High-pressure liquid chromatographic separation of a mixture of corticosteroids, androgens, and progestins

Huang, Fore‐Lien; Ke, Ferng Chun; Hwang, Juey Jen; Lo, Tung Bin

doi:10.1016/0003-9861(83)90061-9

Cited by 20 publications

(6 citation statements)

References 9 publications

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“…Stress-induced increases in cortisone levels reported here are in line with those previously reported for carp (Huang et al, 1983;Barton & Iwama, 1991). Plasma cortisol levels, however, are higher and the rise in plasma cortisol is faster than reported for rainbow trout at continuous confinement (Pottinger and Moran, 1993) and coho salmon after short handling (Patino, Redding, & Schreck, 1987).…”

Section: Cortisol and Cortisone In Vivosupporting

confidence: 91%

Conservation of Apoptosis as an Immune Regulatory Mechanism: Effects of Cortisol and Cortisone on Carp Lymphocytes

Weyts

Kemenade²,

Flik

et al. 1997

Brain, Behavior, and Immunity

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This is the first study to show that apoptosis as an immune regulatory mechanism is conserved in fish, demonstrating its importance in maintaining immunological homeostasis. The data further show that this mechanism is subject to control by glucocorticosteroids. Carp plasma cortisol concentrations increase from 20 to 434 ng/ml and cortisone from 5 to 50 ng/ ml within 9 min o f the onset o f handling stress. At basal steroid concentrations in vitro, cortisol, but not its conversion product cortisone, inhibits proliferation o f peripheral blood lymphocytes (PBL), as measured by [3H]thymidine incorporation. Induction of apoptosis in activated PBL is the apparent mechanism o f cortisol action. In nonstimulated PBL cultures, apoptosis is induced by neglect (a lack o f stimulating signals). Stimulation with LPS or PHA rescues lymphocytes from this type of apoptosis. Stimulated PBL populations, however, are sensitive to cortisol-induced apoptosis. Culture supematants from activated PBL protect PBL from apoptosis by neglect, probably by supplying a growth signal. These supematants, how ever, have no effect on cortisol-induced apoptosis.

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Section: Cortisol and Cortisone In Vivosupporting

confidence: 91%

Conservation of Apoptosis as an Immune Regulatory Mechanism: Effects of Cortisol and Cortisone on Carp Lymphocytes

Weyts

Kemenade²,

Flik

et al. 1997

Brain, Behavior, and Immunity

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“…The HPLC consisted of a Waters system with a 600 controller, 717 autosampler, 996 photodiode array detector (steroids were monitored at 254 and 280 nm), a Digital Venturis computer, Millenium PDA software and a reverse phase C18 (Hewlett Packard) column. Extracts were examined on HPLC as described by Huang et al (1983) and modified by Feist et al (1990). Briefly, we used an isocratic mobile phase (flow rate 0.4 ml/min) of water : methanol : acetonitrile : isopropanol (62:28:5:5), followed by a linear gradient (3.3%/min) of water : methanol : butanol (35:45:20) for 30 minutes.…”

Section: Hplc Systemmentioning

confidence: 99%

Pacific Lamprey Research and Restoration Project : Annual Report 1999.

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2001

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“…Interacting molecules may be secreted by one cell type in a paracrine mode to exert its effects on neighboring cells or on the extracellular matrix (ECM). 1 Therefore, we attempted to purify the secreted proteins from carp head kidney and eventually to understand the functions of the secreted molecules. Here, we report the purification and cloning of one secreted protein, nephrosin.…”

mentioning

confidence: 99%

Purification and Cloning of Carp Nephrosin, a Secreted Zinc Endopeptidase of the Astacin Family

Hung

Huang

et al. 1997

Journal of Biological Chemistry

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We have purified a secreted proteinase of 23 kDa from carp head kidney by sequential column chromatography on a Reactive Blue 72-agarose dye affinity column and an FPLC Mono-P column. The secretion of this proteinase from carp head kidney can be stimulated by high concentrations of potassium. Since the carp proteinase is present mainly in the head kidney, kidney, and spleen (all of which are lymphohematopoietic organs), it is named nephrosin. The carp nephrosin is most sensitive to metal chelators, but not to inhibitors specific for other classes of proteinases. A cDNA clone has been isolated from a carp head kidney cDNA library by immunoscreening with a polyclonal antiserum raised against purified nephrosin. The cloned cDNA is 1086 base pairs in length and has an open reading frame encoding a protein of 273 amino acids, including a 19-amino acid signal peptide and 56-amino acid propeptide. The deduced amino acid sequence shows moderate levels of identity to medaka HCE1 (52.5%), medaka LCE (50.7%), crayfish astacin (33.2%), murine meprin-␣ (34%), and murine meprin-␤ (33.5%), all members of the astacin family of zinc endopeptidases. Nephrosin is the first member of the astacin family found in lymphohematopoietic tissues.

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High-pressure liquid chromatographic separation of a mixture of corticosteroids, androgens, and progestins

Cited by 20 publications

References 9 publications

Conservation of Apoptosis as an Immune Regulatory Mechanism: Effects of Cortisol and Cortisone on Carp Lymphocytes

Conservation of Apoptosis as an Immune Regulatory Mechanism: Effects of Cortisol and Cortisone on Carp Lymphocytes

Pacific Lamprey Research and Restoration Project : Annual Report 1999.

Purification and Cloning of Carp Nephrosin, a Secreted Zinc Endopeptidase of the Astacin Family

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