Summary
The effect of a previous combined treatment (CO2‐enriched modified atmosphere packaging, MAP and high‐pressure processing, HPP, 150 MPa/5 min) on lipid stability of refrigerated (10 days/4 °C) salmon (Oncorhynchus kisutch) was studied. The following processing conditions were compared: B‐0 (fish without MAP or HPP), B‐1 (fish packaged under MAP and without HPP), B‐2 (fish subjected to HPP without MAP) and B‐3 (fish subjected to MAP and HPP). An inhibitory effect (P < 0.05) on lipid hydrolysis and oxidation was obtained by the presence of CO2 in the packaging medium; values detected at day 10 for B‐0 and B‐1 fish were 80.72 and 49.61 (g free fatty acids, FFA, kg−1 lipids), 6.14 and 2.81 (meq active oxygen kg−1 lipids; peroxide value), 5.05 and 3.10 (mg malondialdehyde kg−1 muscle), and 5.56 and 2.70 (fluorescence ratio), respectively. Furthermore, inhibition of lipid damage was observed for HPP alone; values detected at day 10 for B‐2 fish were 76.24 (g FFA kg−1 lipids) and 5.28 (meq active oxygen kg−1 lipids). The lowest average values for lipid hydrolysis and oxidation were obtained in samples corresponding to the combined treatment (B‐3 batch), differences being significant (P < 0.05) at day 10 for FFA (41.43 g kg−1 lipids), peroxide (1.84 meq kg−1 lipids) and fluorescence (2.50) values.