High prevalence of mcr-1 encoding colistin resistance and first identification of blaCTX-M-55 in ESBL/CMY-2-producing Escherichia coli isolated from chicken faeces and retail meat in Tunisia

Hassen, Bilel; Abbassi, Mohamed Salah; Ruiz-Ripa, Laura; Mama, Olouwafemi Mistourath; Hassen, Abdennaceur; Torres, Carmén; Hammami, Salah

doi:10.1016/j.ijfoodmicro.2019.108478

Cited by 64 publications

(63 citation statements)

References 40 publications

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“…Similarly, other studies have shown that the detection rate of E. coli possessing mcr-1 from chicken meat samples collected from Germany [19], China [2], Japan [22], Brazil [23], and Nepal [24] ranged from 1.4-19.5%. More recently, a study in Tunisia showed that the rate of E. coli possessing mcr-1 from a chicken meat sample was relatively high, at 38.3% [25]. However, these detection rates were lower than those found in the present study (66.7%).…”

Section: Discussioncontrasting

confidence: 84%

Quantitative Analysis of Colistin-Resistant Escherichia Coli in Retail Meat from Local Vietnamese Markets

Nguyen

Khong

et al. 2020

Preprint

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Objective: The spread of drug-resistant bacteria via food has contributed to the dissemination of resistant bacteria among humans. However, the status of food contamination with resistant bacteria, particularly the level of resistant bacteria in food, have not yet been well elucidated.Results: In this study, the abundance of colistin-resistant Escherichia coli in meat samples was quantified to understand the origin of the contamination of meat available in local Vietnamese markets. Fifteen samples each of chicken and pork meat purchased from local Vietnamese markets were assessed for the presence of colistin-resistant E. coli with the mobile colistin resistance gene mcr. The results showed that 40% (6/15) and 66% (10/15) of the pork and chicken meat samples, respectively, were contaminated with colistin-resistant E. coli. The median levels of colistin-resistant E. coli in the contaminated pork and chicken samples were 1.8 × 104 and 4.2 × 103 CFU/g, respectively. The results of phylogenetic analysis of isolates from a chicken meat sample showed that the contaminated colistin-resistant E. coli were a mix of multiple phylogenetical clones of bacteria that may have multiplied during sale. This is the first study to quantify the abundance of colistin-resistant E. coli in meat samples.

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Section: Discussioncontrasting

confidence: 84%

Quantitative Analysis of Colistin-Resistant Escherichia Coli in Retail Meat from Local Vietnamese Markets

Nguyen

Khong

et al. 2020

Preprint

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“…Similarly, other studies have shown that the detection rate of E. coli possessing mcr-1 from chicken meat samples collected from Germany [19], China [2], Japan [22], Brazil [23], and Nepal [24] ranged from 1.4% to 19.5%. More recently, a study in Tunisia showed that the rate of E. coli possessing mcr-1 from a chicken meat sample was relatively high, at 38.3% [25]. However, these detection rates were lower than those found in the present study (66.7%).…”

Section: Discussioncontrasting

confidence: 84%

Quantitative Analysis of Colistin-Resistant Escherichia Coli in Retail Meat from Local Vietnamese Markets

Nguyen

Khong

et al. 2020

Preprint

View full text Add to dashboard Cite

The spread of drug-resistant bacteria via food has contributed to the dissemination of resistant bacteria among humans. However, the status of food contamination with resistant bacteria, particularly the quantitative level of resistant bacteria in food, has not yet been well elucidated. In this study, the abundance of colistin-resistant Escherichia coli in meat samples was quantified to understand the origin of the contamination of meat available in local Vietnamese markets. Fifteen samples each of chicken and pork meat purchased from local Vietnamese markets were assessed for the presence of colistin-resistant E. coli with the mobile colistin resistance gene mcr. The results showed that 40% (6/15) and 66% (10/15) of the pork and chicken meat samples, respectively, were contaminated with colistin-resistant E. coli. The median quantitative levels of colistin-resistant E. coli in the contaminated pork and chicken samples were 1.8 × 104 and 4.2 × 103 CFU/g, respectively. The results of phylogenetic analysis of isolates from a chicken meat sample showed that the contaminated colistin-resistant E. coli were a mix of multiple phylogenetical clones of bacteria that may have multiplied during sale. This is the first study to quantify the abundance of colistin-resistant E. coli in meat samples.

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“…The presence of the beta‐lactamase genes [ bla CTX‐M (groups 1 and 9), bla SHV , bla TEM , bla OXA‐1 , and bla OXA‐48 ] was studied by PCR and sequencing (Ruiz et al ., 2012; Hassen et al ., 2019). Furthermore, the mcr‐ 1 (colistin resistance), tet A/ tet B (tetracycline resistance) and int 1 genes (integrase of class 1 integrons) were also analysed (Hassen et al ., 2019). Virulence factors were also tested ( sxt 1,2 ) (Alonso et al ., 2017).…”

Section: Methodsmentioning

confidence: 99%

Molecular diversity of Extended‐spectrum β‐lactamase‐producing Escherichia coli from vultures in Canary Islands

Carvalho

Tejedor-Junco

González-Martín

et al. 2020

Environ Microbiol Rep

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Antimicrobial resistance among isolates from wild animals is increasingly reported. Extended‐spectrum beta‐lactamase (ESBL)‐producing Enterobacteriaceae, and particularly Escherichia coli, have spread worldwide as one of the most common multidrug‐resistant organisms. The aim of this study was to determine the carriage rate of ESBL‐producing E. coli isolates and their genetic characteristics in wild vultures from the Canary Islands. Faecal samples were collected from 22 apparently healthy free‐ranging (wild) vulture chicks from Lanzarote and Fuerteventura (Canary Islands) during July 2019. They were seeded in MacConkey agar supplemented with cefotaxime (2 μg ml−1). Colonies with typical morphology of E. coli were identified by MALDI‐TOF‐MS. Antimicrobial susceptibility was done by disk diffusion. Phenotypic detection of ESBL was performed by double‐disk tests. The presence of blaCTX‐M, blaSHV, blaTEM, blaKPC and blaOXA‐48 genes, as well as mcr‐1 (colistin resistance), tetA/tetB and int1 gene, was tested by PCR/sequencing. Phylogenetic groups and multilocus sequence typing (MLST) were determined by PCR/sequencing. ESBL‐producing E. coli isolates were detected in 5/22 tested animals (22.7%), and all isolates (one/animal) carried blaCTX‐M genes: blaCTX‐M‐15 (n = 3) and blaCTX‐M‐55 (n = 2). ESBL‐positive isolates were ascribed to phylogenetic group D (two isolates), B1 (two isolates) and A (one isolate), and five sequence types were detected (ST/phylogenetic‐group/ESBL): ST515/B1/CTX‐M‐15, ST1290/A/CTX‐M‐15, ST38/D/CTX‐M‐15, ST457/D/CTX‐M‐55 and ST6448/B1/CTX‐M‐55; this suggests a genetic diversity among these isolates. Three CTX‐M‐15‐producing isolates contained the blaTEM gene and one the tetA gene. To our knowledge, this appears to be the first report of ESBL‐producing E. coli in vulture chicks from the Canary Islands.

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High prevalence of mcr-1 encoding colistin resistance and first identification of blaCTX-M-55 in ESBL/CMY-2-producing Escherichia coli isolated from chicken faeces and retail meat in Tunisia

Cited by 64 publications

References 40 publications

Quantitative Analysis of Colistin-Resistant Escherichia Coli in Retail Meat from Local Vietnamese Markets

Quantitative Analysis of Colistin-Resistant Escherichia Coli in Retail Meat from Local Vietnamese Markets

Quantitative Analysis of Colistin-Resistant Escherichia Coli in Retail Meat from Local Vietnamese Markets

Molecular diversity of Extended‐spectrum β‐lactamase‐producing Escherichia coli from vultures in Canary Islands

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