High prevalence of spa types associated with the clonal lineage CC398 among tetracycline-resistant methicillin-resistant Staphylococcus aureus strains in a Spanish hospital

Lozano, Carmen; Rezusta, Antonio; Gómez, Paula; Gómez-Sanz, Elena; Baez, Natalia Soledad; Martín-Saco, Gloria; Zarazaga, Myriam; Torres, Carmén

doi:10.1093/jac/dkr497

Cited by 70 publications

(63 citation statements)

References 11 publications

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“…28 Resistance is mainly Q3 related to tet(M) gene, but not excluded other genes as tet(K) or tet(L), or even a combination of both, as we have found in one of our isolates. 7 An unusual resistance profile (clindamycin resistance but erythromycin susceptibility) has been described in ST398 isolates associated with the presence of vga(A) and vga(C) or lnu(A) genes. 29,30 This unusual profile was found in our study encoded by lnu(A) gene.…”

Section: Discussionmentioning

confidence: 99%

“…[4][5][6] To date, livestock-associated MRSA (LA-MRSA) clones has not been frequently identified in hospitals or nursing homes in Europe, although the spread seems to be dependent on the region and the intensity of pig farming. 7 In Spain in early twenties, the dominant clone was Iberian clone (ST247/SCCmecI) but over the years this clone has reduced its presence. 8 Currently, the most widespread HA-MRSA clone is ST125/SCCmecIV/t067 which is an allelic variant of ST5 pediatric clone.…”

Section: Q2mentioning

confidence: 99%

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Antimicrobial resistance, virulence factors and genetic lineages of hospital-onset methicillin-resistant Staphylococcus aureus isolates detected in a hospital in Zaragoza

González-Domínguez

Seral

Potel

et al. 2015

Enfermedades Infecciosas y Microbiología Clínica

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Please cite this article in press as: González-Domínguez M, et al. b s t r a c tIntroduction: MRSA population dynamics is undergoing significant changes, and for this reason it is important to know which clones are circulating in our nosocomial environment. Materials and methods: A total of 118 MRSA isolates were collected from clinical samples from patients with previous hospital or healthcare contact (named as hospital-onset MRSA (HO-MRSA)) during a one year period. Susceptibility testing was performed by disk diffusion and microdilution. The presence of resistance genes and virulence factors were tested by PCR. All isolates were typed by SCCmec, spa and agr typing. PFGE and MLST were applied to a selection of them.Results: Eighty-three HO-MRSA isolates (70.3%) were resistant to any antibiotic included in the macrolide-lincosamide-streptogramin B group. Among these isolates, the M phenotype was the most frequent (73.5%). One hundred and seven of HO-MRSA isolates (90.7%) showed aminoglycoside resistance. The combination aac(6 )-Ie-aph(2 )-Ia + ant (4 ) El CC5 fue el más prevalente (88,1%) seguido de CC8 (5,9%), CC22 (2,5%), CC398 (2,5%) y CC1 (0,8%). Conclusión: La línea clonal CC5/ST125/t067 fue la más habitual. Esta línea se relacionó con resistencia a aminoglucósidos, y, en menor medida, con macrólidos. La presencia de clones internacionales como EMRSA-15 (CC22/ST22), clones europeos como CC5/ST228, clones comunitarios relacionados con CC1 o CC8 y clones asociados al ganado, como el CC398, se observaron en un bajo porcentaje. ARTICLE IN PRESSG Model EIMC 1282 1-7 2 M. González-Domínguez et al. / Enferm Infecc Microbiol Clin. 2015;xxx(xx):xxx-xxx

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Section: Discussionmentioning

confidence: 99%

Section: Q2mentioning

confidence: 99%

Antimicrobial resistance, virulence factors and genetic lineages of hospital-onset methicillin-resistant Staphylococcus aureus isolates detected in a hospital in Zaragoza

González-Domínguez

Seral

Potel

et al. 2015

Enfermedades Infecciosas y Microbiología Clínica

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“…Since then, much evolution has occurred, and more sequence types are included now [15,16]. Although CC398 is still the most common LA-MRSA worldwide, its prevalence differs geographically; in certain regions, other sequence types are involved, such as ST9 in Asia.…”

Section: General Characteristics Of La-mrsamentioning

confidence: 99%

Livestock-Associated MRSA and Its Current Evolution

Butaye

Argudín

Smith

2016

Curr Clin Micro Rpt

116

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Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) is a relatively recent phenomenon in veterinary medicine. Although in the beginning it was restricted to a single clonal complex (CC), CC398, it has expanded into several clonal complexes, and the diversity of subtypes in the clonal complexes is increasing also. The prevalence of each type is determined somewhat geographically; for instance, the most prevalent clonal complex in Europe is CC398, whereas in Asia, it is CC9. Although few data exist regarding North America, the situation appears to be mixed there. The SCCmec cassettes detected in LA-MRSA are limited mainly to SCCmec IVa and SCCmec V, although non-typeable cassettes and SCCmec type XI, containing mecC, also have been found.The source of the SCCmec in LA-MRSA was discovered to be animals. In searching from which bacteria the SCCmec cassettes in LA-MRSA have been transferred, the most obvious species to consider are the methicillin-resistant non-S. aureus staphylococci (MRNaS). However, very few data are available from those species in animals, and the data that do exist are not detailed enough to determine the origin. Nevertheless, similar cassettes were found in MRNaS, indicating a possible origin that needs to be investigated further.

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“…ST398 MRSA strains may carry and transfer to other S. aureus backgrounds transposons encoding genes for tetracycline resistance (33,34) or have resistance to pleuromutilin and lincosamide antimicrobials (4,(35)(36)(37). ST398 isolated from humans has been found to carry phages that encode human innate immune modulators (38).…”

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Asymptomatic Carriage of Sequence Type 398, spa Type t571 Methicillin-Susceptible Staphylococcus aureus in an Urban Jail: a Newly Emerging, Transmissible Pathogenic Strain

et al. 2013

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Sequence type 398 (ST398) Staphylococcus aureus, frequently carried by livestock, has caused severe human infections and often carries transmissible antibiotic resistance genes. Among methicillin-susceptible S. aureus isolates colonizing Dallas County Jail detainees, 13.2% were ST398, spa type t571, and were genetically similar to human colonization isolates from New York, Chicago, and the Dominican Republic. Sequence type 398 (ST398) methicillin-resistant Staphylococcus aureus (MRSA) is often transmitted among livestock and animal handlers but is uncommonly associated with human disease in the United States (1). We unexpectedly identified ST398/t571 methicillin-sensitive S. aureus (MSSA) as the most common genetic background among MSSA isolates colonizing detainees in the Dallas County Jail. In this study, we compared the pulsed-field gel electrophoresis (PFGE) pulsotypes of isolates from the Dallas jail and from a single patient in Chicago with those of strains isolated in previous studies.Recognized first in Europe (2-9), ST398 MRSA has been isolated in the United States (10), Canada (11), and Latin America (12). ST398 methicillin-susceptible S. aureus (MSSA) isolates have been recovered from pigs (13), humans (14-17), and retail meat (18). Asymptomatic human colonization and infection with ST398 MSSA are rare in the United In January 2009, we screened the anterior nares and the hands of 928 detainees, housed in 68 divisions, called tanks, in the Dallas County Jail, for S. aureus carriage. Tanks had a capacity of 24 to 36 detainees. Detainees can interact with others within a single tank but have little interaction with those in other tanks. The study was approved by the Institutional Review Boards at the University of Chicago Medical Center and the University of Texas-Southwestern Medical Center. Recovered MSSA isolates were genotyped from subjects housed in a stratified random sample of 26 of the 68 tanks; all MRSA isolates were genotyped.In a separate study of S. aureus colonization among patients and household contacts (24), we also identified one patient in Chicago with ST398/t571 MSSA colonization.Multilocus sequence typing (MLST) (25), spa typing (26), PCR for the Panton-Valentine leukocidin (PVL) genes (27), and PFGE using Cfr9I digestion (22, 28) were performed. Antimicrobial susceptibilities were determined by automated testing (29).Among the 345 subjects in the 26 selected tanks, 110 (31.9%) carried MSSA. Of 158 MSSA isolates identified, 152 were available, and 34 MLST types were identified. Twenty isolates (13%) were ST398, the most common ST; all lacked PVL genes. Of the 345 subjects, the 16 (4.6%) who carried the 20 ST398 MSSA isolates were more likely than carriers of other MSSA genetic backgrounds to be female (P ϭ 0.0009) and older (P ϭ 0.01) ( Table 1).Among the 20 ST398 isolates, 18 were susceptible to ciprofloxacin, gentamicin, rifampin, and trimethoprim-sulfamethoxazole and resistant to clindamycin and erythromycin. The two other isolates differed in that one was susceptible to clindamyc...

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High prevalence of spa types associated with the clonal lineage CC398 among tetracycline-resistant methicillin-resistant Staphylococcus aureus strains in a Spanish hospital

Abstract: Tetracycline resistance could be a good marker for MRSA CC398, although this resistance can also be found in other lineages.

Cited by 70 publications

References 11 publications

Antimicrobial resistance, virulence factors and genetic lineages of hospital-onset methicillin-resistant Staphylococcus aureus isolates detected in a hospital in Zaragoza

Antimicrobial resistance, virulence factors and genetic lineages of hospital-onset methicillin-resistant Staphylococcus aureus isolates detected in a hospital in Zaragoza

Livestock-Associated MRSA and Its Current Evolution

Asymptomatic Carriage of Sequence Type 398, spa Type t571 Methicillin-Susceptible Staphylococcus aureus in an Urban Jail: a Newly Emerging, Transmissible Pathogenic Strain

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