High prevalence of TT virus infection in Brazilian blood donors

Niel, Christian; Oliveira, Jaqueline Mendes de; Ross, Rudolf Stefan; Gomes, Saint Clair; Roggendorf, Michael; Viazov, Sergei

doi:10.1002/(sici)1096-9071(199903)57:3<259::aid-jmv8>3.0.co;2-2

Cited by 95 publications

(44 citation statements)

References 10 publications

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“…The rate is similar to that in healthy blood donors with the same primers [17,29]. However, a recent study shows a high prevalence of TTV-infected individuals among the healthy donors with other primers [15,28]. It is possible that we could not detect all TTV strains with our system.…”

Section: Discussionsupporting

confidence: 77%

The prevalence of TT virus (TTV) infection and its relationship to hepatitis in children

Iriyama

Kimura

Nishikawa

et al. 1999

Medical Microbiology and Immunology

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TT virus (TTV) is a newly discovered virus from a patient with post-transfusion hepatitis. We investigated the frequency and pathogenesis of TTV infection in children. A semi-nested PCR assay was used to amplify TTV-DNA in serum samples from 254 ambulatory children without liver disease, 20 with hepatitis of unknown etiology, and 18 transfusion recipients or hemophiliacs. In positive samples, TTV-DNA was quantified by real-time quantitative PCR using a fluorescent probe. We detected TTV-DNA in 20% of children with hepatitis of unknown etiology, which was not statistically different from the 23% prevalence in ambulatory children. In transfusion recipients or hemophiliacs, the frequency was higher (50%) than that in ambulatory children (P = 0.01). Among ambulatory children, TTV-DNA was frequently detected in children with acute gastroenteritis (36%). TTV-DNA was detected in 10% of the infants under 6 months old, and 20% of the children from 7 to 12 months old. The prevalence was constant after the age of 1 year; however, the copy number of TTV-DNA was significantly higher in children under 1 year of age (mean: 10(5.4) versus 10(3.8) copies/ml, P= 0.008). Finally, TTV-DNA was quantified serially in three children with chronic hepatitis who were positive for TTV-DNA. The presence or amount of TTV-DNA was unrelated to the serum alanine aminotransferase level. These results indicate that TTV infection is common in children. The larger quantity of TTV-DNA in infants and the high prevalence of TTV in children of all ages suggest that TTV may be transmitted in early childhood. Its relationship to hepatitis is doubtful in children.

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Section: Discussionsupporting

confidence: 77%

The prevalence of TT virus (TTV) infection and its relationship to hepatitis in children

Iriyama

Kimura

Nishikawa

et al. 1999

Medical Microbiology and Immunology

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“…Depending on the identification method used, TTV prevalence may vary from 46.0–62.0% in Brazil [ 21 , 22 ] to 94.0% in Russia [ 23 ] . For TTMV, prevalence ranges between 48.0% in Norway [ 24 ] and 67.0–72.0% in Brazil [ 21 , 22 ] , and TTMDV occurs in at least 40.0% of the general population [ 25 ] . Our results are in accordance with prevalence values reported for TTVs in other populations, except for TTMDV, which has a lower prevalence in subjects selected for this study (31.0%).…”

Section: Discussionmentioning

confidence: 99%

Rapid Detection of Human Torque Teno Viruses Using High-Resolution Melting Analysis

Spandole¹,

Cimponeriu²,

Toma³

et al. 2013

Balkan Journal of Medical Genetics

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Torque teno viruses (TTVs) are recently discovered DNA viruses, with heterogeneous genomes, highly prevalent in populations worldwide. The species that infect humans are Torque teno virus (TTV), Torque teno midi virus (TTMDV) and Torque teno mini virus (TTMV). High-resolution melting analysis (HRMA) is a sensitive and effective method for genotyping and mutation scanning. Up to now, HRMA has not been utilized for detection of TTVs. The aim of this study was to asses if HRMA is suitable for detecting TTVs variants. DNA was extracted from the blood and saliva of 13 healthy subjects for method optimization. Additionally, saliva samples from 100 healthy individuals were collected for estimating the TTVs’ prevalence. Viral DNA was amplified by heminested polymerase chain reaction (PCR). Second round amplicons were used for the HRMA. The samples were analyzed using two fluorescent dyes, SYBR ® Green I and EvaGreen®. The prevalence values for TTV, TTMDV and TTMV were 71.0, 31.0 and 54.0%, respectively. The three major melting curve patterns corresponding to TTV, TTMDV and TTMV on HRMA can be easily distinguished regardless of kit used. Our results showed that HRMA is a rapid and efficient method of detecting human TTVs.

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“…In recent studies, the majority of TTV-positive patients had no biochemical or histological evidence of significant liver damage. TTV-DNA was found to be positive in a high proportion of healthy individuals from various countries (53-93%) [7,10,11]. This suggests the possibility of asymptomatic carriage of TTV and transmission via nonparenteral routes.…”

Section: Introductionmentioning

confidence: 94%

TT Virus Infection and Genotype Distribution in Blood Donors and a Group of Patients from Turkey

Erensoy¹,

Sayıner

Türkoğlu

et al. 2002

Infection

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High prevalence of TT virus infection in Brazilian blood donors

Cited by 95 publications

References 10 publications

The prevalence of TT virus (TTV) infection and its relationship to hepatitis in children

The prevalence of TT virus (TTV) infection and its relationship to hepatitis in children

Rapid Detection of Human Torque Teno Viruses Using High-Resolution Melting Analysis

TT Virus Infection and Genotype Distribution in Blood Donors and a Group of Patients from Turkey

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