High prevalence of Pseudomonas aeruginosa carriage in residents of French and German long-term care facilities

Martak, Daniel; Gbaguidi‐Haore, Houssein; Meunier, Alexandre; Valot, Benoı̂t; Conzelmann, Nadine; Eib, Michael; Autenrieth, Ingo B.; Slekovec, C.; Tacconelli, Evelina; Bertrand, Xavier; Peter, Silke; Hocquet, Didier; Guther, Julia

doi:10.1016/j.cmi.2022.05.004

Cited by 14 publications

(3 citation statements)

References 27 publications

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“…The clone here described exhibits concerning characteristics, including antibiotic resistance genes associated with transferable elements, several virulence factors, a resistance to new antibiotics, and the ability to form biofilms. This outbreak persisted for at least one year in the ICU, suggesting the presence of an environmental source as documented in previous studies, where biofilms could play an essential role [27,32,33]. Additionally, an enhanced ability to develop biofilms has been proposed as an underlying factor behind the success of high-risk clones [7].…”

Section: Discussionsupporting

confidence: 62%

“…As suggested by data obtained from the WGS of five representative strains, the outbreak described here was caused by P. aeruginosa belonging to sequence type ST309 and serotype O11. ST309 has recently been proposed as a high-risk clone given its wide distribution among different continents including Asia, Europe, Oceania, and the Americas [8,32]. This lineage was initially identified in low proportions as a minor clone in Greece and Korea, with the latter being associated with bla VIM-2 ; subsequent reports have documented clonal spread.…”

Section: Discussionmentioning

confidence: 99%

“…This lineage was initially identified in low proportions as a minor clone in Greece and Korea, with the latter being associated with bla VIM-2 ; subsequent reports have documented clonal spread. These include a massive and persistent colonization of a dental care unit waterline in a University Hospital in France [33], isolates from children with bacteremia in Mexico [10], extensively drug-resistant clinical isolates from the United States [9], the Philippines [34], and Brazil [8], and intestinal colonization and environmental samples from a long-term care facility in France [32]. ST309 clones are reported to carry several acquired resistance genes in class one integrons, including ESBLs (bla GES-19, -20, -26 ), carbapenemases (bla VIM-2 and bla IMP-15 ), aminoglycoside-modifying enzymes (aadA1, aacA4, aac(6 ′ )-Il and aac(6 ′ )-33), and fluoroquinolone resistance genes (qnrVC1), among others [8].…”

Section: Discussionmentioning

confidence: 99%

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Outbreak of Pseudomonas aeruginosa High-Risk Clone ST309 Serotype O11 Featuring blaPER-1 and qnrVC6

Papa-Ezdra,

Outeda,

Cordeiro

et al. 2024

Antibiotics

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Pseudomonas aeruginosa is a leading cause of hospital-acquired infections worldwide. Biofilm production, antibiotic resistance, and a wide range of virulence factors contribute to their persistence in nosocomial environments. We describe an outbreak caused by a multidrug-resistant P. aeruginosa strain in an ICU. Antibiotic susceptibility was determined and blaPER-1 and qnrVC were amplified via PCR. Clonality was determined using PFGE and biofilm formation was studied with a static model. A combination of antibiotics was assessed on both planktonic cells and biofilms. WGS was performed on five isolates. All isolates were clonally related, resistant to ceftazidime, cefepime, amikacin, and ceftolozane-tazobactam, and harbored blaPER-1; 11/19 possessed qnrVC. Meropenem and ciprofloxacin reduced the biofilm biomass; however, the response to antibiotic combinations with rifampicin was different between planktonic cells and biofilms. WGS revealed that the isolates belonged to ST309 and serotype O11. blaPER-1 and qnrVC6 were associated with a tandem of ISCR1 as part of a complex class one integron, with aac(6′)-Il and ltrA as gene cassettes. The structure was associated upstream and downstream with Tn4662 and flanked by direct repeats, suggesting its horizontal mobilization capability as a composite transposon. ST309 is considered an emerging high-risk clone that should be monitored in the Americas.

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