High production of acetone–butanol–ethanol with high cell density culture by cell-recycling and bleeding

Tashiro, Yukihiro; Takeda, Kazuya; Kobayashi, Genta; Sonomoto, Kenji

doi:10.1016/j.jbiotec.2005.05.031

Cited by 122 publications

(62 citation statements)

References 31 publications

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“…For example, cell recycling has been shown to improve cell concentration and productivity in the ABE process. [251]. Cell recycling, usually achieved with a semi-permeable membrane, is an interesting approach as it allows high cell concentrations and clean permeates for further separation [250].…”

Section: Fermentation and Bioreactor Designmentioning

confidence: 99%

Commercial Biomass Syngas Fermentation

2012

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Abstract:The use of gas fermentation for the production of low carbon biofuels such as ethanol or butanol from lignocellulosic biomass is an area currently undergoing intensive research and development, with the first commercial units expected to commence operation in the near future. In this process, biomass is first converted into carbon monoxide (CO) and hydrogen (H 2 )-rich synthesis gas (syngas) via gasification, and subsequently fermented to hydrocarbons by acetogenic bacteria. Several studies have been performed over the last few years to optimise both biomass gasification and syngas fermentation with significant progress being reported in both areas. While challenges associated with the scale-up and operation of this novel process remain, this strategy offers numerous advantages compared with established fermentation and purely thermochemical approaches to biofuel production in terms of feedstock flexibility and production cost. In recent times, metabolic engineering and synthetic biology techniques have been applied to gas fermenting organisms, paving the way for gases to be used as the feedstock for the commercial production of increasingly energy dense fuels and more valuable chemicals.

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Section: Fermentation and Bioreactor Designmentioning

confidence: 99%

Commercial Biomass Syngas Fermentation

2012

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“…76,77 This approach helped to maintain high productivities for long period without cell degeneration. 76 A continuous fermentation of C. pasteurianum MBEL-GLY2 (hyper-producing strain) with cell recycling was carried out to get the ABE and the butanol productivity of 8.3 and 7.8 g.l -1 .h -1 respectively, at the dilution rate of 0.9 h -1 . 77 Zheng et al 78 have reported the continuous butanol production system from glucose and xylose with high cell-density generated by cell recycling.…”

Section: Single Stage Reactors With Cell Recyclingmentioning

confidence: 99%

Biobutanol: the outlook of an academic and industrialist

et al. 2013

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The gradual shift of transportation fuels from oil based fuels to the alternative fuel resources and worldwide demand for energy has been the impetus for research to produce alcohol biofuels from renewable resources. Current bioethanol and biodiesel can, however, not cover an increasing demand for biofuels. Hence, there is an extensive need for advanced biofuels with superior fuel properties. The present review is focused on the developments of biobutanol, which is regarded to be superior to bioethanol in terms of energy density and hygroscopicity. Although acetone-butanolethanol (ABE) fermentation is one of the oldest large-scale fermentation processes, butanol yield by anaerobic fermentation remains sub-optimal. For sustainable industrial scale butanol production, a number of obstacles need to be addressed including choice of feedstock, low product yield, product toxicity to production strain, multiple end-products and downstream processing of alcohol mixtures.Metabolic engineering provides a means for fermentation improvements. Different strategies are employed in the metabolic engineering of Clostridia that aim to enhance the solvent production, improve selectivity for butanol production, and increase the tolerance of Clostridia to solvents. The introduction and expression of a non-clostridial butanol producing pathway in E. coli is most promising strategy for butanol biosynthesis. Several rigorous kinetic and physiological models for fermentation have been formulated, which form useful tool for optimization of the process. Due to the lower butanol titers in the fermentation broth, simultaneous fermentation and product removal techniques have been developed to improve production economics. With the use of new strains, inexpensive substrates, and superior reactor designs, economic ABE fermentation may further attract an attention of researchers all over the world. The present review is attempting to provide an overall outlook on discoveries and strategies that are being developed for commercial n-butanol production.

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“…Its efficiency was previously demonstrated for the production of several compounds like lactic acid (Kwon et al, 2001), ethanol (Lee and Chang, 1987), acetonebutanol-ethanol (Ferras et al, 1986;Tashiro et al, 2005). This system is particularly well suited for the anaerobic fermentation in which the rate limiting step is the low biomass concentration.…”

Section: Discussionmentioning

confidence: 99%

A new process for the continuous production of succinic acid from glucose at high yield, titer, and productivity

Meynial-Salles

Dorotyn

Soucaille

2007

Biotech & Bioengineering

156

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A novel three stages continuous fermentation process for the bioproduction of succinic acid at high concentration, productivity and yield using A. succiniciproducens was developed. This process combined an integrated membrane-bioreactor-electrodialysis system. An energetic characterization of A. succiniciproducens during anaerobic cultured in a cell recycle bioreactor was done first. The very low value of Y(ATP) obtained suggests that an ATP dependent mechanism of succinate export is present in A. succiniciproducens. Under the best culture conditions, biomass concentration and succinate volumetric productivity reach values of 42 g/L and 14.8 g/L.h. These values are respectively 28 and 20 times higher compared to batch cultures done in our laboratory. To limit end-products inhibition on growth, a mono-polar electrodialysis pilot was secondly coupled to the cell recycle bioreactor. This system allowed to continuously remove succinate and acetate from the permeate and recycle an organic acids depleted solution in the reactor. The integrated membrane-bioreactor-electrodialysis process produced a five times concentrated succinate solution (83 g/L) compared to the cell recycle reactor system, at a high average succinate yield of 1.35 mol/mol and a slightly lower volumetric productivity of 10.4 g/L.h. The process combined maximal production yield to high productivity and titer and could be economically viable for the development of a biological route for succinic acid production.

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High production of acetone–butanol–ethanol with high cell density culture by cell-recycling and bleeding

Cited by 122 publications

References 31 publications

Commercial Biomass Syngas Fermentation

Commercial Biomass Syngas Fermentation

Biobutanol: the outlook of an academic and industrialist

A new process for the continuous production of succinic acid from glucose at high yield, titer, and productivity

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