High prevalence of dual and triple viral infections in black tiger shrimp ponds in India

Umesha, Kanasinakatte R.; Dass, Bob Kennedy M.; Naik, B. M.; Venugopal, M. N.; Karunasagar, Indrani

doi:10.1016/j.aquaculture.2006.04.003

Cited by 36 publications

(25 citation statements)

References 22 publications

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“…Many shrimp specimens had dual or multiple infections with monodon baculovirus (MBV), heptopancreatic parvovirus (HPV) and infectious hypodermal and haematopoietic necrosis virus (IHHNV) (Chayaburakul et al ., 2004). After screening shrimp samples from 18 ponds in India, Umesha and colleagues (2006) found that the animals in seven ponds showed dual infections with WSSV and MBV and in 10 ponds triple infections with HPV, WSSV and MBV could be found. Selvin and Lipton (2003) demonstrated the presence of a virulent strain of V. alginolyticus in shrimp from a pond hit by a WSSV outbreak.…”

Section: Introductionmentioning

confidence: 99%

Increased susceptibility of white spot syndrome virus‐infected Litopenaeus vannamei to Vibrio campbellii

Phuoc

Corteel²,

Nauwynck³

et al. 2008

Environmental Microbiology

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SummaryThe concept of polymicrobial disease is well accepted in human and veterinary medicine but has received very little attention in the field of aquaculture. This study was conducted to investigate the synergistic effect of white spot syndrome virus (WSSV) and Vibrio campbellii on development of disease in specific pathogen-free (SPF) shrimp Litopenaeus vannamei. The juvenile shrimp were first injected with WSSV at a dose of 30 SID 50 shrimp -1 (SID50 = shrimp infectious dose with 50% endpoint) and 24 h later with 10 6 colony-forming units (cfu) of V. campbellii shrimp -1 . Controls receiving just one of the pathogens or negative inocula were included. In the treatment with WSSV only, shrimp started to die at 48-108 h post injection (hpi) and cumulative mortality reached 100% at 268-336 hpi. In the treatment with only V. campbellii injection (10 6 cfu shrimp), cumulative mortality reached 16.7%. Shrimp in the dual treatment died very quickly after V. campbellii injection and 100% cumulative mortality was obtained at 72-96 hpi. When WSSV-injected shrimp were given sonicated V. campbellii instead of live V. campbellii, no synergistic effect was observed. Density of V. campbellii in the haemolymph of co-infected moribund shrimp collected 10 h after V. campbellii injection was significantly higher than in shrimp injected with V. campbellii only (P < 0.01). However, there was no difference in WSSV replication between shrimp inoculated with WSSV only compared with dually inoculated ones. This study revealed that prior infection with WSSV enhances the multiplication and disease inducing capacity of V. campbellii in shrimp.

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Section: Introductionmentioning

confidence: 99%

Increased susceptibility of white spot syndrome virus‐infected Litopenaeus vannamei to Vibrio campbellii

Phuoc

Corteel²,

Nauwynck³

et al. 2008

Environmental Microbiology

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“…The present study provides an explanation for the occurrence of strains with a smaller number of repeat sizes in WSSV outbreaks. It is common to observe ponds where animals are apparently healthy, but are carrying WSSV (Umesha, Kennedy, Naik, Venugopal, Karunasagar & Karunasagar 2006;Pradeep et al 2008b). Study of repeat regions in viruses in such ponds may be useful in predicting the risk of outbreaks of disease.…”

mentioning

confidence: 99%

Fitness and virulence of different strains of white spot syndrome virus

Pradeep

Karunasagar

2009

Journal of Fish Diseases

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“…All the four ponds in the same farm were affected by multiple infections which were confirmed by PCR assay using pathogen‐specific primer sets. Multiple infections caused by different combinations of shrimp viruses have been reported by many workers in shrimp (Chayaburakul et al, ; Dewangan et al, ; Feijó et al, ; Flegel et al, ; Macías‐Rodríguez et al, ; Manivannan et al, ; Natividad et al, ; Otta et al, ; Senapin et al, ; Tan et al, ; Teixeira‐Lopes et al, ; Umesha et al, ; Yeh, Chen, Hsieh, Cheng, & Liu, ).…”

Section: Discussionmentioning

confidence: 96%

Multiple infections caused by white spot syndrome virus and Enterocytozoon hepatopenaei in pond‐reared Penaeus vannamei in India and multiplex PCR for their simultaneous detection

Thamizhvanan¹,

Sivakumar²,

Kumar³

et al. 2019

Journal of Fish Diseases

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White leg shrimp, Penaeus vannamei, were collected on a monthly basis from grow‐out ponds located at Tamil Nadu and Andhra Pradesh states along the east coast of India for screening of viral and other pathogens. Totally 240 shrimp samples randomly collected from 92 farms were screened for white spot syndrome virus (WSSV), infectious hypodermal and haematopoietic necrosis virus (IHHNV), infectious myonecrosis virus (IMNV) and Enterocytozoon hepatopenaei (EHP). The number of shrimp collected from shrimp farms ranged from 6 to 20 based on the body weight of the shrimp. All the shrimp collected from one farm were pooled together for screening for pathogens by PCR assay. Among the samples screened, 28 samples were WSSV‐positive, one positive for IHHNV and 30 samples positive for EHP. Among the positive samples, four samples were found to be positive for both WSSV and EHP, which indicated that the shrimp had multiple infections with WSSV and EHP. This is the first report on the occurrence of multiple infections caused by WSSV and EHP. Multiplex PCR (m‐PCR) protocol was standardized to detect both pathogens simultaneously in single reaction instead of carrying out separate PCR for both pathogens. Using m‐PCR assay, naturally infected shrimp samples collected from field showed two prominent bands of 615 and 510 bp for WSSV and EHP, respectively.

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High prevalence of dual and triple viral infections in black tiger shrimp ponds in India

Cited by 36 publications

References 22 publications

Increased susceptibility of white spot syndrome virus‐infected Litopenaeus vannamei to Vibrio campbellii

Increased susceptibility of white spot syndrome virus‐infected Litopenaeus vannamei to Vibrio campbellii

Fitness and virulence of different strains of white spot syndrome virus

Multiple infections caused by white spot syndrome virus and Enterocytozoon hepatopenaei in pond‐reared Penaeus vannamei in India and multiplex PCR for their simultaneous detection

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