High Prevalence and Widespread Distribution of Zoonotic <i>Enterocytozoon bieneusi</i> Genotypes in Swine in Northeast China: Implications for Public Health

Wan, Qiang; Lin, Yongchao; Mao, Yixian; Yang, Yuting; Li, Qiao; Zhang, Siwen; Jiang, Yanxue; Tao, Wei; Li, Wei

doi:10.1111/jeu.12264

Cited by 36 publications

(29 citation statements)

References 41 publications

(84 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These results differ from those determined by a survey conducted in Northeast China that showed the prevalence of E. bieneusi in preweaned pigs (58.0%, 94/162) was significantly higher than that in growing pigs (39.6%, 114/288) and weaned pigs (52.2%, 59/113; Wan et al. ). For Tibetan pigs and Yunan Black pigs, the preweaned and weaned piglets were subject to intensive feeding, while growing pigs transitioned to free‐range grazing.…”

Section: Discussioncontrasting

confidence: 96%

Multilocus Typing of Enterocytozoon bieneusi in Pig Reveals the High Prevalence, Zoonotic Potential, Host Adaptation and Geographical Segregation in China

Zheng

Zhou

et al. 2019

J Eukaryotic Microbiology

View full text Add to dashboard Cite

Enterocytozoon bieneusi is one of the most frequently diagnosed Microsporidia of humans and most animals. However, there is no information on E. bieneusi infection of pigs in Tibet and Henan, China. In this study, 1,190 fecal samples were collected from pigs in Tibet and Henan and screened for the presence of E. bieneusi. The overall prevalence of E. bieneusi infection was 54.2% (645/1,190), with differences in prevalence observed among geographical areas, ages, and pig breeds. Moreover, 10 E. bieneusi genotypes were identified based on internal transcribed spacer region genotyping, including eight known genotypes (EbpC, EbpA, CHG19, CHC5, Henan‐III, I, D, and H) and two novel genotypes (XZP‐I and XZP‐II). Multilocus sequence typing revealed 18, 7, 17, and 13 genotypes at minisatellite/microsatellite loci MS1, MS3, MS4, and MS7, respectively. Strong linkage disequilibrium (LD) and few numbers of recombination events, suggest a clonal structure of the E. bieneusi population examined in this study. The low pairwise genetic distance (FST) and gene flow (Nm) values indicated limited gene flow in the E. bieneusi population from different hosts, with phylogenetic, structure, and median‐joining network analyses all indicating the existence of host and geographical isolation. The identification of isolates belonging to nine human‐pathogenic genotypes indicates that pigs play an important role in the dissemination of E. bieneusi, improving our present understanding of E. bieneusi epidemiology in the studied region.

show abstract

Section: Discussioncontrasting

confidence: 96%

Multilocus Typing of Enterocytozoon bieneusi in Pig Reveals the High Prevalence, Zoonotic Potential, Host Adaptation and Geographical Segregation in China

Zheng

Zhou

et al. 2019

J Eukaryotic Microbiology

View full text Add to dashboard Cite

show abstract

“…The ITS genotypes of pig E . bieneusi isolates used for MLST analysis in this study all belong to phylogenetic Group 1 with zoonotic potential [2,17,23]. The ITS genotypes of primate E .…”

Section: Discussionmentioning

confidence: 99%

“…bieneusi were obtained from pigs in cities Changchun, Daqing, Harbin, and Qiqihar in northeast China. All of them were previously genotyped by PCR and sequence analysis of the ITS locus [17,29]. A total of 101 isolates of ITS genotypes CHN7, CS-4, EbpA, EbpB, EbpC, Henan-I, Henan-IV, O, and PigEBITS3 in zoonotic Group 1 were selected for population genetic analysis in this study.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Clonal Evolution of Enterocytozoon bieneusi Populations in Swine and Genetic Differentiation in Subpopulations between Isolates from Swine and Humans

et al. 2016

Self Cite

View full text Add to dashboard Cite

Enterocytozoon bieneusi is a widespread parasite with high genetic diversity among hosts. Its natural reservoir remains elusive and data on population structure are available only in isolates from primates. Here we describe a population genetic study of 101 E. bieneusi isolates from pigs using sequence analysis of the ribosomal internal transcribed spacer (ITS) and four mini- and microsatellite markers. The presence of strong linkage disequilibrium (LD) and limited genetic recombination indicated a clonal structure for the population. Bayesian inference of phylogeny, structural analysis, and principal coordinates analysis separated the overall population into three subpopulations (SP3 to SP5) with genetic segregation of the isolates at some geographic level. Comparative analysis showed the differentiation of SP3 to SP5 from the two known E. bieneusi subpopulations (SP1 and SP2) from primates. The placement of a human E. bieneusi isolate in pig subpopulation SP4 supported the zoonotic potential of some E. bieneusi isolates. Network analysis showed directed evolution of SP5 to SP3/SP4 and SP1 to SP2. The high LD and low number of inferred recombination events are consistent with the possibility of host adaptation in SP2, SP3, and SP4. In contrast, the reduced LD and high genetic diversity in SP1 and SP5 might be results of broad host range and adaptation to new host environment. The data provide evidence of the potential occurrence of host adaptation in some of E. bieneusi isolates that belong to the zoonotic ITS Group 1.

show abstract

“…) and many species of animals have been identified as reservoir hosts of this parasite (Wan et al. ; Yang et al. ; Ye et al.…”

mentioning

confidence: 99%

The Potential Role of Synanthropic Rodents and Flies in the Transmission of Enterocytozoon bieneusi on a Dairy Cattle farm in China

Zhao

et al. 2018

J Eukaryotic Microbiology

View full text Add to dashboard Cite

Enterocytozoon bieneusi causes microsporidiosis, a condition with complex epidemiology involving both direct and indirect transmission routes. To assess the potential role of synanthropic rodents and flies in the transmission of this pathogen, a total of 277 cattle fecal samples, 199 synanthropic rodents, and 50 batches of 20 flies were collected from a cattle farm. These samples were screened for the presence of E. bieneusi by PCR and sequencing of the internal transcribed spacer (ITS) region of the rRNA gene. The positive rates of cattle, synanthropic rodents, and flies were 11.9% (33/277), 4.0% (8/199) and 12.0% (6/50), respectively. Nineteen genotypes were identified, including 11 known genotypes (BEB6, I, COS-I, EbpC, D, J, CHS5, CHG1 to CHG3 and CHG14) and eight novel genotypes (named CHC9 to CHC16). The dominant genotype detected in the present study, BEB6, was found in all three categories of hosts. Moreover, human pathogenic genotypes D and EbpC were also observed in both synanthropic rodents and flies. These results demonstrate that synanthropic rodents and flies may act as biological disseminator or mechanical vector in the transmission of microsporidiosis to humans. Efforts should be made to minimize threats from these commensal animals to public health.

show abstract

High Prevalence and Widespread Distribution of Zoonotic Enterocytozoon bieneusi Genotypes in Swine in Northeast China: Implications for Public Health

Cited by 36 publications

References 41 publications

Multilocus Typing of Enterocytozoon bieneusi in Pig Reveals the High Prevalence, Zoonotic Potential, Host Adaptation and Geographical Segregation in China

Multilocus Typing of Enterocytozoon bieneusi in Pig Reveals the High Prevalence, Zoonotic Potential, Host Adaptation and Geographical Segregation in China

Clonal Evolution of Enterocytozoon bieneusi Populations in Swine and Genetic Differentiation in Subpopulations between Isolates from Swine and Humans

The Potential Role of Synanthropic Rodents and Flies in the Transmission of Enterocytozoon bieneusi on a Dairy Cattle farm in China

Contact Info

Product

Resources

About