High prevalence and molecular characterization of extended-spectrum β-lactamase-producing Proteus mirabilis strains in southern Croatia

Abstract

The aim of this study was to determine the prevalence and antibiotic resistance rates of extendedspectrum b-lactamase (ESBL)-producing Proteus mirabilis strains isolated from inpatients at the Split University Hospital (southern Croatia) during a survey performed between 2005 and 2008. A total of 2152 consecutive isolates of P. mirabilis were isolated. The prevalence was 0.5 % in 2005 and increased significantly to 20.9 % by 2008. Strains were most frequently isolated from urine (36.5 %) and bronchial aspirates and wound swabs (11.3 %). ESBL-producing P. mirabilis isolates showed very high resistance rates to the majority of non-b-lactam antibiotics and were susceptible to a b-lactam/b-lactamase inhibitor and carbapenems. The isolates were genotyped and their ESBLs were molecularly characterized. Strains originating from the intensive care unit and the surgery and neurosurgery wards were clonally related. All P. mirabilis isolates produced the TEM-52 type of ESBL. To the best of our knowledge, our work detailed here and summarized in an earlier communication is the first report of such isolates from southern Croatia. Increased monitoring and screening for ESBL production in this species at our hospital is mandatory.

Multidrug Resistant-Proteus Mirabilis Isolated from Chicken Droppings in Commercial Poultry Farms: Bio-security Concern and Emerging Public Health Threat in Bangladesh

“…The outcomes of this study would be helpful in designing further extensive investigations covering more poultry farms from expanded areas of the country to find nationwide prevalence of this zoonotic human pathogen. Emerging of MDR poultry-origin P. mirabilis has increased over the recent years [8,10,16,26], remains comparable to that of ours. In this study, isolates from chicken droppings showed high resistance properties towards tetracycline, nalidixic acid, ampicillin trimethoprim-sulfamethaxole and gentamycin.…”

“…Such malpractices in antimicrobial uses have been reported to exert selection pressure of antimicrobial resistance to gutmicrobial flora in poultry [13,14]; including recent evidences of multi drug resistance (MDR) P. mirabilis [9]. This P. mirabilis is known human pathogen as a common cause of human urinary tract infection (UTI), nosocomial infection, wound infection [15] and showed clear history of zoonosis in wide host ranges with emergence of MDR in recent years [10,16]. MDR P. mirabilis may therefore be transmitted among PFworkers who in turn may transmit that in surrounding environment thus infecting the catchment population at large.…”

“…Several authors reported presence of P. mirabilis in poultry meat [8,9]; none of similar type of data had been reported from in Bangladesh. Thus the aforementioned facts prompted us to investigate the presence of P. mirabilis in chicken droppings of Bangladeshi poultry that might be transmitting this zoonotic pathogen [10] to vulnerable workers while handing infected chicken directly or through fecal-contaminated poultry products as similarly enterobacteriaceae have been reported to transmit [11,12].…”

The impact of production of extended-spectrum β-lactamases on the 28-day mortality rate of patients with Proteus mirabilis bacteremia in Korea

“…[36, 38–40] Previous studies from 2005 to 2011 in Korea reported incidences of ESBL-producing P. mirabilis of 6.5–12.6%, but no information regarding the change in incidence over time was provided. [16, 18, 43] In this study, the prevalence of ESBL production among P. mirabilis bacteremia isolates was 22.6% over an 8-year period, which is higher than that reported by previous studies in Korea.…”

Isolation of Salmonella species and some other gram negative bacteria from rats cohabitating with poultry in Ibadan, Oyo State, Nigeria

“…Serratia liquefaciens had been implicated in the contamination of chicken carcasses (Lahellec et al, 1975) and it is also occasionally isolated from clinical specimens, although its pathogenic role has not been established (Grimont and Grimont, 1984). The presence of Proteus mirabilis in poultry meat had also been documented (Kim et al, 2005, Wong et al, 2013, and the presence of Proteus species in chicken droppings had been acknowledged as one of the route of its transmission as a zoonotic pathogens to vulnerable workers while handing infected chicken directly (Tonkic et al, 2010) or through fecal-contaminated poultry products as other Enteric bacteria (Lima-Filho et al, 2013). A more current report also associated poultry-borne Proteus species with zoonotic urinary tract infection (Armbruster et al, 2014).It was also associated with rheumatoid arthritis that is common in developing countries (Ebringer and Rashid, 2014).…”