High prevalence of antimicrobial‐resistant <i>Escherichia coli</i> from animals at slaughter: a food safety risk

Ramos, Sónia; Silva, Nuno; Caniça, Manuela; Capelo-Martínez, José Luis; Brito, Francisco; Igrejas, Gilberto; Poeta, Patrícia

doi:10.1002/jsfa.5814

Cited by 20 publications

(22 citation statements)

References 46 publications

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“…For example, a study conducted in Portugal, 192 E. coli isolates from 198 fecal samples were detected from rectal samples of healthy food animals at slaughter. These include pig (n = 66; 34.37%), sheep (n = 73; 38.02%) and cattle (n = 53; 27.60%) isolates (Ramos et al 2013). This reveals that occurrence of E. coli in cattle (n=19; 95%) and sheep samples (n=15; 75%) was higher in our findings than the findings by Ramos et al (2013).…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial susceptibility of large intestinal Escherichia coli isolates from cattle and sheep abattoir samples

2016

Res. Opin. Anim. Vet. Sci.

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Escherichia coli reside in gastrointestinal tracts of warm-blooded animals and can serve as an indicator organism for monitoring antibiotic resistance that is considered an important global issue. In this study, E. coli (n=49) were isolated from 40 samples of colon contents consisting from 20 cattle and 20 sheep carcasses and identified based on standard biochemical tests. The identification was also verified by chromogenic media. All isolates were tested against 13 antibiotic agents by disc diffusion method for determining antimicrobial susceptibilities. Double Disk Synergy test was applied for the detection of the ESBL producing isolates. Nearly all isolates were found to be susceptible to most of the antibiotics. The resistance to tested antibiotics was low; only 6 (12.24%) of 49 E. coli isolates were resistant to at least one antibiotic agent. Furthermore, different resistance rates to antibiotics were observed for cattle and sheep isolates. Among 28 isolates from cattle, the highest resistance levels were observed for trimethoprim-sulfamethoxazole (n=3; 6.12%), imipenem (n=2; 4.08%) and cefpodoxime (n=1; 3.57), while only ciprofloxacin resistance (n=2 9.52%) was observed among 21 isolates from sheep. This is the first report about imipenem resistant E. coli of animal origin from Turkey. Furthermore, no ESBL positive isolate was detected at all. In conclusion, continuous and strategic surveillance of antimicrobial resistant bacteria in livestock is essential to suppress further dissemination of these bacteria into Turkish society.

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Section: Discussionmentioning

confidence: 99%

Antimicrobial susceptibility of large intestinal Escherichia coli isolates from cattle and sheep abattoir samples

2016

Res. Opin. Anim. Vet. Sci.

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“…Moreover, E. coli resistance to major antibiotics is increasing in almost all countries, and the situation is getting worse with the emergence of bacterial strains that are resistant to several antibiotics at once (Piras et al, 2012). This multidrug resistance (MDR), or resistance to at least two classes of antimicrobial agents, is now commonly found in E. coli from humans and animals ( Jiang et al, 2011;Ramos et al, 2013;Von Baum and Marre, 2005). …”

Section: Introductionmentioning

confidence: 99%

“…These antimicrobial-resistant bacteria, and their genes of resistance, can easily be transmitted to humans through the food chain. Moreover, recent publications from European and non-European countries show that MDR E. coli strains are prevalent among poultry and pigs (de Verdier et al, 2012;Jiang et al, 2011;Ramos et al, 2013). The increasing prevalence of bacteria that are resistant to the most up-to-date arsenal of antibiotics is a serious concern for global public health, given the increased healthcare costs and mortality rates (Polyak et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…We selected 66 previously studied and characterized E. coli strains that had been recovered from fecal samples of pigs slaughtered for human consumption (Table 1) (Ramos et al, 2013). Of these 66 isolates, only 2 of them showed susceptibility to all the tested antibiotics, whereas 80% presented an MDR phenotype in some cases to antimicrobial agents belonging to at least three different categories (Ramos et al, 2013).…”

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Proteomics for Drug Resistance on the Food Chain? Multidrug-ResistantEscherichia coliProteomes from Slaughtered Pigs

Ramos

Silva

Hébraud

et al. 2016

OMICS: A Journal of Integrative Biology

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Understanding global drug resistance demands an integrated vision, focusing on both human and veterinary medicine. Omics technologies offer new vistas to decipher mechanisms of drug resistance in the food chain. For example, Escherichia coli resistance to major antibiotics is increasing whereas multidrug resistance (MDR) strains are now commonly found in humans and animals. Little is known about the structural and metabolic changes in the cell that trigger resistance to antimicrobial agents. Proteomics is an emerging field that is used to advance our knowledge in global health and drug resistance in the food chain. In the present proteomic analysis, we offer an overview of the global protein expression of different MDR E. coli strains from fecal samples of pigs slaughtered for human consumption. A full proteomic survey of the drug-resistant strains SU60, SU62, SU76, and SU23, under normal growth conditions, was made by two-dimensional electrophoresis, identifying proteins by MALDI-TOF/MS. The proteomes of these four E. coli strains with different genetic profiles were compared in detail. Identical transport, stress response, or metabolic proteins were discovered in the four strains. Several of the identified proteins are essential in bacterial pathogenesis (GAPDH, LuxS, FKBPs), development of bacterial resistance (Omp's, TolC, GroEL, ClpB, or SOD), and potential antibacterial targets (FBPA, FabB, ACC's, or Fab1). Effective therapies against resistant bacteria are crucial and, to accomplish this, a comprehensive understanding of putative resistance mechanisms is essential. Moving forward, we suggest that multi-omics research will further improve our knowledge about bacterial growth and virulence on the food chain, especially under antibiotic stress.

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“…In fact, intestinal commensal microflora acts as a potential reservoir of resistance genes that may be transferred to pathogenic bacteria inside the host. The diffusion of antimicrobial resistance among pathogens, that has been evidenced since the seventies, has resulted in a compromised efficacy of antimicrobial agents used in the treatment of infectious diseases, and is actually considered an emerging and serious public health concern (van den Boogard and Stobberingh, 2000;Martinez and Baquero, 2002;Ramos et al, 2013). In particular, the possibility of transferring antibiotic-resistant bacteria from food animals to humans through the consumption of meat or other animal products, by the contact with farm wastewater, and other ways must be regarded as a worldwide issue (Sàenz et al, 2004;Marshall and Levy, 2011).…”

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confidence: 99%

Effects of a species-specific probiotic formulation on multiresistant Escherichia coli isolates from the gut of veal calves

Ripamonti¹,

Tirloni²,

Stella³

et al. 2013

CZECH J ANIM SCI

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ABSTRACT:In this study, 254 Escherichia coli isolates from faecal samples of veal calves were evaluated for antimicrobial susceptibility using the disk diffusion method. During the experimental period, six mass antibiotic treatments were administered to the animals (about one treatment per month). The active principles used were oxytetracycline, colistin, tylosin, doxycycline, chlortetracycline, and sulphonamides. An extremely high resistance prevalence (> 70%) towards penicillin, sulphonamide, tetracycline, ampicillin, and spyramicin was detected. Sixty E. coli isolates could be defined as multiresistant, showing resistance to at least 6 antimicrobial classes. Subsequently, we evaluated the inhibitory effect of a species-specific probiotic against multiresistant E. coli, showing its beneficial action with large inhibition halos for 76% of the isolates. This suggests the potentiality of the probiotic, putting in evidence a clear advantage of its use in veal calves nutrition, in particular during the first phases, when the animals are more susceptible to severe enteric infections by E. coli.

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High prevalence of antimicrobial‐resistant Escherichia coli from animals at slaughter: a food safety risk

Abstract: Our data shows a high percentage of antibiotic resistance in E. coli isolates from food animals, and raises important questions in the potential impact of antibiotic use in animals and the possible transmission of resistant bacteria to humans through the food chain.

Cited by 20 publications

References 46 publications

Antimicrobial susceptibility of large intestinal Escherichia coli isolates from cattle and sheep abattoir samples

Antimicrobial susceptibility of large intestinal Escherichia coli isolates from cattle and sheep abattoir samples

Proteomics for Drug Resistance on the Food Chain? Multidrug-ResistantEscherichia coliProteomes from Slaughtered Pigs

Effects of a species-specific probiotic formulation on multiresistant Escherichia coli isolates from the gut of veal calves

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