High prevalence of imipenem-resistant and metallo-beta-lactamase-producing Pseudomonas aeruginosa in the Burns Hospital in Tunisia: detection of a novel class 1 integron

Chairat, Sarra; Yahia, Houssem Ben; Rojo–Bezares, Beatriz; Sáenz, Yolanda; Torres, Carmén; Slama, Karim Ben

doi:10.1080/1120009x.2019.1582168

Cited by 24 publications

(29 citation statements)

References 34 publications

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“…24 In contrast, this rate in Tunisia and Nigeria were >50% of their collected isolates. 25,26 However, our results about the frequency of class 1 integrons among MDR P. aeruginosa isolates (63.4%) were comparable with a similar study carried out in Egypt (61.1%). 27 This difference may be due to the different policies and management of antibiotics for the treatment of infections in the world.…”

Section: Discussionsupporting

confidence: 86%

Distribution and Molecular Characterization of Resistance Gene Cassettes Containing Class 1 Integrons in Multi-Drug Resistant (MDR) Clinical Isolates of Pseudomonas aeruginosa

Ahmadian¹,

Haghshenas²,

Mirzaei³

et al. 2020

IDR

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The integrons, as the mobile exogenous elements, play a prominent role in the spreading of antimicrobial resistance genes from Pseudomonas aeruginosa clinical isolates to other bacteria. This study aimed to investigate the frequency of class 1 integrons andresistance gene cassettes carrying by them in clinical isolates as well as multidrug resistant P. aeruginosa. Materials and Methods: A total of 100 clinical isolates of P. aeruginosa were collected from 5 hospitals in Mazandaran province, north Iran. The antibiotic susceptibility pattern of the isolates was evaluated using the disk agar diffusion method. Genomic DNAs were extracted and then the presence of class 1 integrons was detected by the PCR test. All PCR products of the positive isolates were sequenced for the detection of resistance gene cassettes by the Sanger method. Results: Forty-one percent of the clinical isolates were multi-drug resistant. Also, 42% of the isolates were contained class 1 integron, and 61.9% of the integron positive isolates were detected as MDR. We detected 10 different gene cassettes sizing from 0.6 to 3.5 kb in the present study. The sequencing analysis of the internal variable regions of the class 1 integrons showed that the 0.75 kb gene cassette (aadB) was the most frequent resistance gene (54.76%) among all clinical isolates, as well as the MDR isolates. Other resistance genes detected in this study were included: aadA6-orfD (35.71%), aacA4-bla OXA-10 (21.42%), aadB-aacA4-bla OXA-10 (19.04%), bla OXA-10-aacA4-VIM1 (11.9%), aacA4-cat B10 (7.14%), aacA5-aadA1-cmlA5 (7.14%), bla OXA31-aadA2 (4.76%), and aac(3)-Ic-aac A5-cmlA5 (4.76%). To the best of our knowledge, bla OXA-10-aacA4-VIM1 cassette array is detected for the first time in this study. Conclusion: The treatment of infections caused by P. aeruginosa in this region of Iran is a major problem due to the high prevalence of class 1 integrons. It seems that the high prescription of beta-lactams and aminoglycosides for the treatment of these infections may be replaced by other combination therapy stewardships.

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Section: Discussionsupporting

confidence: 86%

Distribution and Molecular Characterization of Resistance Gene Cassettes Containing Class 1 Integrons in Multi-Drug Resistant (MDR) Clinical Isolates of Pseudomonas aeruginosa

Ahmadian¹,

Haghshenas²,

Mirzaei³

et al. 2020

IDR

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“…In fact, owe to its outer membrane low permeability, expression of efflux pumps, and biofilm formation, P. aeruginosa is one of the most difficult organisms to treat with antibiotics. Skin infections by P. aeruginosa on burn victims are particularly problematic, in part due to non-efficient treatments (35–37). The World Health Organization (WHO) included carbapenem resistant P. aeruginosa in a list of critical priority bacteria to which the research and development of new antibiotics and alternative treatments is of paramount importance (38).…”

Section: Introductionmentioning

confidence: 99%

Visible Light Plasmon Excitation of Silver Nanoparticles Against Antibiotic-ResistantPseudomonas aeruginosa

Silva

Petri

Valencia

et al. 2020

Preprint

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17The interaction of metallic nanoparticles with light excites a local surface plasmon resonance 18 (LSPR). This phenomenon enables the transfer of hot electrons to substrates that release 19 Reactive Oxygen Species (ROS). In this context, the present study was aimed at enhancing 20 the antibacterial effect of citrate-covered silver nanoparticles (AgNPs), which already possess 21 excellent antimicrobial properties, via LSPR excitation with visible LED 22 against Pseudomonas aeruginosa, one of the most refractory organisms to antibiotic 23 treatment. The Minimum Inhibitory Concentration (MIC) of AgNPs was 10 μg/ml under dark 24 conditions and 5 μg/ml under light conditions. The combination of light and AgNPs led to 25 100% cell death after 60 minutes. Quantification of ROS via flow cytometry showed that 26 LSPR stimulated AgNPs increased intracellular ROS concentration by 4.8-fold, suggesting 27 that light-exposed AgNPs caused cell death via ROS production. Light exposition caused a 28 small release of silver ions (0.4%) reaching a maximum after 6 hours. This indicates that 29 silver ions play at most a secondary role in P. aeruginosa death. Overall, the results presented 30 here show that LSPR generation from AgNPs by visible light enhances the antimicrobial 31 activity of silver nanoparticles and can be an alternative for the treatment of topic infections 32 caused by antibiotic-resistant bacteria such as P. aeruginosa. 33 34 36 37 Running title: Light-enhanced Ag nanoparticles against P. aeruginosa 38 39 40 42 ability of fine tuning their composition, structure and shape (1). The desired electronic, 43 optical and chemical properties of nanoparticles can be obtained through modern synthetic 44 techniques devised in the past decades (2). Even small variations in one of these parameters 45 may result in new properties that are absent in the analogous bulk material. Due to its high 46 range of controllable features, metallic nanoparticles are now used in many processes, such as 47 48others. 49Silver nanoparticles (AgNPs)(2), exhibit a great potential of use in many areas, due to their 50 relative easy and controlled synthesis (8), ranging from small and simple spheroidal AgNPs 51 (9) to more complex geometries (10, 11) and even asymmetric shapes (12-14) with solid or 52 hollow interiors (15-17). Historrically, silver has been extensively used as an antimicrobial 53 agent. Many different commercial products relying on silver biocidal properties have been 54 produced, most of them in the last 10 years (18, 19). The wide variety of AgNPs as well as 55 the intrinsic properties of metallic silver in relation to biological system and biomolecules 56 (20) fostered the extensive use of AgNPs in medical applications, such as cancer treatment 57 (21-23), drug delivery (24, 25), imaging (26, 27) and antimicrobial treatment (28, 29). 58 P. aeruginosa is a ubiquitous Gram-negative γ-proteobacterium and an opportunistic 59 pathogen associated with nosocomial infections. It also affects patients afflicted by chronic 60 res...

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“…Class 2 integrons are related to Tn7 transposon and associated with resistance to trimethoprim, streptomycin and spectinomycin, while class 3 integrons are rare (10). In recent years, the role of integrons has been identified as a genetic element in the horizontal transfer of antibiotic resistance genes (11)(12)(13)(14)(15). Integrons play a key role in the expression and spread of antibiotic resistance genes (16)(17)(18).…”

Section: Introductionmentioning

confidence: 99%

Determination Antimicrobial Resistance Profile and Prevalence of Class 1 and 2 Integron Resistance Gene Cassettes in Pseudomonas aeruginosa Strains Isolated from Hospitalized Patients in Markazi Province, Iran

Shojapour

Mirnezami

Ghaznavi‐Rad

et al. 2020

Jundishapur J Microbiol

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Background: Pseudomonas aeruginosa is recognized as a serious opportunistic pathogen that causes infections in hospitalized patients and shows a high level of antibiotic resistance. Objectives: The current study aimed at investigating the frequency of class 1, 2 integrons in P. aeruginosa strains isolated from hospitalized patients in Markazi Province, Iran. Methods: Totally, 100 clinical isolates of P. aeruginosa were collected and identified using standard biochemical tests P. aeruginosa. Antibiotic sensitivity test was performed using disk-diffusion (Bauer-Kirby) method. DNA was extracted, then polymerase chain reaction (PCR) was performed for the detection of class 1 and 2 integrons. Results: Trimethoprim/sulfamethoxazole (76%) and cefotaxime (45%) were the most effective antimicrobial agents in this study. Also, PCR results showed that 95% of P. aeruginosa strains carried int1, but none of the isolates harbored the int2 genes. A significant correlation was observed between class 1 integrons and resistance to trimethoprim/sulfamethoxazole and cefotaxime (P < 0.005). Conclusions:The current study recognized a high prevalence of antibiotic resistance also a high presence of class 1 integron. Since integron-carrying elements are responsible for the transmission of antibiotic resistance genes introduced into the hospital is of concern; thus applying comprehensive strategies for prevention and control of infections is necessary.

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High prevalence of imipenem-resistant and metallo-beta-lactamase-producing Pseudomonas aeruginosa in the Burns Hospital in Tunisia: detection of a novel class 1 integron

Cited by 24 publications

References 34 publications

<p>Distribution and Molecular Characterization of Resistance Gene Cassettes Containing Class 1 Integrons in Multi-Drug Resistant (MDR) Clinical Isolates of <em>Pseudomonas aeruginosa</em></p>

<p>Distribution and Molecular Characterization of Resistance Gene Cassettes Containing Class 1 Integrons in Multi-Drug Resistant (MDR) Clinical Isolates of <em>Pseudomonas aeruginosa</em></p>

Visible Light Plasmon Excitation of Silver Nanoparticles Against Antibiotic-ResistantPseudomonas aeruginosa

Determination Antimicrobial Resistance Profile and Prevalence of Class 1 and 2 Integron Resistance Gene Cassettes in Pseudomonas aeruginosa Strains Isolated from Hospitalized Patients in Markazi Province, Iran

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