1976
DOI: 10.1021/ac60370a025
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High-pressure liquid chromatographic determination of ascorbic acid in selected foods and multivitamin products

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Cited by 134 publications
(34 citation statements)
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References 16 publications
(9 reference statements)
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“…The column was a C-18 reverse phase (25 cm× 4.6 mm, 5 μm, Phenomenex, Torrance, CA, USA) and the mobile phase was 30:70 methanol/0.25% KH 2 PO 4 (pH 3.5). The flow rate was 1.0 ml/min and the UV detector was set at 244 nm (Pachla and Kissinger 1976;Sood et al 1976) The antioxidant content of the freeze-dried berries was assessed using the Ferric reducing antioxidant power (FRAP) assay. Freeze-dried berries were measured into amber vials and 15 ml of 100% methanol was added.…”
Section: Fruitmentioning
confidence: 99%
“…The column was a C-18 reverse phase (25 cm× 4.6 mm, 5 μm, Phenomenex, Torrance, CA, USA) and the mobile phase was 30:70 methanol/0.25% KH 2 PO 4 (pH 3.5). The flow rate was 1.0 ml/min and the UV detector was set at 244 nm (Pachla and Kissinger 1976;Sood et al 1976) The antioxidant content of the freeze-dried berries was assessed using the Ferric reducing antioxidant power (FRAP) assay. Freeze-dried berries were measured into amber vials and 15 ml of 100% methanol was added.…”
Section: Fruitmentioning
confidence: 99%
“…Ascorbic acid is widely found in association with various biologically and pharmacologically active substances like acetaminophen [2] and other pharmaceutical products [2][3][4][5]. The determination of AA has been established by such methods as high performance liquid chromatography [6][7][8][9], spectrophotometry [10][11][12], mass spectrometry [13], flow injection [14,15], chemiluminescence [16], and electrochemical methods [17][18][19][20].…”
Section: Introductionmentioning
confidence: 99%
“…For liquid chromatography, reverse-phase ion-exchange columns [11], amino-bonded columns [12], polymer-coated amino columns [13] or, more frequently, octadecyl silane columns [14][15][16], combined with electrochemical, fluorimetric or UV detection, have provided good results for AA analysis of biological, food and pharmaceutical samples, with high sensitivity, specificity and simplicity [8].…”
Section: Introductionmentioning
confidence: 99%