High prevalence and widespread distribution of multi-resistant Escherichia coli isolates in pigs and poultry in China

Jiang, Hongxia; Lü, Di; Zhang-liu, Chen; Wang, Xiumei; Chen, Ji-Rong; Liu, Yahong; Liao, Xiao‐Ping; Liu, Jianhua; Zeng, Zhenling

doi:10.1016/j.tvjl.2009.10.017

Cited by 78 publications

(72 citation statements)

References 33 publications

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“…These two molecules (streptomycin and tetracycline) are used very frequently and for a long time in the poultry sector. Studies made by other authors [14,19] were in agreement with our fi ndings. Indeed, they showed a high rates of resistance to tetracycline (90.8-95.2 %), and streptomycin (46 %).…”

Section: Discussionsupporting

confidence: 93%

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Antimicrobial Resistance in Escherichia coli Isolates from Healthy Poultry, Bovine and Ovine in Tunisia: A Real Animal and Human Health Threat

Abbassi¹

2017

J Clin Microbiol Biochem Technol

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A total of 174 E. coli isolates collected from healthy poultry, bovine and ovine recovered between December 2009 and June 2013 in different geographic location in Tunisia, were assessed and examinated for resistance to antimicrobial agents. Avian isolates showed the highest rates of antibiotic resistance: tetracycline (74.7 %), trimethoprim/sulfamethoxazole and amoxicillin with the same rate of resistance (57 %). Prevalences of resistance to the same four antimicrobials in bovine isolates were 33.3 %, 65 %, 30 %, 28.3 %, respectively. However, for ovine isolates, low resistance rates were observed, except for tetracycline (40 %) and amoxicillin (22.85 %). Only one ESBL-producing isolate from chicken was detected. In addition, seventy-seven (44.2 %) isolates were resistant to three or more classes of antibiotics and were considered multidrug resistant (MDR). Interestingly, avian E. coli isolates were more resistant than bovine and ovine ones. These results provide novel insights into the epidemiological characteristics of poultry, bovine and ovine E.coli isolates in Tunisia, and suggest the need for the prudent use of antimicrobial agents in husbandry and the urgent need to establish a national antibiotic resistance monitoring program.

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Section: Discussionsupporting

confidence: 93%

“…were observed worldwide [14,19,37]. For ovine isolates, the frequencies of resistance were low and almost all isolates were susceptible to the tested antibiotics, except for tetracycline (40 %) and to amoxicillin (22,85 %).…”

Section: Discussionmentioning

confidence: 96%

Antimicrobial Resistance in Escherichia coli Isolates from Healthy Poultry, Bovine and Ovine in Tunisia: A Real Animal and Human Health Threat

Abbassi¹

2017

J Clin Microbiol Biochem Technol

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“…These findings were similar with previous studies reporting that TEM β-lactamases were the most frequent mechanism in E. coli isolates from food-producing animals [6,30] .…”

Section: Discussionsupporting

confidence: 93%

Tavuklarda Genişlemiş Spektrumlu β-laktamaz ve AmpC β-laktamaz Üreten Escherichia coli İzolatlarının Saptanması

Kahraman¹,

Sığırcı²,

Çelik³

et al. 2016

Kafkas Univ Vet Fak Derg

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The aim of this study was to investigate the presence of Extended-spectrum β-lactamase (ESBL) and AmpC β-lactamase producing Escherichia coli strains isolated from meat and faecal samples of chicken. Faecal samples (n: 384) were collected from broiler and egg-type healthy chickens in 43 flocks and 384 fresh chicken meat samples from local markets, supermarkets and slaughterhouses. E. coli isolates were tested phenotypically according to the guidelines of CLSI (2013) for ESBL production. Phenotypic detection of AmpC production was carried out by determination of resistance to cefoxitin and susceptibility to cefepime. Also, presence of genes encoding different types of β-lactamases (blaCTX-M, blaTEM, blaSHV and the mutation in the promoter of AmpC) in all phenotypically ESBL or AmpC producing E. coli was investigated by PCR assays. In this study, blaCTX-M (95.4%), blaCTX-M-1 (81.8%), blaCTX-M-8 (4.5%), blaTEM (45.4%) and blaSHV (2.2%) genes were detected from faecal samples and blaCTX-M (100%), blaCTX-M-1 (28.5%) and blaTEM (7.1%) from meat samples. The only plasmidic AmpC β-lactamase found was the CIT type. Keywords: Escherichia coli, ESBL, AmpC, Chicken, PCR Tavuklarda Genişlemiş Spektrumlu β-laktamaz ve AmpC β-laktamaz üreten Escherichia coli İzolatlarının Saptanması ÖzetBu çalışmanın amacı, tavuk eti ve dışkı örneklerinden izole edilen Genişlemiş Spektrumlu β-laktamaz (GSBL) ve AmpC β-laktamaz üreten Escherichia coli izolatlarının varlığını ortaya koymaktır. Bu çalışmada, 43 kümesten sağlıklı tavuklara ait 384 dışkı örneği ve yerel marketlerden, süpermarketlerden ve kesimhanelerden 384 taze tavuk eti örneği toplandı. E. coli izolatları fenotipik olarak CLSI (2013) standartları kullanılarak GSBL üretimi yönünden incelendi. AmpC üretiminin fenotipik olarak saptanması sefoksitine direnç ve sefepime duyarlılık kriterleri ile belirlendi. Fenotipik olarak GSBL ya da AmpC üreten E. coli olarak saptanan izolatlarının hepsi PCR ile değişik tipteki β-laktamaz genlerinin (blaCTX-M, blaTEM, blaSHV, AmpC) varlığını ortaya koymak için incelendi. Bu çalışmada, dışkı örneklerinde blaCTX-M (%95.4), blaCTX-M-1 (%81.8), blaCTX-M-8 (%4.5), blaTEM (%45.4), blaSHV (%2.2) genleri ve tavuk eti örneklerinde ise blaCTX-M (%100), blaCTX-M-1 (%28.5), blaTEM (%7.1) genleri saptandı. AmpC β-laktamaz grubunda sadece CIT geni belirlendi.

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“…Overall, resistance rate to critically important antimicrobials (especially to quinolones) has increased in E. coli sourced from food-producing animals worldwide (Giufre et al, 2012;Halova et al, 2014;Jiang et al, 2011;Lohren et al;Wasyl et al, 2013). The emergence of MDR E. coli isolates, in particular resistance to critically important antimicrobials for humans, is alarming and highlights the need for AMR monitoring systems (Canton et al, 2012;Machado et al, 2008).…”

Section: Antimicrobial Resistance Trend In E Coli From Poultrymentioning

confidence: 99%

“…A positive association has been found between an increase in antimicrobial usage in veterinary medicine and the increasing prevalence of antimicrobial resistant bacteria detected in foodproducing animals and food products (Altalhi et al, 2010;Borjesson et al, 2013;Castellanos et al, 2017;da Costa et al, 2010;Hammerum et al, 2012;Hasan et al, 2011;Hordijk et al, 2013;Jiang et al, 2011;Nilsson et al, 2014;Ojer-Usoz et al, 2013).…”

Section: Public Health Significance Of Apecmentioning

confidence: 99%

Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

Awawdeh¹,

Turni²,

Henning³

et al.

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Avian pathogenic Escherichia coli (APEC) is the causative agent of avian colibacillosis, a localised or systemic infection resulting in clinical diseases such as colisepticemia, chronic respiratory disease and swollen-head syndrome. Globally, avian colibacillosis is the leading cause of morbidity and mortality in poultry, and it has been associated with massive economic losses and welfare problems.This organism is of public health significance as APEC is communicable to humans. The diagnosis of avian colibacillosis relies on clinical signs, typical pathological lesions and culture of E. coli from affected tissue(s). Antimicrobial therapy is often used both for treatment and control. Previous overseas studies have characterised APEC and identified virulence genes (VGs) that can be used as molecular markers for the identification of APEC. Little is known about APEC in broiler chickens in Australia.The aim of this thesis was to gain a better understanding of the epidemiology of APEC in Australian broiler flocks and how factors including presence of VGs and phylogenetic group can improve the identification of this pathotype in Australia. Firstly, three faecal DNA extraction methods were evaluated. Faeces were collected from healthy chickens and chickens with colibacillosis from commercial broiler farms in South East Queensland (SEQ). The extracted DNA was screened by a pentaplex-PCR for five APEC-associated VGs (iroN, iutA, iss, hlyF and ompT). DNA extracted from E. coli isolates cultured from the cloaca and organs of the birds were screened using the same PCR.Repeated bead beating plus column elution was the preferred DNA extraction method, as it yielded good PCR quality and adequate quantity DNA. However, identifying APEC by direct detection of the five VGs from the faecal material was not feasible as all of these genes were also detected in all of the birds. However, the VGs were more commonly detected in E. coli isolates cultured from birds with colibacillosis.A cross-sectional study was performed to estimate APEC farm-level prevalence in healthy broiler chickens in SEQ and to identify potential risk factors associated with the carriage of APEC. At the farm-level, all of the 40 farms sampled were positive for APEC, that is at least one bird per farm carried APEC, while the within-farm prevalence was 63% (95% Confidence Interval: 55.8, 70.2).Higher APEC within-farm bird-level prevalence was significantly associated with the usage of well water as a source of drinking water, failure to disinfect the water line after each flock, farm visitors not showering before entering the shed, distances greater than 20 metres between the car park and the poultry shed and the presence of wild birds within 50 metres of the shed. Chlorinating the drinking water combined with automatic water filtration reduced within-farm bird-level APEC prevalence. Page | 3Therefore, based on the results concluded from the multivariable model, improving biosecurity and water treatments might reduce APEC prevalence, decrease the risk of co...

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High prevalence and widespread distribution of multi-resistant Escherichia coli isolates in pigs and poultry in China

Cited by 78 publications

References 33 publications

Antimicrobial Resistance in Escherichia coli Isolates from Healthy Poultry, Bovine and Ovine in Tunisia: A Real Animal and Human Health Threat

Antimicrobial Resistance in Escherichia coli Isolates from Healthy Poultry, Bovine and Ovine in Tunisia: A Real Animal and Human Health Threat

Tavuklarda Genişlemiş Spektrumlu β-laktamaz ve AmpC β-laktamaz Üreten Escherichia coli İzolatlarının Saptanması

Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

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