High Pressure Liquid Chromatographic Determination of Carbohydrates in Food Products: Evaluation of Method

Vries, Jonathan W De; Heroff, John C; Egberg, David C.

doi:10.1093/jaoac/62.6.1292

Cited by 10 publications

(9 citation statements)

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“…[ Carbohydrate analysis: For carbohydrate analysis, 2 mL of medium was taken every 2 days, centrifuged for 10 min at 10,000 rpm, and filtered through acrodiscs (0.45-µm pore size, Nalgene®, NY, USA). Sucrose was determined by HPLC according to the previously reported procedure [25].…”

Section: Kinetic Studies and Viability Assaymentioning

confidence: 99%

Production of Honokiol and Magnolol in Suspension Cultures of Magnolia Dealbata Zucc

Domínguez

Chávez

Garduño-Ramı́rez

et al. 2009

Natural Product Communications

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Honokiol and magnolol, important anxiolytic and anti-cancer agents, have been produced in cell-suspension cultures of the endangered Mexican plant Magnolia dealbata Zucc. In vitro cultures of the plant were established, and the accumulation of honokiol and magnolol in callus and cell-suspension cultures was measured. Leaf samples were the best explants for callus establishment and metabolite production, and Murashige and Skoog medium supplemented with 1 mg/L 2, 4-dichlorophenoxyacetic acid and 1 mg/L kinetin yielded 2.3 mg/g of honokiol and 5.9 mg/g of magnolol. Bacterial and fungal contamination was inhibited with a multiple-step tissue sterilization procedure. Oxidation was inhibited with 1 g/L activated charcoal. Cell-suspension batch cultures derived from friable callus obtained from leaves of this species were grown for 30 days in shaker flasks containing Murashige and Skoog medium. Throughout the growth cycle, honokiol and magnolol levels, fresh and dry weight, and sucrose uptake were determined. The effects of carbon source concentration on biomass accumulation and the synthesis of bioactive compounds were studied. By using 3 mL of inocula supplemented with 3% (w/v) sucrose, maximum yields of honokiol (8.1 mg/g) and magnolol (13.4 mg/g) were obtained after 25 days. These yields were 300% and 382%, respectively, of the yields of honokiol and magnolol obtained from field-grown plants.

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Section: Kinetic Studies and Viability Assaymentioning

confidence: 99%

Production of Honokiol and Magnolol in Suspension Cultures of Magnolia Dealbata Zucc

Domínguez

Chávez

Garduño-Ramı́rez

et al. 2009

Natural Product Communications

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“…Comparisons with other results (DeVries et al 1979;Dunmire and Otto 1979;Iverson and Bueno 1981;Picha 1985) are difficult because different foods were tested and precision data were not reported in detail.…”

Section: Recovery Of Added Solutesmentioning

confidence: 99%

“…In addition, extracting dough in water would allow the measurement of sugars and ethanol in a single extract, which is not possible with ethanolic solvents. DeVries et al (1979) extracted a variety of food products in water at 80-90°C, with good recovery of sugars. The Approved Method 80-04 for measuring simple sugars in cereal products involves extraction in boiling water (AACC International 2000).…”

mentioning

confidence: 99%

Simultaneous Extraction and Enzymatic Quantification of Glucose, Sucrose, and Ethanol in Fermenting Yeasted Dough

Loveday

Winger

2008

Cereal Chem

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“…The isocratic acetonitrile-water (78:22, v/v) mobile phase employed with the ELSD to deter mine fructose and glucose did not adequately resolve both monosaccharides due to the dete rioration of the NH 2 column, which is very sus ceptible to water (19). Several changes in the proportions of the mobile phase components were assayed (acetonitrile-water [80:20,82:18, and 85:15, v/v]), and the flow rate was varied between 0.8-1.2 mL/min.…”

Section: Optimization Of Chromatographic Conditions For Ridmentioning

confidence: 99%

High-Performace Liquid Chromatographic Determination of Mono- and Oligosaccharides in Vegetables with Evaporative Light-Scattering Detection and Refractive Index Detection

López-Hernández

González-Castro

Alba

et al. 1998

Journal of Chromatographic Science

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Two high-performance liquid chromatographic methods are compared for the determination of mono-and oligosaccharides in vegetables using an NH2 column and acetonitrile-water as the mobile phase. One method uses a gradient elution and evaporative light-scattering detection; method precision (relative standard deviation) ranges from 1.09 to 4.44%, and detection limits range from 0.014 to 0.061 mg/mL. The second method uses isocratic elution and refractive index detection; method precision (relative standard deviation) ranges from 1.16 to 4.34%, and detection limits range from 0.013 to 0.022 mg/mL. When samples are analyzed by both methods, the difference between the two mean values is not statistically significant.

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High Pressure Liquid Chromatographic Determination of Carbohydrates in Food Products: Evaluation of Method

Cited by 10 publications

References 0 publications

Production of Honokiol and Magnolol in Suspension Cultures of Magnolia Dealbata Zucc

Production of Honokiol and Magnolol in Suspension Cultures of Magnolia Dealbata Zucc

Simultaneous Extraction and Enzymatic Quantification of Glucose, Sucrose, and Ethanol in Fermenting Yeasted Dough

High-Performace Liquid Chromatographic Determination of Mono- and Oligosaccharides in Vegetables with Evaporative Light-Scattering Detection and Refractive Index Detection

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