High Pressure Liquid Chromatographic Determination of 4,4′-(Diazoamino)-dibenzenesulfonic Acid in FD&amp;C Yellow No. 6

Marmion, Daniel M

doi:10.1093/jaoac/60.1.168

Cited by 2 publications

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“…Dyes that have been extensively studied include; tartrazine (Wittmer, Nuessle &Haney, 1975), F D andC Yellow No. 6 (Singh, 1974a;Marmion, 1975Marmion, , 1977Bailey &Cox, 1975) F D andC Red No. 40 (Bailey &Cox, 1976;Singh, 1974b), F D and C Red No.…”

Section: Colourants and Pigmentsmentioning

confidence: 99%

Applications of high pressure liquid chromatography* to food analysis

Macrae¹

1980

Int J of Food Sci Tech

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Summary High pressure liquid chromatography (HPLC) is becoming widely accepted as an invaluable technique for the analysis of many food components. In many instances HPLC methods have replaced laborious analyses and, in general, the chromatographic methods are more specific and precise, coupled with a significant reduction in analysis times. A number of articles, e.g. Battaglia (1977), Rapp & Ziegler (1975), Wildanger (1975a) and Saxby (1978a,b), have been written reviewing the applications of HPLC to food analysis, but these have not covered the literature comprehensively. This is attempted here, preceded by a brief description of the methodology and instrumentation of HPLC.

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Section: Colourants and Pigmentsmentioning

confidence: 99%

Applications of high pressure liquid chromatography* to food analysis

Macrae¹

1980

Int J of Food Sci Tech

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Determination of Seven Organic Impurities in FD&C Yellow No. 6 by Ultra-High-Performance Liquid Chromatography

Stover

Harp

2021

Journal of AOAC INTERNATIONAL

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Background The U.S. Food and Drug Administration (FDA) batch certifies FD&C Yellow No. 6 (Y6) to ensure that the color additive meets requirements published in the Code of Federal Regulations, including specifications for seven organic manufacturing impurities consisting of two intermediates, a reaction by-product, an impurity originating from an intermediate, and three subsidiary colors. Objective An ultra-high-performance liquid chromatography (UHPLC) method was developed and validated for determining seven organic impurities in Y6. For comparison, the currently used high-performance liquid chromatography (HPLC) method was also validated. Methods The new UHPLC method uses a 1.7 µm particle size biphenyl column with aqueous ammonium formate and methanol as eluants. Analytes are identified by comparing their retention times and UV-visible spectra to those of reference standards. Calibration is performed in the presence of the dye matrix and analyte levels are determined from their peak areas. Results UHPLC and HPLC validation studies obtained linear calibration curves and excellent values for limits of detection, limits of quantitation, recovery, repeatability, and intermediate precision for all analytes. Survey analyses of 30 samples using the UHPLC and HPLC methods yielded results that were consistent within experimental error; however, the UHPLC method provided more accurate results for two analytes that coelute using the HPLC method. Conclusions The UHPLC method satisfies the accuracy and precision requisites for routine certification of Y6. Highlights The UHPLC method is faster and generates less waste than the HPLC method while delivering better sensitivity, separation, and accuracy.

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High Pressure Liquid Chromatographic Determination of 4,4′-(Diazoamino)-dibenzenesulfonic Acid in FD&C Yellow No. 6

Cited by 2 publications

References 0 publications

Applications of high pressure liquid chromatography* to food analysis

Applications of high pressure liquid chromatography* to food analysis

Determination of Seven Organic Impurities in FD&C Yellow No. 6 by Ultra-High-Performance Liquid Chromatography

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