of Rabbit

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