High Prevalence of Genetically Related Clostridium Difficile Strains at a Single Hemato-Oncology Ward Over 10 Years

Waker, Edyta; Ambrożkiewicz, Filip; Kulecka, Maria; Paziewska, Agnieszka; Skubisz, Karolina; Cybula, Patrycja; Targoński, Łukasz; Mikula, Michał; Walewski, Jan; Ostrowski, Jerzy

doi:10.3389/fmicb.2020.01618

Cited by 4 publications

(2 citation statements)

References 87 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As reported recently [70], between 2008 and 2018, all patients hospitalized at the Department of Lymphoma with healthcare-associated diarrhea (defined as ≥3 stools within a 24-h period arising over the third day after hospital admission) underwent testing at the Department of Clinical Microbiology to detect pathogenic C. difficile toxins A and B. Tests were performed using the C. difficile TOX A/B kit (TechLab).…”

Section: Methodsmentioning

confidence: 99%

Higher genome variability within metabolism genes associates with recurrent Clostridium difficile infection

et al. 2021

Self Cite

View full text Add to dashboard Cite

Background Clostridium difficile (C. difficile) is a major source of healthcare-associated infection with a high risk of recurrence, attributable to many factors such as usage of antibiotics, older age and immunocompromised status of the patients. C. difficile has also a highly diverse genome, which may contribute to its high virulence. Herein we examined whether the genome conservation, measured as non-synonymous to synonymous mutations ratio (dN/dS) in core genes, presence of single genes, plasmids and prophages increased the risk of reinfection in a subset of 134 C. difficile isolates from our previous study in a singly hemato-oncology ward. Methods C. difficile isolates were subjected to whole-genome sequencing (WGS) on Ion Torrent PGM sequencer. Genomes were assembled with MIRA5 and annotated with prokka and VRprofile. Logistic regression was used to asses the relationship between single gene presence and the odds of infection recurrence. DN/dS ratios were computed with codeml. Functional annotation was conducted with eggNOG-Mapper. Results We have found that the presence of certain genes, associated with carbon metabolism and oxidative phosphorylation, increased the odds of infection recurrence. More core genes were under positive selective pressure in recurrent disease isolates – they were mostly associated with the metabolism of aminoacids. Finally, prophage elements were more prevalent in single infection isolates and plasmids did not influence the odds of recurrence. Conclusions Our findings suggest higher genetic plasticity in isolates causing recurrent infection, associated mainly with metabolism. On the other hand, the presence of prophages seems to reduce the isolates’ virulence.

show abstract

Section: Methodsmentioning

confidence: 99%

Higher genome variability within metabolism genes associates with recurrent Clostridium difficile infection

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…For instance, a study by Walker et al analysed 41 recurrent episodes in cancer patients using WGS and found that 29% of these episodes were caused by a different strain, indicating reinfection. However, when recurrence occurred within the first 2 months after the initial CDI, it was always caused by the original strain [47]. The study also revealed a high prevalence of genetically related strains, suggesting possible nosocomial transmission, although this aspect was not specifically addressed.…”

Section: Andandmentioning

confidence: 86%

Prevention and treatment of C. difficile in cancer patients

Puerta-Alcalde,

Garcia-Vidal,

Soriano

2023

Current Opinion in Infectious Diseases

View full text Add to dashboard Cite

Purpose of review We provide an update on the recent literature on Clostridioides difficile infection (CDI) in cancer patients. Recent findings Distinguishing between colonization and infection remains challenging in cancer patients. Many patients with negative toxin analysis are still treated for CDI, and some meet criteria for severe cases. The incidence of CDI is high in cancer patients, especially those with haematological malignancies. Disruption of the gut microbiome due to antibiotic consumption, chemotherapy and radiotherapy is the primary factor contributing to CDI development. The severity of CDI in cancer patients is often unclear due to the absence of well-defined severity criteria. Certain microbiome species predominance and specific ribotypes have been associated with worse outcomes. Whole genome sequencing could be helpful for differentiating recurrence from reinfection and exploring potential nosocomial transmission. While certain new drugs such as fidaxomicin or bezlotoxumab show promise, the optimal treatment and prevention strategies for CDI in cancer patients remain uncertain. Faecal microbiota transplantation (FMT) holds potential for reducing CDI recurrence rates. Summary Further studies are needed to provide robust recommendations for diagnosis, grading severity, and therapeutic management of CDI in cancer patients. Recurrences are particularly concerning due to subsequent exposition to CDI risk factors.

show abstract

Molecular characterization of Clostridioides difficile by multi-locus sequence typing (MLST): A study from tertiary care center in India

et al. 2022

View full text Add to dashboard Cite

High Prevalence of Genetically Related Clostridium Difficile Strains at a Single Hemato-Oncology Ward Over 10 Years

Cited by 4 publications

References 87 publications

Higher genome variability within metabolism genes associates with recurrent Clostridium difficile infection

Higher genome variability within metabolism genes associates with recurrent Clostridium difficile infection

Prevention and treatment of C. difficile in cancer patients

Molecular characterization of Clostridioides difficile by multi-locus sequence typing (MLST): A study from tertiary care center in India

Contact Info

Product

Resources

About