High production of 1,3-propanediol from glycerol by Clostridium butyricum VPI 3266 in a simply controlled fed-batch system

Saint-Amans, Sylvie; Perlot, P.; Goma, G.; Soucaille, Philippe

doi:10.1007/bf00133962

Cited by 83 publications

(44 citation statements)

References 9 publications

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“…The colouring is based on the differences of composition of the bacteria wall. Indeed, if bacteria are positive Gram, they seem purple and rose if they are negative Gram [13].…”

Section: Microbiology Methodsmentioning

confidence: 99%

1,3-Propanediol Production by Clostridium Butyricum in Various Fed- Batch Feeding Strategies

Ahmed¹,

Naouel²

2012

J Biotechnol Biomaterial

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The aim of this study is to propose different fed-batch strategies during the fermentation of glycerol into 1,3-Propanediol, which show the influence of the initial concentration in glycerol and the feed flow on the behaviour of Clostridium butyricum. The kinetic study of the conversion of glycerol into 1,3-Propanediol by Clostridium butyricum, showed that according to the initial concentration of glycerol and feed flow, this strain displays two different metabolic behaviours. This strain directs its metabolism towards its traditional metabolic pathways (production of 1,3-Propanediol, butyric and acetic acid), when the culture was started with a high initial concentration of glycerol and was fed with a weak rate. The high initial concentration of glycerol causes a deceleration of growth rate, which led to an increase in the growth phase duration. Feeding by a weak flow allowed maintaining the cells in full growth phase by providing the necessary substrate for its growth and its maintenance and, at the same time, to avoid the glycerol accumulation in the medium. A high feed flow involved a glycerol accumulation in the culture medium, which obliged the bacterium to direct its metabolism towards the production of acetic acid in significant quantities. The growth phase always showed the same duration, yielding similar final concentrations in biomass and metabolites, an identical metabolic behaviour was observed when the culture begin with a low initial concentration of glycerol, and whatever the flow of substrate applied. This behaviour may be explained by a growth inhibition caused by the accumulation of 1,3-Propanediol in concentrations of 20-25 g/L.

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“…The colouring is based on the differences of composition of the bacteria wall. Indeed, if bacteria are positive Gram, they seem purple and rose if they are negative Gram [13].…”

Section: Microbiology Methodsmentioning

confidence: 99%

1,3-Propanediol Production by Clostridium Butyricum in Various Fed- Batch Feeding Strategies

Ahmed¹,

Naouel²

2012

J Biotechnol Biomaterial

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“…with glycerol as the sole carbon source has resulted in 1,3-PDO titers greater than 60 g/l from both isolated and engineered strains of K. pneumoniae [31][32][33], C. butyricum [31,[34][35][36][37], and recombinant Clostridium acetobutylicum [34,38]. Due in part to the large number of studies and interest in the conversion of glycerol into 1,3-PDO, this topic has also been extensively reviewed [20,39].…”

Section: Trends In Biotechnologymentioning

confidence: 99%

Anaerobic fermentation of glycerol: a platform for renewable fuels and chemicals

Clomburg

González

2013

Trends in Biotechnology

263

184

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“…Although C. butyricum VPI 3266 is probably the best producer of 1,3-propanediol (42), no physiological investigation has been carried out regarding the conversion of glycerol to this diol. In this report, we studied the metabolic flexibility of C. butyricum in response to an increase in NAD(P)H pressure resulting from the use of glycerol.…”

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confidence: 99%

Regulation of Carbon and Electron Flow in Clostridium butyricum VPI 3266 Grown on Glucose-Glycerol Mixtures

et al. 2001

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The metabolism of Clostridium butyricum was manipulated at pH 6.5 and in phosphate-limited chemostat culture by changing the overall degree of reduction of the substrate using mixtures of glucose and glycerol. Cultures grown on glucose alone produced only acids (acetate, butyrate, and lactate) and a high level of hydrogen. In contrast, when glycerol was metabolized, 1,3-propanediol became the major product, the specific rate of acid formation decreased, and a low level of hydrogen was observed. Glycerol consumption was associated with the induction of (i) a glycerol dehydrogenase and a dihydroxyacetone kinase feeding glycerol into the central metabolism and (ii) an oxygen-sensitive glycerol dehydratase and an NAD-dependent 1,3-propanediol dehydrogenase involved in propanediol formation. The redirection of the electron flow from hydrogen to NADH formation was associated with a sharp decrease in the in vitro hydrogenase activity and the acetyl coenzyme A (CoA)/free CoA ratio that allows the NADH-ferredoxin oxidoreductase bidirectional enzyme to operate so as to reduce NAD in this culture. The decrease in acetate and butyrate formation was not explained by changes in the concentration of phosphotransacylases and acetate and butyrate kinases but by changes in in vivo substrate concentrations, as reflected by the sharp decrease in the acetyl-CoA/free CoA and butyryl-CoA/free CoA ratios and the sharp increase in the ATP/ADP ratio in the culture grown with glucose and glycerol compared with that in the culture grown with glucose alone. As previously reported for Clostridium acetobutylicum (L. Girbal, I. Vasconcelos, and P. Soucaille, J. Bacteriol. 176:6146-6147, 1994), the transmembrane pH of C. butyricum is inverted (more acidic inside) when the in vivo activity of hydrogenase is decreased (cultures grown on glucose-glycerol mixture). For both cultures, the stoichiometry of the H ؉ ATPase was shown to remain constant and equal to 3 protons exported per molecule of ATP consumed.By-products of the food industry have great potential for the production of intermediates for the chemical industry; however, until now only a few applications have been found. Glycerol, a by-product with three atoms of carbon, can easily enter the metabolic pathways of several microorganisms to produce a wide range of compounds.Bioconversion of glycerol to 1,3-propanediol is already known for several bacterial strains, e.g., Lactobacillus brevis and Lactobacillus buchnerii (44,46), Bacillus welchii (23), Citrobacter freundii and Klebsiella pneumoniae (32,40,47), Clostridium acetobutylicum (14), Clostridium pasteurianum (35), and Clostridium butyricum (3, 21, 43). Anaerobic metabolic pathways of glycerol catabolism have, until now, only been completely characterized in the genera Klebsiella (11,12,13,36) and Citrobacter (7,8,9,45). According to the experiments done in these microorganisms, glycerol is metabolized in two simultaneous pathways. In the first, an NAD ϩ -dependent glycerol dehydrogenase catalyzes the oxidation of glycerol to dihydrox...

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High production of 1,3-propanediol from glycerol by Clostridium butyricum VPI 3266 in a simply controlled fed-batch system

Cited by 83 publications

References 9 publications

1,3-Propanediol Production by Clostridium Butyricum in Various Fed- Batch Feeding Strategies

1,3-Propanediol Production by Clostridium Butyricum in Various Fed- Batch Feeding Strategies

Anaerobic fermentation of glycerol: a platform for renewable fuels and chemicals

Regulation of Carbon and Electron Flow in Clostridium butyricum VPI 3266 Grown on Glucose-Glycerol Mixtures

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