High Pressure Liquid Chromatographic Determination of the Intermediates/Side Reaction Products in FD&C Red No. 2 and FD&C Yellow No. 5 : Statistical Analysis of Instrument Response

Abstract

The statistical methods of regression analysis are applied to calibration data obtained by high pressure liquid chromatographic analysis for the intermediates and side reaction products of FD&C Yellow No. 5 and the former FD&C Red No. 2. Equations are presented which allow calculation of the regression line equation, correlation coefficient, the upper prediction limit on the blank (YUB)? t n e limit of detection in terms of concentration (XLD), response above which quantitation is performed (YQ) , and prediction intervals for a specific response. The HPLC methods for FD&C Yellow No. 5 and the former FD&C Red No. 2 are evaluated based on those calculations.

Determination of unsulfonated aromatic amines in D & C Red No. 33 by the diazotization and coupling procedure followed by reversed-phase liquid chromatographic analysis

“…The dilution was required because the response obtained for the higher levels of aniline exceeded the range of the detector. The responses obtained were treated statistically (22) to generate the regression equation and to evaluate the performance of the method. Responses for aniline, 2-ABP, 4-ABP, and 4-AAB were evaluated at 254 and 510 nm.…”

“…The peak heights obtained at 510 and 254 nm and corrected for the color blank were measured at five or six different concentration levels for each amine. The results were treated statistically to calculate the regression equation and to evaluate the limits of determination (22). The levels of amines in commercial materials were determined (254 and 510 nm) from the regression equation.…”