High productivity of human recombinant beta-interferon from a low-temperature perfusion culture

Rodrı́guez, José F.; Spearman, Maureen; Tharmalingam, Tharmala; Sunley, Kevin; Lodewyks, Carly; Huzel, N.; Butler, Michael

doi:10.1016/j.jbiotec.2010.09.959

Cited by 53 publications

(27 citation statements)

References 68 publications

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“…Overall, the quality attributes from perfusion cultures are acceptable, and the observed variations are within the literature reported levels for perfusion cultures (Goldman et al, 1998;Meuwly et al, 2006;Rodriguez et al, 2010). For future studies, better control of the foam level and reduction of the antifoam impact on O 2 sparge rate/culture pH will be pursued, such as supplementing antifoam in perfusion media to have a more evenly distributed antifoam addition (Tashiro et al, 2005).…”

Section: Product Quality In Perfusion Cultures Without Ph Controlsupporting

confidence: 63%

High-density mammalian cell cultures in stirred-tank bioreactor without external pH control

Chen

2016

Journal of Biotechnology

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Section: Product Quality In Perfusion Cultures Without Ph Controlsupporting

confidence: 63%

High-density mammalian cell cultures in stirred-tank bioreactor without external pH control

Chen

2016

Journal of Biotechnology

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“…The constant environment of the perfusion contributes to the stability and improvement of the quality attributes with time, e.g. glycosylation (Goldman et al 1998), sialilation (Goh et al 2014), aggregation (Rodriguez et al 2010).…”

Section: Protein Qualitymentioning

confidence: 99%

Perfusion Processes

Chotteau

2014

Cell Engineering

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The interest for perfusion is increasing nowadays. This new focus has emerged from a synergy of a demand for disposable equipment and the availability of robust cell separation device, as well as the need for higher flexibility and lower investment cost. The cell separation devices mostly used today are based on filtration, i.e. alternating flow filtration, tangential flow filtration, spin-filter, or acceleration/gravity, i.e. inclined settler, centrifuge, acoustic settler. This paper gives an introduction to the basic concepts of perfusion and its practical implementation. It reviews the actual cell separation devices and describes the approaches used in the field to develop and optimize the perfusion processes.

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“…Reduction of culture temperature below 37 C increases productivity in many production systems (Fan et al 2010;Rodriguez et al 2010;Sunley et al 2008;Trummer et al 2006). However, lower culture temperatures can have variable effects on glycosylation.…”

Section: Culture Parameters Affecting Glycosylationmentioning

confidence: 99%

Glycosylation in Cell Culture

Spearman

Butler

2014

Cell Engineering

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Glycosylation affects many general functional factors of a glycoprotein, such as stability, inhibition of proteolysis, solubility, aggregation, as well as other attributes critical to its use as a biotherapeutic. Therefore our understanding of the glycosylation pathway, factors that affect it, and our ability to manipulate glycosylation are essential in producing superior biotherapeutics. The complex synthetic pathway of N-linked glycosylation occurs in both the endoplasmic reticulum and Golgi and involves a large number of precursors and enzymes, leading to a large array of possible glycan structures. Thus, variability in glycosylation may be influenced by numerous factors that affect this pathway, such as host cell type, nutrient levels and supplements, dissolved oxygen, pH, temperature, and by-product accumulation, and thus must be closely monitored. Better analytical techniques have allowed linking specific glycan structures to functionality of glycoproteins, which have led to efforts to modify glycosylation through genetic engineering, sequence-interfering RNA (siRNA), glycosylation inhibitors or chemoenzymatic modification.

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High productivity of human recombinant beta-interferon from a low-temperature perfusion culture

Cited by 53 publications

References 68 publications

High-density mammalian cell cultures in stirred-tank bioreactor without external pH control

High-density mammalian cell cultures in stirred-tank bioreactor without external pH control

Perfusion Processes

Glycosylation in Cell Culture

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