High-Pressure Liquid Chromatographic Assay of Chloramphenicol in Biological Fluids

Sample, R H; Glick, Melvin R.; Kleiman, Martin B.; Smith, James W.; Oei, T O

doi:10.1128/aac.15.3.491

Cited by 19 publications

(4 citation statements)

References 10 publications

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“…Drug peaks with shoulders or widened bases should be studied for co-eluting drugs. By using new high-efficiency col umns of 5-pm particle size, previous problems [Sample et al, 1979] of coeluting peaks are seldom encountered.…”

Section: Discussionmentioning

confidence: 99%

“…Because there are few drug assay kits available, many drugs must be quantified using any number of analytical instruments. Although there are hundreds of methods available for quantifying drugs, many using the HPLC [Gal et al, 1980;Sample et al, 1979], we present an alternative approach by using the same mobile phase and analytical method to analyze a large number of drugs commonly used in pediatric medicine. By merely changing the mobile phase compo sition to provide optimal elution from the column, most common drugs can be quantified.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Therapeutic Drug Monitoring in a Pediatric Laboratory

Hale¹,

Shum²,

Habersang³

1987

Dev Pharmacol Ther

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Therapeutic drug monitoring using a new and faster high pressure liquid chromatographic (HPLC) method is discussed. Analysis of chloramphenicol and succinate esters and a large number of anticonvulsive drugs can be carried out using the same buffer and reverse-phase HPLC column by changing the solvent concentration to reflect the choice of drugs. A buffer prepared from 0.02 M sodium acetate and trifluoroacetic acid provides an optimal mobile phase for separation of a wide variety of drugs commonly used in pediatric medicine. Sample preparation and the use of special internal standards without extensive extractions provides excellent accuracy, reliability and sensitivity, and requires a minimum of preparation.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Therapeutic Drug Monitoring in a Pediatric Laboratory

Hale¹,

Shum²,

Habersang³

1987

Dev Pharmacol Ther

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“…HPLC methods for determination and quantification of CAM (Boer and Pijnenburg, 1983;Burke et al, 1980;Ingebrigtsen et al, 2016;Sample et al, 1979) and BPO (Burton et al, 1979;Saiz et al, 2001) as a single drug have already been described in the literature. However, no reports on codetermination were available and we aimed to develop a time-efficient HPLC method capable of simultaneous detection of the two drugs.…”

Section: Simultaneous Detection Of Bpo and Cammentioning

confidence: 99%

Successful co-encapsulation of benzoyl peroxide and chloramphenicol in liposomes by a novel manufacturing method - dual asymmetric centrifugation

Ingebrigtsen

Škalko‐Basnet

Jacobsen

et al. 2017

European Journal of Pharmaceutical Sciences

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Encapsulation of more than one active pharmaceutical ingredient into nanocarriers such as liposomes is an attractive approach to achieve a synergic drug effect and less complicated dosing schedules in multi-drug treatment regimes. Liposomal drug delivery in acne treatment may improve drug efficiency by targeted delivery to pilosebaceous units, reduce adverse effects and improve patient compliance. We therefore aimed to co-encapsulate benzoyl peroxide (BPO) and chloramphenicol (CAM) into liposomes using the novel liposome processing method - dual asymmetric centrifugation (DAC). Liposomes were formed from soybean lecithin, propylene glycol and distilled water (2:1:2w/v/v ratio), forming a viscous liposome dispersion. Liposomes containing both drugs (BPO-CAM-Lip), single drug (BPO-Lip and CAM-Lip), and empty liposomes were prepared. Drug entrapment of BPO and CAM was determined by a newly developed HPLC method for simultaneous detection and quantification of both drugs. Encapsulation of around 50% for BPO and 60% for CAM respectively was obtained in both single-drug encapsulated formulations (BPO-Lip and CAM-Lip) and co-encapsulated formulations (BPO-CAM-Lip). Liposome sizes were comparable for all liposome formulations, ranging from 130 to 150nm mean diameter, with a polydispersity index <0.2 for all formulations. CAM exhibited a sustained release from all liposomal formulations, whereas BPO appeared retained within the liposomes. BPO retention could be attributed to its poor solubility. However, HaCaT cell toxicity was found dependent on BPO released from the liposomes. In the higher concentration range (4%v/v), liposomal formulations were less cytotoxic than the corresponding drug solutions used as reference. We have demonstrated that DAC is a fast, easy, suitable method for encapsulation of more than one drug within the same liposomes.

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“…So far, several techniques for detecting CAP drug residue have been developed, for example, photoinduced chemiluminescence, high-performance liquid chromatography (HPLC) [ 7 ], liquid chromatography (LC) [ 8 ], liquid chromatography–mass spectrometry (LC-MS) [ 9 ], and gas chromatography–mass spectrometry (GC-MS) [ 10 ] etc. Although these methods are reliable, they are accompanied by the disadvantages of cumbersome instrumentation, complicated operation, and high cost.…”

Section: Introductionmentioning

confidence: 99%

MXene–AuNP-Based Electrochemical Aptasensor for Ultra-Sensitive Detection of Chloramphenicol in Honey

Yang

Zhong

et al. 2022

Molecules

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A simple and label-free electrochemical aptasensor was developed for ultra-sensitive determination of chloramphenicol (CAP) based on a 2D transition of metal carbides (MXene) loaded with gold nanoparticles (AuNPs). The embedded AuNPs not only inhibit the aggregation of MXene sheets, but also improve the quantity of active sites and electronic conductivity. The aptamers (Apts) were able to immobilize on the MXene–AuNP modified electrode surface through Au–S interaction. Upon specifically binding with CAP with high affinity, the CAP–Apt complexes produced low conductivity on the aptasensor surface, leading to a decreased electrochemical signal. The resulting current change was quantitatively correlated with CAP concentration. Under optimized experimental conditions, the constructed aptasensor exhibited a good linear relationship within a wide range of 0.0001–10 nM and with a low detection limit of 0.03 pM for CAP. Moreover, the developed aptasensor has been applied to the determination of CAP concentration in honey samples with satisfactory results.

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High-Pressure Liquid Chromatographic Assay of Chloramphenicol in Biological Fluids

Abstract: Chloramphenicol can be assayed by a sensitive and selective high-pressure liquid chromatographic assay technique. The method described is easily adapted to analysis of many other drugs.

Cited by 19 publications

References 10 publications

Therapeutic Drug Monitoring in a Pediatric Laboratory

Therapeutic Drug Monitoring in a Pediatric Laboratory

Successful co-encapsulation of benzoyl peroxide and chloramphenicol in liposomes by a novel manufacturing method - dual asymmetric centrifugation

MXene–AuNP-Based Electrochemical Aptasensor for Ultra-Sensitive Detection of Chloramphenicol in Honey

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