High prevalence of antibodies against Chlamydiaceae and Chlamydophila abortus in wild ungulates using two “in house” blocking-ELISA tests

Salinas, J.; Caro, M.R.; Vicente, Joaquı́n; Cuello, F.; Reyes-Garcia, A.R.; Buendía, A. J.; Rodolakis, Annie; Gortázar, Christian

doi:10.1016/j.vetmic.2008.10.001

Cited by 40 publications

(26 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Uncultured Chlamydiales spp. were identified from 2 roe deer, 1 fallow deer, and 1 red deer, with less than 5 genome copies detected (Ct: 45.88-49.25; 17,21 In conclusion, the results of the present study show that Q fever is widespread among domestic ruminants in Hungary and, in some cases, this bacterium might be the primary cause of abortions. High rates of infections with both C. burnetii and C. abortus were detected in sheep.…”

mentioning

confidence: 50%

Occurrence of Coxiella burnetii and Chlamydiales species in abortions of domestic ruminants and in wild ruminants in Hungary, Central Europe

et al. 2015

View full text Add to dashboard Cite

Coxiella burnetii and certain members of the Chlamydiales order are zoonotic, intracellular, Gram-negative bacteria, with abortigenic potential in ruminants. These pathogens have a broad host range and worldwide geographical distribution. The current study aimed to reveal the importance of C. burnetii and Chlamydiales spp. in abortions in domestic ruminants and their occurrence in wild ruminants with real-time polymerase chain reaction (qPCR) assays, histology, and immunohistochemical staining (IHC). From the 111 abortion cases of domestic ruminants examined, C. burnetii was detected in 33 placenta samples (cattle, n = 22; sheep, n = 10; goat, n = 1), and members of the Chlamydiales order were detected in 32 placenta samples (cattle, n = 14; sheep, n = 16; goat, n = 2) using qPCR. Coinfection with both C. burnetii and Chlamydiales spp. were identified in 12 cases (cattle, n = 3; sheep, n = 8; goat, n = 1) out of the qPCR-positive samples. The presence of the relevant antigen was confirmed by IHC in 20 cases ( C. burnetii, n = 2, in sheep; Chlamydiaceae, n = 17, in sheep [ n = 15] and goat [ n = 2]; and both pathogens in 1 sheep). Coxiella burnetii was identified in 2.2% (2/91) of the wild ruminants, but the samples were negative by IHC. Uncultured Chlamydiales spp. were detected in 4.4% (4/91) of the placenta samples by qPCR. In conclusion, Q fever is widespread among domestic ruminants in Hungary, and, in several cases, C. burnetii was implicated as the primary cause of abortions. Waddlia chondrophila, Parachlamydia spp., and uncultured Chlamydiales spp. were present only sporadically in samples from cattle and wild ruminants.

show abstract

mentioning

confidence: 50%

Occurrence of Coxiella burnetii and Chlamydiales species in abortions of domestic ruminants and in wild ruminants in Hungary, Central Europe

et al. 2015

View full text Add to dashboard Cite

show abstract

“…This test is recommended by the Office Internationale des Epizooties for diagnosing chlamydiosis, but it is not species-specific because the antigen used (lipopolysaccharide from the chlamydial cell wall) is common to all Chlamydia species. Using two in-house blocking enzyme-linked immunosorbent assays, Salinas et al (2009) differentiated the antibody response to C. abortus from that to other Chlamydiaceae in wildlife hosts, suggesting frequent contacts with Chlamydiaceae other than C. abortus, such as C. pecorum, C. suis, and C. psittaci. We evaluated the prevalence of antibody against chlamydiae in a red deer (Cervus elaphus) population in Italy by the microimmunofluorescence (MIF) test; MIF is more specific than CFT (Saikku, 1982) because it shows antibody reactivity against genus-specific and species-specific antigens, by using purified elementary bodies as antigen.…”

Section: Introductionmentioning

confidence: 99%

Chlamydiosis: Seroepidemiologic Survey in a Red Deer (Cervus elaphus) Population in Italy

Francesco

Donati

Nicoloso

et al. 2012

Journal of Wildlife Diseases

View full text Add to dashboard Cite

ABSTRACT:Chlamydiae are obligate, intracellular, gram-negative bacteria that are responsible for important diseases in humans, other mammals, and birds. Studies have shown that chlamydiae could be present in wild ruminants, but the serodiagnostic method most commonly used did not allow identification of chlamydial species. We determined the prevalence of antibodies to Chlamydia pecorum, Chlamydia suis, Chlamydia abortus, and Chlamydia psittaci in 271 red deer (Cervus elaphus) of a central Italian population, by using the microimmunofluorescence test that shows antibody response against genus-specific and species-specific antigens. No sera had detectable antibodies to C. pecorum and C. abortus. Antibodies were detected against C. psittaci (9.6%) and C. suis (3.3%). Antibody response could be related to contact of the red deer with birds and wild boars (Sus scrofa), respectively, and confirm an extended host range of individual Chlamydia species. In view of the potential zoonotic risk related to exposition of C. psittaci, our findings suggest surveillance of wild ruminants as potential reservoirs for chlamydiae.

show abstract

“…Antibodies against viral pathogens include bovine herpesvirus 1, encephalomyocarditis virus (Gentile et al 2000), bovine parainfluenza type 3 virus, bovine respiratory syncytial virus (Gaffuri et al 2006), and pestivirus (Gentile et al 2000;Arnal et al 2004;Hurtado et al 2004;Gaffuri et al 2006;Marco et al 2007;Pioz et al 2007;Marco et al 2008). Regarding bacterial agents, antibodies have been detected against Brucella melitensis (Garin-Bastuji et al 1990), Mycoplasma conjunctivae (Giacometti et al 2002;Gaffuri et al 2006), Salmonella enterica, Coxiella burnetii (Pioz et al 2008), Leptospira interrogans (Gentile et al 2000), and Chlamydophila abortus (Pioz et al 2008;Salinas et al 2009). Finally, antibodies against two protozoa, Neospora caninum and Toxoplama gondii, have also been reported (Gentile et al 2000;Gaffuri et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Prevalence of antibodies against selected agents shared between Cantabrian chamois (Rupicapra pyrenaica parva) and domestic goats

et al. 2009

View full text Add to dashboard Cite

Southern chamois (Rupicapra pyrenaica) share the habitat with domestic ungulates, and may, therefore, play a role in the epidemiology of shared agents. The objective of this study was to determine the seroprevalence for Brucella spp., Mycobacterium avium ssp. paratuberculosis (MAP), pestivirus, and Sarcoptes scabiei in Cantabrian chamois (Rupicapra pyrenaica parva) and compare these data with those of sympatric domestic goats (Capra hircus). From 2005 to 2008, blood samples were obtained from 236 adult Cantabrian chamois in two different populations, the western one and the eastern one. Seroprevalence for Brucella spp. and pestivirus was assessed using commercial ELISA kits, whereas specifically designed ELISA tests were used for MAP and S. scabiei. No antibodies against Brucella spp. were detected. Conversely, antibodies against MAP, pestivirus (chamois 3.8%; goat 2.3%), and S. scabiei (chamois 11.9%; goat 12.8%) were detected in both species. Seroprevalence for MAP was significantly higher for domestic goats (26%) than for chamois (9.7%). In chamois, seroprevalence for pestivirus was higher in the west (6.5%) than in the east (range 0-1.8%), whereas seroprevalence for S. scabiei followed the opposite trend (west 4.6%; east 16.7-21.4%). We suggest that certain diseases could circulate between Cantabrian chamois and domestic goat populations, and domestic livestock may suppose a threat for the health status of sympatric Cantabrian chamois.

show abstract

High prevalence of antibodies against Chlamydiaceae and Chlamydophila abortus in wild ungulates using two “in house” blocking-ELISA tests

Cited by 40 publications

References 36 publications

Occurrence of Coxiella burnetii and Chlamydiales species in abortions of domestic ruminants and in wild ruminants in Hungary, Central Europe

Occurrence of Coxiella burnetii and Chlamydiales species in abortions of domestic ruminants and in wild ruminants in Hungary, Central Europe

Chlamydiosis: Seroepidemiologic Survey in a Red Deer (Cervus elaphus) Population in Italy

Prevalence of antibodies against selected agents shared between Cantabrian chamois (Rupicapra pyrenaica parva) and domestic goats

Contact Info

Product

Resources

About