Introduction: transfusion-associated infections mainly caused by bacteria or virus among blood donors are of concern to public health. Blood transfusion practices around the world emphasize safety and the protection of human life.Objectives: the aim of this study, is to identify positive serology for syphilis in blood donors.Methodology: A retrospective descriptive study of prevalence was made. We analyzed the database of records of blood donors, screened from 2011 to 2016, with positive syphilis serologies. The demographic data was recorded and analyzed using SPSS software, version 22.0.Results: out of a total of 2734 donors, 436 (20%) had positive syphilis serology. The majority (88%) 384 were men and 52 (12%) women. Ages ranged between 18 and 64 years, with an average of 31.95 ± 8.9.Conclusion: in this study, a seroprevalence rate of 20% was observed for positive syphilis serology, which means the impact of the disease on donors is high, this suggests that screening should be reinforced.