High Prevalence and Diversity of Caliciviruses in a Community Setting Determined by a Metagenomic Approach

Rivera-Gutiérrez, Xaira; Morán, Patricia; Taboada, Blanca; Serrano-Vázquez, Angélica; Iša, Pavel; Rojas-Velázquez, Liliana; Pérez-Juárez, Horacio; López, Susana; Torres, Javier; Ximénez, Cecilia; Arias, Carlos F.

doi:10.1128/spectrum.01853-21

Cited by 5 publications

(5 citation statements)

References 46 publications

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Section: Introductionmentioning

confidence: 72%

“…The oropharynx is a hybrid space shared between the respiratory and digestive tracts, where air but also food and saliva pass through. Thus, it would be expected that at least some microorganisms would be shared between both tracts, as has been shown in previous bacteriome studies in adults 16,17 .Here, we characterized and compared the eukaryotic viral communities present in the oropharynx and feces of nine asymptomatic children during their first year of life, living in a semi-rural community. The diversity and dynamics of the eukaryotic virome in both niches were analyzed in samples collected monthly from each subject to establish a robust longitudinal profile of the changes that occur during this important phase of the infants' development.…”

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confidence: 72%

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The fecal and oropharyngeal eukaryotic viromes of healthy infants during the first year of life are personal

Rivera-Gutiérrez

Morán²,

Taboada³

et al. 2023

Sci Rep

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Using a metagenomic sequencing approach, we described and compared the diversity and dynamics of the oropharyngeal and fecal eukaryotic virome of nine asymptomatic children in a semi-rural community setting located in the State of Morelos, Mexico. Ninety oropharyngeal swabs and 97 fecal samples were collected starting 2 weeks after birth and monthly thereafter until 12 months of age. In both niches, more than 95% of the total sequence reads were represented by viruses that replicate either in humans or in plants. Regarding human viruses, three families were most abundant and frequent in the oropharynx: Herpesviridae, Picornaviridae, and Reoviridae; in fecal samples, four virus families predominated: Caliciviridae, Picornaviridae, Reoviridae, and Anelloviridae. Both niches showed a high abundance of plant viruses of the family Virgaviridae. Differences in the frequency and abundance of sequence reads and diversity of virus species were observed in both niches and throughout the year of study, with some viruses already present in the first months of life. Our results suggest that the children’s virome is dynamic and likely shaped by the environment, feeding, and age. Moreover, composition analysis suggests that the virome composition is mostly individual. Whether this constant exposition to different viruses has a long-term impact on children’s health or development remains to be studied.

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Section: Introductionmentioning

confidence: 72%

mentioning

confidence: 72%

The fecal and oropharyngeal eukaryotic viromes of healthy infants during the first year of life are personal

Rivera-Gutiérrez

Morán²,

Taboada³

et al. 2023

Sci Rep

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“… 35 A high prevalence rate of caliciviruses was detected in asymptomatic children in a study of determining the fecal virome of infants in Mexico. 36 These studies indicate that many enteric infections happen during early childhood without severe clinical symptoms. Diverse enteric viruses were detected in combination with one another in both diarrheal children and healthy children.…”

Section: Discussionmentioning

confidence: 98%

Viral metagenomics of the gut virome of diarrheal children with Rotavirus A infection

Bao

Wang

et al. 2023

Gut Microbes

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Diarrhea is a leading cause of morbidity and mortality in children worldwide and represents a major dysbiosis event. Rotavirus has been recognized as a global leading pathogen of diarrhea. This study is aimed at investigating differences in the gut virome between diarrheal children and healthy controls. In 2018, 76 diarrheal fecal samples and 27 healthy fecal samples in Shanghai and 40 diarrheal fecal samples and 19 healthy fecal samples in Taizhou were collected to investigate the composition of the gut virome. Viral metagenomic analyses revealed that the alpha diversity of the diarrheal virome was not significantly different from that of the healthy virome, and the beta diversity had a significant difference between diarrheal and healthy children. The diarrheal virome was mainly dominated by the families Adenoviridae , Astroviridae , Caliciviridae , and Picornaviridae . Meanwhile, the healthy virome also contains phages, including Microviridae and Caudovirales . The high prevalence of diverse enteric viruses in all samples and the little abundance of Microviridae and Caudovirales in diarrheal groups were identified. The study introduced a general overview of the gut virome in diarrheal children, revealed the compositional differences in the gut viral community compared to healthy controls, and provided a reference for efficient treatments and prevention of virus-infectious diarrhea in children.

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“…Sapovirus causes acute viral gastroenteritis in children <5 years old and can cause outbreaks in enclosed facilities like orphanages. 190 , 191 , 192 Infection of porcine kidney cells (LLCPK) with sapovirus (Cowden strain) caused notably increased COX-2 mRNA and protein levels in a time-dependent manner. 193 Inhibition of COX enzymes by NSAIDs and siRNAs caused markedly reduced PGE 2 levels and significant interference in sapovirus replication.…”

Section: The Potential Influence Of Host-derived Metabolites and Immu...mentioning

confidence: 99%

Candida albicans-enteric viral interactions—The prostaglandin E2 connection and host immune responses

Mochochoko¹,

Pohl²,

O’Neill³

2023

iScience

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High Prevalence and Diversity of Caliciviruses in a Community Setting Determined by a Metagenomic Approach

Abstract: Human caliciviruses are important etiological agents of acute gastroenteritis in children under 5 years of age. Several studies have characterized their association with childhood diarrhea and their presence in nondiarrheal stool samples.

Cited by 5 publications

References 46 publications

The fecal and oropharyngeal eukaryotic viromes of healthy infants during the first year of life are personal

The fecal and oropharyngeal eukaryotic viromes of healthy infants during the first year of life are personal

Viral metagenomics of the gut virome of diarrheal children with Rotavirus A infection

Candida albicans-enteric viral interactions—The prostaglandin E2 connection and host immune responses

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