High prevalence of <i>cfxA</i>β‐lactamase in aminopenicillin‐resistant <i>Prevotella</i> strains isolated from periodontal pockets

Fosse, T.; Madinier, I.; Hannoun, L.; Giraud‐Morin, C.; Hitzig, C; Charbit, Yves; Ourang, S.

doi:10.1046/j.0902-0055.2001.00096.x

Cited by 48 publications

(52 citation statements)

References 22 publications

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“…In this study, the detection of ␤-lactamase was limited to Prevotella strains (Table 1). Other studies have revealed that ␤-lactamase-positive strains of facultative anaerobic bacteria such as staphylococci and non-Prevotella anaerobes are also isolated from dental infections (4,7,16). However, even in these previous studies, the incidence of such bacteria and the prevalence of ␤-lactamase-positive strains in these species were very low.…”

Section: Discussioncontrasting

confidence: 41%

“…␤-Lactamase produced by Prevotella strains has the properties of class A-group 2e ␤-lactamases, which hydrolyze most peni-cillins and broad-spectrum cephalosporins but are inactive with respect to penicillin combined with clavulanic acid and imipenem (3). Although the genetic basis of ␤-lactamase-production by Prevotella strains has not been clarified completely, it has been demonstrated that the cfxA2 gene is associated with ␤-lactamase production (4,6,19). This gene shares 98% identity with cfxA, the structural gene of a ␤-lactamase produced by Bacteroides vulgatus (19).…”

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confidence: 99%

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Detection of cfxA and cfxA2 , the β-Lactamase Genes of Prevotella spp., in Clinical Samples from Dentoalveolar Infection by Real-Time PCR

et al. 2006

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While most bacteria involved in dentoalveolar infection are highly susceptible to penicillin, some Prevotella strains exhibit resistance to this agent through the production of ␤-lactamase. The production of ␤-lactamase by Prevotella spp. is in turn associated with the expression of the genes cfxA and cfxA2. The aim of the present study was to determine the prevalence of cfxA and cfxA2 in Prevotella strains by use of real-time PCR and to assess the performance of this molecular method for the direct detection of the genes in 87 clinical samples (pus and root canal exudates) from dentoalveolar infection. Production of ␤-lactamase by each isolate was determined using a nitrocefin disk. ␤-Lactamase production was seen in 31% of Prevotella isolates, while all isolates of other species were ␤-lactamase negative. The penicillin resistance of isolates strongly correlated with the production of ␤-lactamase. Real-time PCR was found to detect the cfxA and cfxA2 genes from at least five cells per reaction mixture (5 ؋ 10 3 CFU/ml of pus). Using real-time PCR, the presence of cfxA and cfxA2 was evident for all 48 ␤-lactamase-positive Prevotella strains. In contrast, neither ␤-lactamase-negative Prevotella (n ‫؍‬ 91) or non-Prevotella (n ‫؍‬ 31) strains were positive for the genes. In this study, 31 of the 87 samples yielded ␤-lactamase-positive Prevotella results, and cfxA and cfxA2 were detected in all 31 samples. Of the 56 culture-negative samples, 8 (14%) were positive for cfxA and cfxA2 by the real-time PCR. This sensitive and specific molecular method offers a rapid clinical test for aiding in the selection of an appropriate antibiotic for treatment of dentoalveolar infection. Although penicillin remains largely effective in the treatment of dentoalveolar infection, ␤-lactamase-stable antibiotics should be considered in cases in which ␤-lactamase-positive Prevotella strains are involved.

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Section: Discussioncontrasting

confidence: 41%

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Detection of cfxA and cfxA2 , the β-Lactamase Genes of Prevotella spp., in Clinical Samples from Dentoalveolar Infection by Real-Time PCR

et al. 2006

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“…In addition, microorganisms producing ß-lactamases are frequently detected in dental biofilm from childhood to adulthood particularly in infectious processes (Fosse et al, 2002;Kuriyama et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Susceptibility to ß-lactams and Metronidazole of Microorganisms Isolated from Chronic and Aggressive Periodontitis

Júnior

Schweitzer

2013

Int. J. Odontostomat.

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susceptibility to ß-lactams and metronidazole of microorganisms isolated from chronic and aggressive periodontitis. Int. J. Odontostomat., 7(2):193-198, 2013. ABSTRACT:The objective of this study was to evaluate the microbial susceptibility to ß-lactams and metronidazole, and evaluate the production of ß-lactamases by microorganisms isolated from patients with chronic or aggressive periodontitis. The samples were obtained from 50 patients with periodontitis and microorganisms were isolated onto selective and nonselective culture media, identified by biochemical methods and tested for susceptibility to antimicrobial agents (amoxicillin, amoxicillin/clavulanate, cefoxitin, imipenem, metronidazole, penicillin G). The isolates were resistant to at least 1 mg/ml of any drug tested were evaluated to verify the production of ß-lactamases by the method of double layer (or biological) and chromogenic cephalosporin using nitrocefin. The results evidenced resistance to amoxicillin and penicillin G, while the susceptibility to association amoxicillin/clavulanate, imipenem and cefoxitin was widely disseminated among the organisms. Resistance to these drugs showed a clear correlation with the production of ß-lactamase in the majority of microbial groups.

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“…Les ß-lactamases sont mises en évidence chez Prevotella et Fusobacterium (Tab. 5) [8][9][10][11] . Au moins 30 % des Prevotella d'origine dentaire produisent une ß-lactamase.…”

Section: Sensibilité Des Anaérobies Aux Antibiotiquesunclassified

Arguments microbiologiques pour optimiser l’antibiothérapie empirique des cellulites cervico-faciales

Dubreuil¹,

Neut²

2005

Med Buccale Chir Buccale

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RÉSUMÉLe traitement des cellulites vise à éradiquer streptocoques et anaérobies stricts. Lors de traumatismes ou dans les cellulites cutanées, il faudra prendre en compte les staphylocoques. Dans les infections plus sévères, le rôle des entérobactéries est discuté et souvent considéré comme acquis. Dans des situations extrêmes, sur documentation bactériologique, on peut être confronté à la présence de Pseudomonas aeruginosa ou de Staphylococcus aureus méthicilline-résistant. L'évolution de la résistance des anaérobies stricts à l'amoxicilline (production de ß-lactamase) ou à la clindamycine doit être régulièrement suspectée. Dans les infections sévères des associations, notamment avec les aminoglycosides, sont préconisées. Dans les cellulites circonscrites d'origine dentaire, les associations amoxicilline + métronidazole ou spiramycine + métronidazole sont envisageables. Pour les cellulites cutanées d'extension modérée, on peut utiliser oxacilline + métronidazole, clindamycine ± métronidazole, amoxicilline + acide clavulanique. Lorsque la cellulite est sévère amoxicilline + acide clavulanique, pipéracilline + tazobactam, céfotaxime ou ceftriaxone + métronidazole sont souvent associés aux aminoglycosides. S'il y a un risque pour le pronostic vital, c'est l'association imipénème + aminoglycoside qui sera retenue.

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High prevalence of cfxAβ‐lactamase in aminopenicillin‐resistant Prevotella strains isolated from periodontal pockets

Cited by 48 publications

References 22 publications

Detection of cfxA and cfxA2 , the β-Lactamase Genes of Prevotella spp., in Clinical Samples from Dentoalveolar Infection by Real-Time PCR

Detection of cfxA and cfxA2 , the β-Lactamase Genes of Prevotella spp., in Clinical Samples from Dentoalveolar Infection by Real-Time PCR

Antimicrobial Susceptibility to ß-lactams and Metronidazole of Microorganisms Isolated from Chronic and Aggressive Periodontitis

Arguments microbiologiques pour optimiser l’antibiothérapie empirique des cellulites cervico-faciales

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