High-Pressure Liquid Chromatographic Determination of Corticosteroids in Topical Pharmaceuticals

Williamsx, Patricia A.; Biehl, Edward R.

doi:10.1002/jps.2600700517

Cited by 27 publications

(1 citation statement)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some works make reference to the simultaneous determination of HA from others compounds in formulations by RP-HPLC [12][13][14][15] and only one mentioned the simultaneous determination of HA and preservatives in the presence of its degradation products [16].…”

Section: Introductionmentioning

confidence: 99%

Validation of a New RP-HPLC Method for Determination of Hydrocortisone Acetate Complexed with HPβCD in a Oral Liquid Pharmaceutical Preparation

RMS¹

2017

JAPLR

View full text Add to dashboard Cite

A simple reversed-phase high-performance liquid chromatographic method (RP-HPLC) for the simultaneous determination of hydrocortisone acetate (HA) complexed with 2-hydroxypropyl-β-cyclodextrin (HPβCD), its degradation products and preservative in an oral liquid pharmaceutical preparation has been validated. The compounds were separated on a 150mm x 4.6mm C 18 column packed with 5μm particles and a 1mm x 4mm pre-column with heating at 30 °C. The mobile phase optimized was a mixture of methanol, acetonitrile and water 35:25:40 (v/v/v), pumped at a flow rate of 1.0mL min -1 . The validation data showed that the proposed method is simple, reliable, fast and has good robustness, specificity, linearity, precision and accuracy.

show abstract

Section: Introductionmentioning

confidence: 99%

Validation of a New RP-HPLC Method for Determination of Hydrocortisone Acetate Complexed with HPβCD in a Oral Liquid Pharmaceutical Preparation

RMS¹

2017

JAPLR

View full text Add to dashboard Cite

show abstract

A rapid and highly sensitive method for the quantitative determination of dexamethasone in plasma, synovial fluid and tissues by combined gas chromatography/negative ion chemical ionization mass spectrometry

Girault

Istin

Fourtillan

1990

Biol. Mass Spectrom.

View full text Add to dashboard Cite

A new, simple and highly sensitive assay is developed for the quantitative measurement of very low levels of dexamethasone in human plasma, synovial fluid and tissues following a topical administration of the drug. Dexamethasone and the internal standard, flumethasone, are measured by gas chromatography/negative ion chemical ionization mass spectrometry with methane as the reagent gas. After a three-step extraction procedure, the two compounds of interest are converted to their trimethylsilyl ether derivatives using trimethylsilylimidazole and formamide as the base catalyst. Under soft derivatization conditions only one chromatographic peak corresponding to the trisubstituted derivative is observed. The mass spectrometer is focused to monitor abundant and stable characteristic high-mass ions (m/z 446 and 464) which are generated in the ion source by an electron capture process. This assay requires only 1 ml of plasma or 0.5 ml of synovial fluid and the detection limit of the method is equal to 0.1 ng ml-1 with a relative standard deviation lower than 6%.

show abstract

Simultaneous Determination of Hydrocortisone and Benzyl Alcohol in Pharmaceutical Formulations by Reversed-Phase High-Pressure Liquid Chromatography

Regox¹,

Nelson²

1982

Journal of Pharmaceutical Sciences

View full text Add to dashboard Cite

High-Pressure Liquid Chromatographic Determination of Corticosteroids in Topical Pharmaceuticals

Cited by 27 publications

References 29 publications

Validation of a New RP-HPLC Method for Determination of Hydrocortisone Acetate Complexed with HPβCD in a Oral Liquid Pharmaceutical Preparation

Validation of a New RP-HPLC Method for Determination of Hydrocortisone Acetate Complexed with HPβCD in a Oral Liquid Pharmaceutical Preparation

A rapid and highly sensitive method for the quantitative determination of dexamethasone in plasma, synovial fluid and tissues by combined gas chromatography/negative ion chemical ionization mass spectrometry

Simultaneous Determination of Hydrocortisone and Benzyl Alcohol in Pharmaceutical Formulations by Reversed-Phase High-Pressure Liquid Chromatography

Contact Info

Product

Resources

About