High pressure-induced changes in bovine milk proteins: A review

Huppertz, Thom; Fox, Patrick F.; Kruif, Kees G. de; Kelly, Alan L.

doi:10.1016/j.bbapap.2005.11.010

Cited by 152 publications

(113 citation statements)

References 41 publications

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“…The principal heatinduced change in milk proteins, denaturation of whey proteins, as discussed earlier, also occurs under pressure, with β-lactoglobulin being more susceptible to denaturation, at pressures above 100 MPa (the typical units of pressure, where 1 MPa is 10 times atmospheric pressure), than α-lactalbumin, which is significantly denatured only at or above 400 MPa [65].…”

Section: High-pressure Treatment Of Cheese-milkmentioning

confidence: 99%

“…Depending on the pressure applied, duration and temperature of treatment and pH of milk, HP treatment of milk can result in aggregation of casein micelles (around 250 MPa) or significant dissociation (at pressures above 400 MPa). These phenomena have been shown to be due to the extent to which reassociation occurs after varying extents of pressure-induced disruption of the cohesive forces maintaining the structural integrity of casein micelles, e.g., the extent of solubilisation of colloidal calcium phosphate (CCP) [65].…”

Section: High-pressure Treatment Of Cheese-milkmentioning

confidence: 99%

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Pre-treatment of cheese milk: principles and developments

Kelly

Huppertz

Sheehan

2008

Dairy Sci. Technol.

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-Classically, very few pre-treatments are applied to milk for cheese-making, with some cheese varieties simply made from raw whole milk, but most made from pasteurised milk of which the composition (e.g., fat:protein ratio) may have been standardized. However, there has been consistent interest in more novel and sophisticated strategies for pre-treatment of cheese-milk. Approaches explored include the use of alternative processing technologies (e.g., membrane filtration, high-pressure treatment, homogenisation, heat treatments more severe than pasteurisation) or addition of sources of protein or milk solids (e.g., milk powders, whey protein products) or enzymes. The principal reasons for such pre-treatments of cheese-milk are: (1) to control the microbiology of the raw milk and the resulting cheese better than is possible by pasteurisation (e.g., inactivation or removal of spores, control of non-starter lactic acid bacteria); (2) increasing the yield of cheese, e.g., through heat-or pressure-induced incorporation of whey proteins, or enhancing sensory properties of reduced fat cheese by direct addition of microparticulated whey proteins; (3) manipulation of cheese ripening, e.g., reducing the likelihood of off-flavour development by inactivation of enzymes or accelerating ripening through increasing enzyme-substrate interactions; or (4) improving the texture and other functional properties, e.g., melting. Finally, the considerations for manufacture and ripening of different cheese varieties, or sub-classes of specific varieties (e.g., low-fat cheese) will clearly differ and add to the complexity of the technological options available. This article will review the key principles for pre-treatment of cheese-milk, as summarised briefly above. Résumé -Pré-traitement du lait de fabrication de fromage : principes et avancées.Classiquement, très peu de pré-traitements sont appliqués au lait de fabrication de fromage : quelques variétés de fromage sont faites simplement à partir de lait entier cru, mais la plupart d'entre elles sont fabriquées à partir de lait pasteurisé dont la composition (par exemple rapport matière grasse/protéine) a pu être standardisée. Cependant, un intérêt constant s'est manifesté pour des stratégies nouvelles et plus sophistiquées pour le pré-traitement du lait de fromagerie. Les approches explorées incluent l'utilisation de technologies de traitement alternatives (par exemple filtration membranaire, haute pression, homogénéisation, traitements thermiques plus sévères que la pasteurisation) ou addition de sources de protéines ou de matière sèche de lait (par exemple poudres de lait, protéines de lactosérum) ou d'enzymes. Les principales raisons de tels pré-traitements du lait de fabrication fromagère sont : (1) de contrôler la microbiologie du lait cru et du fromage résul-tant, mieux que ce qui est possible par pasteurisation (par exemple inactivation ou retrait des spores, contrôle des bactéries lactiques non levain) ; (2) d'accroître le rendement en fromage, par exemple en induisant...

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Section: High-pressure Treatment Of Cheese-milkmentioning

confidence: 99%

Section: High-pressure Treatment Of Cheese-milkmentioning

confidence: 99%

Pre-treatment of cheese milk: principles and developments

Kelly

Huppertz

Sheehan

2008

Dairy Sci. Technol.

Self Cite

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“…Some studies on the influence of pressure on pure β-lactoglobulin have indicated that high pressure had a notable effect on its conformational and aggregation properties, affecting its functionality [13]. However, functionality of WPC and WPI modified by high pressure still does not meet the expectations which could realise the full potential of these food ingredients in industrial application [23,31].…”

Section: Introductionmentioning

confidence: 99%

Effects of high pressure on functionality of whey protein concentrate and whey protein isolate

Krešić

Lelas

Herceg

et al. 2006

Lait

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-The objective of this study was to evaluate the influence of high-pressure treatments on the solubility, surface hydrophobicity, foaming and emulsifying ability of whey protein concentrate (WPC) and whey protein isolate (WPI). Dispersions of WPC and WPI powders (10% (w/w)) were processed at 300 MPa and 600 MPa, for 5 and 10 min at 40 ± 2°C. Changes in protein solubility were determined as solubility at pH 7.0 and at pH 4.6. Assessment of foaming properties was based on the foam expansion during prolonged whipping, and foam stability. Emulsifying properties were characterised by emulsion stability and emulsifying activity indices. The results show significant (P < 0.05) modification of solubility and surface hydrophobicity with increasing intensity and duration of applied pressure, indicating partial denaturation and aggregation of proteins. It was found that high-pressure treatments significantly (P < 0.05) improved the foaming behaviour of WPI, while the foaming ability of WPC was diminished. However, foams formed with high-pressuretreated WPC and WPI exhibited significantly prolonged stability (P < 0.05) compared with control samples. There was a significant trend of decreasing emulsifying efficiency and emulsifying stability related to the intensity of applied pressure and treatment time for both WPC and WPI. emulsifying properties / foaming properties / high pressure / whey protein concentrate / whey protein isolate / solubility / surface hydrophobicity 摘要 -高压处理对乳清浓缩蛋白和乳清分离蛋白的影响。本文研究了高压处理对乳清浓缩蛋白 (WPC) 和乳清分离蛋白 (WPI) 的溶解性、表面疏水性、发泡性和乳化性的影响。在 40°C 下，乳清浓缩蛋白 (10 %, w/w) 和乳清分离蛋白 (10 %, w/w) 的分散液分别在 300 MPa 和 600 Mpa 下高压处理 5 min 和 10 min。在 pH 7.0 和 pH 4.6 测定了两种蛋白质溶解性的变化。以长时间搅打后形成的泡沫膨胀度和泡沫稳定性来评价蛋白质的发泡性能；根据测定乳液稳定性指数和乳化活性指数评价两种蛋白质的乳化特性。试验结果表明，经过高压长时间的处理后，由于蛋白质发生了部分变性和凝聚作用，使得蛋白质的溶解性和表面疏水性发生了显著的改变 (P < 0.05)。高压处理能够显著地改善 WPI 的发泡性 (P < 0.05)，而 WPC 的泡沫形成能力则降低。然而与对照样品相比，经过高压处理后的 WPC 和 WPI 形成的泡沫稳定性有显著提高 (P < 0.05)。随着处理压力和作用时间的增加，两种蛋白质的乳化性和乳化稳定性均呈下降的趋势。乳化性 / 发泡性 / 高压 / 乳清浓缩蛋白 / 乳清分离蛋白 / 溶解性 / 表面疏水性 * Corresponding author (通讯作者): Greta.Kresic@fthm.hrArticle published by EDP Sciences and available at http://www.edpsciences.org/lait or http://dx

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“…Protein is probably the most valuable constituent of milk, due to its high nutritional quality and unique physico-chemical and functional properties. These properties are fundamental to the production and characteristics of many dairy products, such as cheese or yogurt [19,35].…”

Section: Introductionmentioning

confidence: 99%

Impact of ultrasound on dairy spoilage microbes and milk components

Cameron

McMaster

Britz

2009

Dairy Sci. Technol.

131

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-Numerous reports in the literature suggest pasteurisation failures in the dairy industry as a possible cause for an end product with a poor quality. Ultrasonication offers the dairy industry a non-thermal alternative to pasteurisation. The aim of this study was to evaluate the use of ultrasonication as an alternative to heat pasteurisation. Ultrasound was found to eliminate spoilage and potential pathogens to zero or to levels acceptable by South African and British milk legislation, even when initial inoculum loads of 5× higher than permitted were present before treatment. Viable cell counts of E. coli were reduced by 100% after 10.0 min of ultrasonication. The data obtained also showed that viable counts of Pseudomonas fluorescens were reduced by 100% after 6.0 min and Listeria monocytogenes was reduced by 99% after 10.0 min. An infra-red based apparatus was used to analyse raw and pasteurised milk after an ultrasonic treatment. Ultrasonication did not lead to decreases in the protein or lactose content of both raw and pasteurised milk. Kjeldahl nitrogen determinations confirmed that ultrasonication had no detrimental effect on the total protein or casein content of pasteurised milk. This study indicated that ultrasonication lead to an increase in the fat concentration. This was explained by the larger surface area of the fat globules after ultrasonication, which led to an increase in light scattering as observed by the MilkoScan. Alkaline phosphatase and lactoperoxidase activities were also investigated as potential indicators of an effective ultrasonic treatment. Ultrasonication was, however, found to be ineffective in deactivating both enzymes used regularly by the dairy industry as indicators of effective thermal processes. Résumé -Impact des ultrasons sur les microbes d'altération du lait et sur ses composants.De nombreuses publications suggèrent que dans l'industrie laitière, la mauvaise qualité du produit fini peut provenir de défaillances de la pasteurisation. Le but de cette étude a été d'évaluer l'utilisation de l'ultrasonication comme alternative au traitement thermique. Les résultats ont montré que les ultrasons éliminaient totalement, ou à des niveaux acceptables par la législation sud-africaine et anglaise, la flore d'altération et les pathogènes potentiels, et ce même quand les charges d'inoculum initiales avant traitement étaient 5 fois supérieures à celles autorisées. La réduction du nombre de cellules viables après ultrasonication était de 100 % après 10.0 min pour E. coli, de 100 % après 6.0 min pour Pseudomonas fluorescens et de 99 % après 10.0 min pour Listeria monocytogenes. L'analyse par infrarouge du lait cru et du lait pasteurisé après traitement aux ultrasons ne montrait aucune diminution de la teneur en protéines et en lactose dans ces deux laits. La quantification de l'azote par Kjeldahl confirmait l'absence d'effet préjudiciable de l'ultrasonication sur les teneurs en protéines totales ou en caséines du lait pasteurisé. Cette étude montre que l'ultrasonication conduit à ...

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High pressure-induced changes in bovine milk proteins: A review

Cited by 152 publications

References 41 publications

Pre-treatment of cheese milk: principles and developments

Pre-treatment of cheese milk: principles and developments

Effects of high pressure on functionality of whey protein concentrate and whey protein isolate

Impact of ultrasound on dairy spoilage microbes and milk components

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