High Pressure Homogenizer in Pharmaceuticals: Understanding Its Critical Processing Parameters and Applications

Yadav, Khushwant S.; Kale, Ketaki

doi:10.1007/s12247-019-09413-4

Cited by 55 publications

(32 citation statements)

References 50 publications

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“…Starting with these observations, we can hypothesize the overlapping of several phenomena in the phases. As previously reported on liposomes and oil-in-water emulsions treated by HPH [40][41][42][43][44][45], we may suppose that in the first phase, shear forces cut the bilayer membrane only of larger vesicles, reducing the polydispersity and, therefore, the principal peak value of PSD. Moreover, we have to consider that a small number of amphiphiles in aqueous solution could re-assembly to reduce their thermodynamic energy, forming smaller bilayer structures without compromising the role of naive Exos.…”

Section: Dynamic High-pressure Homogenization Effect On Exos Stabilitysupporting

confidence: 61%

A High Throughput Approach Based on Dynamic High Pressure for the Encapsulation of Active Compounds in Exosomes for Precision Medicine

Romano

Netti

Torino

2021

IJMS

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In recent decades, endogenous nanocarrier-exosomes have received considerable scientific interest as drug delivery systems. The unique proteo-lipid architecture allows the crossing of various natural barriers and protects exosomes cargo from degradation in the bloodstream. However, the presence of this bilayer membrane as well as their endogenous content make loading of exogenous molecules challenging. In the present work, we will investigate how to promote the manipulation of vesicles curvature by a high-pressure microfluidic system as a ground-breaking method for exosomes encapsulation. Exosomes isolated from Uppsala 87 Malignant Glioma (U87-MG) cell culture media were characterized before and after the treatment with high-pressure homogenization. Once their structural and biological stability were validated, we applied this novel method for the encapsulation in the lipidic exosomal bilayer of the chemotherapeutic Irinotecan HCl Trihydrate-CPT 11. Finally, we performed in vitro preliminary test to validate the nanobiointeraction of exosomes, uptake mechanisms, and cytotoxic effect in cell culture model.

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Section: Dynamic High-pressure Homogenization Effect On Exos Stabilitysupporting

confidence: 61%

A High Throughput Approach Based on Dynamic High Pressure for the Encapsulation of Active Compounds in Exosomes for Precision Medicine

Romano

Netti

Torino

2021

IJMS

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“…The special and correct connection of molecular fluids transferred through the HPH device causes the development of colloidal formulation with eligible pharmaceutical applications [30].…”

Section: Introductionmentioning

confidence: 99%

“…In HPH the liquid premix is passed through a narrow split under high pressure. This premix could be a coarse emulsion, micro-suspension, or dispersion [30]. The essential factor for size reduction and droplet breakup is homogenizing pressure.…”

Section: Introductionmentioning

confidence: 99%

“…The essential factor for size reduction and droplet breakup is homogenizing pressure. when the premix passes under high pressure through a small orifice [30]. During the transmission of liquids through the HPH, the collected molecules that are associated with both the liquid and solid interacting could change the molecular dynamics of liquids [30,31].…”

Section: Introductionmentioning

confidence: 99%

“…when the premix passes under high pressure through a small orifice [30]. During the transmission of liquids through the HPH, the collected molecules that are associated with both the liquid and solid interacting could change the molecular dynamics of liquids [30,31]. Understanding the importance of HPH processing parameters is essential due to the impact of these changes on physicochemical properties such as size, dispersion index (PDI) and surface charge, which affects the stability and cellular uptake [32].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Preparation of cationized albumin nanoparticles loaded indirubin by high pressure hemogenizer

Nekounam

Dinarvand

Khademi

et al. 2021

Preprint

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Indirubin can be applied as an anti-cancer drug for inhibition of brain tumors. However, its performance is reduced due to hydrophobicity. In this study, we synthesized cationic human serum albumin (CHSA) nanoparticle by a new hybrid approach for improvement the surface chemistry of albumin and investigate the amount of indirubin loaded CHSA nanoparticle. In this study, the generated mechanical force from a high-pressure homogenizer (HPH) was used to make nanoparticles with a certain size with narrow polydispersity. The results indicated that the size of indirubin loaded CHSA nanoparticles were 130 nm and their zeta potential were +9. Besides, the encapsulation efficiency and drug loading capacity were found to be 85% and 5.8 %, respectively. To the best to our knowledge, this is the first time that indirubin has been used in albumin nanoparticles. In this study, indirubin loaded CHSA nanoparticles was shown can be a potential candidate for drug delivery in the treatment of glioblastoma. Moreover, the cationized form allows the chemical agent to be transmitted to the brain.

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Protein Nanoparticles: Uniting the Power of Proteins with Engineering Design Approaches

Habibi

Mauser

et al. 2022

Advanced Science

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Protein nanoparticles, PNPs, have played a long‐standing role in food and industrial applications. More recently, their potential in nanomedicine has been more widely pursued. This review summarizes recent trends related to the preparation, application, and chemical construction of nanoparticles that use proteins as major building blocks. A particular focus has been given to emerging trends related to applications in nanomedicine, an area of research where PNPs are poised for major breakthroughs as drug delivery carriers, particle‐based therapeutics or for non‐viral gene therapy.

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High Pressure Homogenizer in Pharmaceuticals: Understanding Its Critical Processing Parameters and Applications

Cited by 55 publications

References 50 publications

A High Throughput Approach Based on Dynamic High Pressure for the Encapsulation of Active Compounds in Exosomes for Precision Medicine

A High Throughput Approach Based on Dynamic High Pressure for the Encapsulation of Active Compounds in Exosomes for Precision Medicine

Preparation of cationized albumin nanoparticles loaded indirubin by high pressure hemogenizer

Protein Nanoparticles: Uniting the Power of Proteins with Engineering Design Approaches

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